Detection of Toxic Shock Syndrome Toxin-1 Gene in Staphylococcus aureus Bovine Isolates and Bulk Milk by the Polymerase Chain Reaction.

Takeuchi, Shinichi; Ishiguro, Keiko; Ikegami, Mitsuhiro; Kaidoh, Toshiö; Hayakawa, Yûji

doi:10.1292/jvms.58.11_1133

Cited by 8 publications

(4 citation statements)

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“…The involvement of enterotoxin G-and I-producing S. aureus strains had been previously demonstrated for S. aureus isolates from humans with staphylococcal toxic shock syndrome and staphylococcal scarlet fever (19). Production of SEA, SEB, SED, SEE, and TSST-1 by S. aureus strains associated with bovine mastitis has been described by numerous authors (8,24,27,39,40,44). It was interesting that all SEC producers of the present study were TSST-1 positive and all SED producers were SEJ positive.…”

Section: Discussionsupporting

confidence: 66%

Toxin Genes and Other Characteristics of Staphylococcus aureus Isolates from Milk of Cows with Mastitis

Akineden

Annemüller

Hassan

et al. 2001

Clin Diagn Lab Immunol

194

143

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In the present study, 103 Staphylococcus aureus strains isolated from milk samples from 60 cows with mastitis from eight different farms in seven different locations in one region of Germany were compared pheno-and genotypically and by identification of various toxins. On the basis of culture and hemolytic properties and by determination of the tube coagulase reaction, all of the isolates could be identified as S. aureus. This could be confirmed by PCR amplification of species-specific parts of the gene encoding the 23S rRNA. In addition, all of the S. aureus isolates harbored the genes encoding staphylococcal coagulase and clumping factor and the genes encoding the X region and the immunoglobulin G binding region of protein A. These four genes displayed size polymorphisms. By PCR amplification, the genes for the toxins staphylococcal enterotoxin A (SEA), SEC, SED, SEG, SEI, SEJ, and TSST-1 but not those for SEB, SEE, SEH, and the exfoliative toxins ETA and ETB could be detected. To analyze the epidemiological relationships, the isolates were subjected to DNA fingerprinting by macrorestriction analysis of their chromosomal DNAs. According to the observed gene polymorphisms, the toxin patterns, and the information given by macrorestriction analysis of the isolates by pulsedfield gel electrophoresis, a limited number of clones seemed to be responsible for the cases of bovine mastitis on the various farms.

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Section: Discussionsupporting

confidence: 66%

Toxin Genes and Other Characteristics of Staphylococcus aureus Isolates from Milk of Cows with Mastitis

Akineden

Annemüller

Hassan

et al. 2001

Clin Diagn Lab Immunol

194

143

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“…The results are in agreement with other studies which also did not report high frequency of this gene. Takeuchi et al (1996) performed a study on 125 bovine bulk milk samples and they detected the TSST-1 gene in 10 (8%) of the samples using PCR. Tsen et al (1998) employed a PCR assay and identified that 3 (4.8%) out of 62 strains of S. aureus from clinical sources were tst-carrying strains in Taiwan.…”

Section: Discussionmentioning

confidence: 99%

Detection of toxic shock toxin (tst) gene in Staphylococcus aureus isolated from bovine milk samples

Baniardalan¹,

Mohammadzadeh²,

Pajohi‐Alamoti³

et al. 2017

BJVM

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Staphylococcus aureus is a major causative pathogen of clinical and subclinical mastitis in dairy cattle all over the world. This agent produces a variety of extracellular toxins and virulence factors including toxic shock syndrome toxin-1 (TSST-1) which is the major cause of toxic shock syndrome (TSS). In the present study, 76 S. aureus isolates have been obtained from milk samples collected from 7 dairy herds in Hamedan province of Iran. The isolates were identified based on the biochemical and molecular methods using PCR amplification of the femA gene. The staphylococcal isolates were also examined for the presence of TSST-1 (tst) encoding gene. This gene was detected in only one S. aureus isolate (1.3%). The results revealed that S. aureus strains causing bovine mastitis may potentially produce staphylococcal toxic shock syndrome toxin-1, indicating that it is very important to follow the presence of TSST-1 producing S. aureus isolates in foodstuffs to protect consumers against the risk of toxic shock syndrome.

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“…A method based on real‐time PCR using the LightCycler FastStart DNA Master SYBR Green I kit (Roche Diagnostics, Indianapolis, USA) was employed using the SmartCycler II instrument (Cepheid, Sunnyvale, CA, USA) to detect pvl , tst , and hla . Primers for pvl were adopted from McDonald et al, for tst from Takeuchi et al and for hla from Lee et al . The presence of sea , seb , sec , sed , see , seg , seh , sei, and sej was determined by the multiplex PCR method adopted from Sauer et al .…”

Section: Methodsmentioning

confidence: 99%

Molecular characterization of Staphylococcus aureus isolates from skin and soft tissue infections samples and healthy carriers in the Central Slovenia region

Švent-Kučina

Pirš

Kofol

et al. 2016

APMIS

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Staphylococcus aureus is among the most important human pathogens. It is associated with different infections and is a major cause of skin and soft tissue infections (SSTIs). The aim of our study was to compare S. aureus isolates associated with SSTIs with isolates obtained from healthy carriers in the Central Slovenia region in terms of antimicrobial susceptibility, genetic diversity by clonal complex (CC)/sequence type, spa type, and by toxin gene profiling. In total, 274 S. aureus isolates were collected prospectively by culturing wound samples from 461 SSTI patients and nasal samples from 451 healthy carriers. We have demonstrated high heterogeneity in terms of CCs and spa type in both groups of isolates. The main clone among SSTI strains was Panton-Valentine leukocidin gene (pvl) positive CC121, whereas the main clone among carrier strains was CC45 carrying a large range of toxin genes. The main spa type in both groups was t091. Pvl was more frequently present in SSTI strains (31.2% SSTI vs 3.6% carrier strains) and staphylococcal enterotoxin C was more frequently present in carrier strains (1.6% SSTI vs 17.0% carrier strains). We have also demonstrated that methicillin-resistant S. aureus was a rare cause (2.8%) of SSTIs in our region.

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Detection of Toxic Shock Syndrome Toxin-1 Gene in Staphylococcus aureus Bovine Isolates and Bulk Milk by the Polymerase Chain Reaction.

Cited by 8 publications

References 0 publications

Toxin Genes and Other Characteristics of Staphylococcus aureus Isolates from Milk of Cows with Mastitis

Toxin Genes and Other Characteristics of Staphylococcus aureus Isolates from Milk of Cows with Mastitis

Detection of toxic shock toxin (tst) gene in Staphylococcus aureus isolated from bovine milk samples

Molecular characterization of Staphylococcus aureus isolates from skin and soft tissue infections samples and healthy carriers in the Central Slovenia region

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