Detection of Toxigenic and Atoxigenic Strains of Aspergillus flavus in Telangana and Andhra Pradesh

Teja, M. Ravi; Kumar, K. V. K.; Srilakshmi, P.; Sudini, Harikishan; Varma, P Kishore; Rao, Rakesh

doi:10.18782/2320-7051.3091

Cited by 1 publication

(1 citation statement)

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“…The antagonistic activity of P. fluorescens on A. flavus was tested by dual culture technique [14]. The toxigenic strain of A. flavus, AFT5b identified in our studies was used in the present study [15]. PGPR isolates were streaked at one side of Petri dish (one cm away from the edge) containing PDA.…”

Section: Dual Culture Studiesmentioning

confidence: 99%

Pseudomonas fluorescens Pf7: A Potential Biocontrol Agent against Aspergillus flavus Induced Aflatoxin Contamination in Groundnut

Teja

Kumar

Sudini

2019

AIR

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Aflatoxin contamination is a qualitative problem in groundnut (Arachis hypogaea L.) occurring at both pre-and post-harvest stages. These aflatoxins are secondary metabolites produced by Aspergillus flavus and A. parasiticus and have carcinogenic, hepatotoxic, teratogenic and immuno-suppressive effects. Use of plant growth-promoting rhizobacteria (PGPR) is a viable and sustainable option in managing aflatoxin problem in groundnut. Our present study is aimed at identifying a plant growth-promoting rhizobacteria (PGPR) strain with superior antagonistic abilities on A. flavus infection, aflatoxin contamination and to determine its mode of action. Ten native P. fluorescens isolates were isolated from groundnut rhizosphere and screened against A. flavus by dual culture and in vitro seed colonization (IVSC) assays. In dual culture and IVSC studies, Pf7 exhibited higher degree of antagonism on A. flavus (54% inhibition), inhibited its colonization and reduced aflatoxin contamination (27.8 µg kg-1) in kernels.

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Section: Dual Culture Studiesmentioning

confidence: 99%