Detection of Toxoplasma gondii infection in domestic rabbits by using multiple techniques

Aghwan, S. S.; Al-Taee, A. F.; Suliman, Esam

doi:10.33899/ijvs.2010.5594

Cited by 6 publications

(2 citation statements)

References 14 publications

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“…Humoral immunity (H.I.) and cell-mediated immunity (C.M.I) will be activated against T. gondii that infect intracellular and may pass through extracellular space to find new host cells (Aghwan et al, 2010). serum level of (CD4 & IFNγ,) in patients of toxoplasmosis, so increases or decreases during or after infection.…”

Section: Introductionmentioning

confidence: 99%

Toxoplasmosis and Relation with Some Immunological Markers for Aborted Women in Thi-Qar Province

2023

JPTCP

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Background: Toxoplasma gondii is a major cause of abortion in pregnant women. Toxoplasmosis infection in the mother during pregnancy is frequently associated with transplacental transmission of the parasite to the fetus. The purpose of this study was to determine the serum level concentrations of (CD4 and IFN-)in patients with toxoplasmosis and control groups, aims of this study determine the serum level concentration of (CD4, IFNγ) in patients with toxoplasmosis and control groups.and compare the different concentrations of (CD4, IFN) in acute, chronic, and control groups.e310

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Section: Introductionmentioning

confidence: 99%

Toxoplasmosis and Relation with Some Immunological Markers for Aborted Women in Thi-Qar Province

2023

JPTCP

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“…Therfore, toxoplasmosis causes reproductive failure in sheep (5). For the detection of T. gondii there are different techniques used for this purpose .For example directl methods like preparing of impression smears from infected organs, histological examination and indirect method by serological methodes like latex agglutination test (LAT), modiffed agglutination test (MAT), ELISA (6,7). Several PCR-based methods and Real-Time PCR quantitative methods have been developed for the detection of T. gondii using various samples like blood and tissue biopsy (8 ).…”

Section: Introductionmentioning

confidence: 99%

Comparison the efficiency of different techniques for the diagnosis of Toxoplasma gondii infection in slaughtered ewes

Aghwan¹,

Al-Bakri²,

Albaqqal³

2021

IJVS

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Toxoplasma gondii is one of the most common parasitic infections of human and other warm-blooded animals causes toxoplasmosis. In the present study a total of 50 uterus samples collected from slaughtered ewes were investigated for detection of T. gondii. Several techniques have been used to diagnose the infection with this parasite. Firstly, the impression smears staining methods used for the all samples using giemsa stain. Secondly, uses of direct fluorescence technique by acridine orange method for staining the impression smears of the uteri. As well as the histological section technique was used to determine the developmental growth stages of the parasite of all uterus samples and finally the serological method by latex agglutination test was used for the detection of antibodies of parasite. The results showed that detection of T. gondii using these four methods was 100, 80, 80 and 50%, respectively. It was concluded that the impression smears of the uterus staining with Giemsa stain was more readily, effectively and efficiently, followed by the direct immunofluorescence technique and histological section stained with hematoxylin and eosin stain technique, and finally the serological method.

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Serological survey for antibodies to <i>Encephalitozoon cuniculi</i> and <i>Toxoplasma gondii</i> in pet rabbits in eastern coastal areas of China

Wang

Qin

Xiu-guo³

et al. 2022

J. Vet. Med. Sci.

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Encephalitozoon cuniculi (E. cuniculi) is a microsporidian parasite commonly detected in rabbits and can infect humans and cause encephalitozoonosis. And Toxoplasma gondii is a prevalent parasite distributed worldwide and can infect almost all warm-blooded animals, including humans. The aim of the current study was to investigate the seroprevalence of E. cuniculi and Toxoplasma gondii, and risk factors of infection in pet rabbits reared in eastern coastal areas of China (Tianjin, Shandong, Jiangsu, Zhejiang, Shanghai and Fujian). Total 222 blood samples of pet rabbits were collected from local veterinary hospitals. The seropositivity rates of E. cuniculi were 16.22% (36/222) according to an Enzyme-linked immunosorbent assay (ELISA). Female pet rabbits was significantly higher than that in males (P=0.002), Zhejiang were markedly higher than those in Jiangsu and Shanghai (P=0.017, P=0.022), and cross-breed rabbits were dramatically higher than those in Chinchilla, New Zealand white, Rex (P=0.02, P=0.006, P=0.008). The seroprevalence of T. gondii was 13.06% (29/222) by the method of ELISA. The seroprevalence in Zhejiang was significantly higher than that in Shanghai (P=0.017). No difference in seroprevalence was detected with respect to the gender, age, species, health status, or season. These findings show that E. cuniculi and T. gondii are present and spread in pet rabbits. Therefore, pet rabbits should be considered as an important reservoir of encephalitozoonosis for humans and maybe important implication for public health in eastern coastal areas of China.

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Detection of Toxoplasma gondii infection in domestic rabbits by using multiple techniques

Cited by 6 publications

References 14 publications

Toxoplasmosis and Relation with Some Immunological Markers for Aborted Women in Thi-Qar Province

Toxoplasmosis and Relation with Some Immunological Markers for Aborted Women in Thi-Qar Province

Comparison the efficiency of different techniques for the diagnosis of Toxoplasma gondii infection in slaughtered ewes

Serological survey for antibodies to <i>Encephalitozoon cuniculi</i> and <i>Toxoplasma gondii</i> in pet rabbits in eastern coastal areas of China

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