Detection of Tuberculosis in HIV Co-infected Individuals: Use of Multiple ELISA Responses to 38kDa, Lipoarabinomannan and ESAT– 6 of M. tuberculosis

Gutlapalli, Ravi; Sykam, Aparna; Tenali, Sandeep P.; Chandran, Priscilla; Suneetha, Sujai; Suneetha, Lavanya M.

doi:10.7860/jcdr/2016/16559.7322

Cited by 10 publications

(9 citation statements)

References 29 publications

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“…We assessed the 8 antigens found to be useful for TB screening in Uganda (Ag85A, Ag85B, Ag85C, Rv0934-P38, Rv3881, Rv3841-BfrB, Rv3873, and Rv2878c) [13]. In addition, we included 4 antigens (ESAT-6, CFP-10, Rv1980-MPT64, and Rv2031-HSPX) that were in the first or second priority tier in Pakistan and are wellknown targets for TB diagnosis [21][22][23][24].…”

Section: Multiplex Microbead Immunoassaymentioning

confidence: 99%

Evaluation of multi-antigen serological screening for active tuberculosis among people living with HIV

et al. 2020

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Better triage tests for screening tuberculosis (TB) disease are needed for people living with HIV (PLHIV). We performed the first evaluation of a previously-validated 8-antigen serological panel to screen PLHIV for pulmonary TB in Kampala, Uganda. We selected a random 1:1 sample with and without TB (defined by sputum culture) from a cohort of PLHIV initiating antiretroviral therapy. We used a multiplex microbead immunoassay and an ensemble machine learning classifier to determine the area under the receiver operating characteristic curve (AUC) for Ag85A, Ag85B, Ag85C, Rv0934-P38, Rv3881, Rv3841-BfrB, Rv3873, and Rv2878c. We then assessed the performance with the addition of four TB-specific antigens ESAT-6, CFP-10, Rv1980-MPT64, and Rv2031-HSPX, and every antigen combination. Of 262 participants (median CD4 cell-count 152 cells/μL [IQR 65-279]), 138 (53%) had cultureconfirmed TB. The 8-antigen panel had an AUC of 0.53 (95% CI 0.40-0.66), and the additional 4 antigens did not improve performance (AUC 0.51, 95% CI 0.39-0.64). When sensitivity was restricted to �90% for the 8-and 12-antigen panel, specificity was 2.2% (95% CI 0-17.7%) and 8.1% (95% CI 0-23.9%), respectively. A three-antigen combination (Rv0934-P38, Ag85A, and Rv2031-HSPX) outperformed both panels, with an AUC of 0.60 (95% CI 0.48-0.73), 90% sensitivity (95% CI 78.2-96.7%) and 29.7% specificity (95% CI 15.9-47%). The multi-antigen panels did not achieve the target accuracy for a TB triage test among PLHIV. We identified a new combination that improved performance for TB screening in an HIV-positive sample compared to an existing serological panel in Uganda, and suggests an approach to identify novel antigen combinations specifically for screening TB in PLHIV.

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Section: Multiplex Microbead Immunoassaymentioning

confidence: 99%

Evaluation of multi-antigen serological screening for active tuberculosis among people living with HIV

et al. 2020

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“…At present, the most common methods of virus testing in clinical laboratory settings include as following: serological diagnosis based on antigen or antibody such as ELISA [2][3][4], and nucleic acid test methods including PCR [5], RT-PCR [6,7], real-time PCR [8][9][10][11][12][13], and molecular hybridization [14][15][16], etc. However, there may be different test results when different kits or test methods were used.…”

Section: Discussionmentioning

confidence: 99%

Attention to the problem of the inaccurate testing caused by virus mutation strains in clinical laboratory settings

Yulu¹

2017

Glob J Clin Virol

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“…MTBC species secrete a variety of antigens with potential diagnostic signi cance [11][12][13] . 38KDa protein is a membrane protein, closely related to the transport of phosphate.…”

Section: Introductionmentioning

confidence: 99%

Application value of tissue tuberculosis antigen combined with Xpert MTB/RIF detection in differential diagnoses of intestinal tuberculosis and Crohn’s disease

Fei

Zhou

Luo

et al. 2020

Preprint

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Background: The purpose of this study was to examine the value of Xpert MTB/RIF assay and detection of additional Mycobacterium tuberculosis complex (MTBC) species antigens from intestinal tissue samples in differentiating intestinal tuberculosis (ITB) from Crohn’s disease (CD).Methods: Several clinical specimens of intestinal tissue obtained by either endoscopic biopsy or surgical excision were used for mycobacteriologic solid cultures ,Xpert MTB/RIF assays , immunohistochemistry, and histological examinations. Four antigens (38KDa, ESAT-6, MPT64, and Ag85 complex) of MTBC in the intestinal tissue were detected by immunohistochemical analysis.Results: The study included 42 patients with ITB and 46 with CD. Perianal lesions and longitudinal ulcers were more common in patients with CD, while caseating granuloma and annular ulcers were more common in patients with ITB. The positive rate of MTBC detected by Xpert MTB/RIF in intestinal tissues of patients with ITB was 33.33%, which was significantly higher than that in patients with CD and that detected using acid-fast staining smears. It was also higher than that detected by tissue MTBC culture, but the difference was not statistically significant.The positive MPT64 expression rate in patients with ITB was 40.48%, which was significantly higher than that observed in patients with CD. The sensitivity of parallelly combined detection of tuberculosis protein MPT64 and Xpert MTB/RIF in diagnosing ITB was 50.0%.Conclusions: The detection of Xpert MTB/RIF in intestinal tissue is a rapid and useful method for establishing an early diagnosis of ITB. The detection of MTBC using Xpert MTB/RIF and MPT64 antigen in intestinal tissues has a definitive value in the differential diagnosis ofITB and CD. The combination of these two methods can improve the detection sensitivity.

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Detection of Tuberculosis in HIV Co-infected Individuals: Use of Multiple ELISA Responses to 38kDa, Lipoarabinomannan and ESAT– 6 of M. tuberculosis

Cited by 10 publications

References 29 publications

Evaluation of multi-antigen serological screening for active tuberculosis among people living with HIV

Evaluation of multi-antigen serological screening for active tuberculosis among people living with HIV

Attention to the problem of the inaccurate testing caused by virus mutation strains in clinical laboratory settings

Application value of tissue tuberculosis antigen combined with Xpert MTB/RIF detection in differential diagnoses of intestinal tuberculosis and Crohn’s disease

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