Detection of tumor marker in blood serum using antibody-modified field effect transistor with optimized BSA blocking

Hideshima, Sho; Sato, Ryosuke; Inoue, Shuichiro; Kuroiwa, Shigeki; Osaka, Tetsuya

doi:10.1016/j.snb.2011.10.001

Cited by 73 publications

(50 citation statements)

References 26 publications

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“…BSA is of importance for blocking nonspecific adsorption. If no blocking agent, undesired molecules can either be absorbed or sink on the sensing surface, and as a result, it causes signal fluctuation [20]. The sensor was immediately used after the cycle of antibody modification.…”

Section: Sensors and Functionalization For Afp Detectionmentioning

confidence: 99%

Shrink-induced graphene sensor for alpha-fetoprotein detection with low-cost self-assembly and label-free assay

2017

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Combination of shrink induced nano-composites technique and layer-by-layer (LbL) self-assembled graphene challenges controlling surface morphology. Adjusting shrink temperature achieves tunability on graphene surface morphology on shape memory polymers, and it promises to be an alternative in fields of highsurface-area conductors and molecular detection. In this study, self-assembled graphene on a shrink polymer substrate exhibits nanowrinkles after heating. Induced nanowrinkles on graphene with different shrink temperature shows distinct surface roughness and wettability. As a result, it becomes more hydrophilic with higher shrink temperatures. The tunable wettability promises to be utilized in, for example, microfluidic devices. The graphene on shrink polymer also exhibits capability of being used in sensing applications for pH and alphafetoprotein (AFP) detection with advantages of label free and low cost, due to self-assembly technique, easy functionalization, and antigen-antibody reaction on graphene surface. The detection limit of AFP detection is down to 1 pg/mL, and therefore the sensor also has a significant potential for biosensing as it relies on low-cost self-assembly and label-free assay.

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Section: Sensors and Functionalization For Afp Detectionmentioning

confidence: 99%

Shrink-induced graphene sensor for alpha-fetoprotein detection with low-cost self-assembly and label-free assay

2017

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“…(8,9,15,16) In comparison, the biosensor based on a field effect device has attracted great attention for labelfree detection of biomolecules. (9,(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25) The other label-free methods such as surface plasmon resonance (SPR) (26,27) and quartz crystal microbalance (QCM) (28,29) require lengthy steps and complex handling. In addition, immunochromatography and chromatography are quick and simple, however, they require expensive instrumentation.…”

Section: Introductionmentioning

confidence: 99%

Multianalyte Detection of Cancer Biomarkers in Human Serum Using a Label-Free Field Effect Transistor Biosensor

Cheng

Hideshima³

et al. 2018

Sensors and Materials

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Recent statistics show that the incidence and mortality of cancer are on the rise. Among all types of cancer, lung cancer and liver cancer are the most prevalent. We have many biomarkers for these two cancers, among which the cytokeratin fragment 21-1 (CYFRA 21-1) and α-fetoprotein (AFP) are the most common and widely used. Among the various detection methods, the field effect transistor (FET) biosensor is one of the most attractive approaches, providing a label-free, fast, and low-cost electrical detection of biomarkers with high specificity and sensitivity. In previous work, we applied biomarkers for a single cancer type. With the detection of CYFRA 21-1 and neuron-specific enolase (NSE), lung cancer was differentiated in the early stages. Compared with the early work, the biomarkers for the two cancer types, CYFRA 21-1 for lung cancer and AFP for liver cancer, were prepared in this work. The detection of CYFRA 21-1 and AFP in human serum was achieved at the same time with the limits of detection of 1 and 10 ng mL −1 , while the cut-off values were 4 and 10 ng mL −1 . The method featured short analytical time, small sample volume, and low cost. With its good selectivity and appropriate sensitivity, clinical application will become popular in the future.

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“…On the other hand, using short receptors alone may not be sufficient to achieve appropriate detection limits, as a large part of the analyte may be still screened by the electrolyte ions due to very short Debye length in high ionic strength solutions (<1 nm). As a result, additional strategies have been investigated to address this challenge [9][10][11][12]. Unfortunately, most of these approaches require sample dilution, multiple washing steps or elaborate labelling schemes.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Immunodetection in High Ionic Strength Solutions Using Carbon Nanotube Transistors with Nanobody Receptors

Filipiak

Rother

Andoy

et al. 2017

Proceedings of Eurosensors 2017, Paris, France, 3–6 September 2017

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Nanomaterial-based field-effect transistors (FETs) have been proposed for real-time, label-free detection of various biological species. However, screening of the analyte charge by electrolyte ions (Debye screening) has so far limited their use in physiological samples. Here, this challenge is overcome by combining FETs based on single-walled semiconducting carbon nanotube networks (SWCNTs) with a novel surface functionalization comprising: (1) short nanobody receptors, and (2) a polyethylene glycol layer (PEG). Nanobodies are stable, easy-to-produce, short biological receptors (~2-4 nm) that enable analyte binding closer to the sensor surface. The addition of PEG enhances the signal in high ionic strength environment. Using green fluorescent protein (GFP) as a model antigen, high selectivity and sub-picomolar detection limit with a dynamic range exceeding 4 orders of magnitude is demonstrated in physiological solutions. The presented immunoassay is fast, label-free, does not require any sample pre-treatment or washing steps.

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Detection of tumor marker in blood serum using antibody-modified field effect transistor with optimized BSA blocking

Cited by 73 publications

References 26 publications

Shrink-induced graphene sensor for alpha-fetoprotein detection with low-cost self-assembly and label-free assay

Shrink-induced graphene sensor for alpha-fetoprotein detection with low-cost self-assembly and label-free assay

Multianalyte Detection of Cancer Biomarkers in Human Serum Using a Label-Free Field Effect Transistor Biosensor

Label-Free Immunodetection in High Ionic Strength Solutions Using Carbon Nanotube Transistors with Nanobody Receptors

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