Detection of tumor suppressor protein p53 with special emphasis on biosensors: A review

Deepa, .; Pundir, Shikha; Pundir, C.S.

doi:10.1016/j.ab.2019.113473

Cited by 31 publications

(17 citation statements)

References 57 publications

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“…Since p53 was first identified, numerous studies have indicated that p53 plays essential roles in biological processes [47]. Our laboratory has focused on p53 regulation of radiation-induced injury over the past decade, and this study was performed to assess the potential interactions of mRNA and metabolites regulated by p53 during the cellular response to radiation.…”

Section: Discussionmentioning

confidence: 99%

Integrated analysis of transcriptomic and metabolomic profiling reveal the p53 associated pathways underlying the response to ionizing radiation in HBE cells

Huang

Liu

et al. 2020

Cell Biosci

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Background: Radiation damage to normal tissues is a serious concern. P53 is a well-known transcription factor which is closely associated with radiation-induced cell damage. Increasing evidence has indicated that regulation of metabolism by p53 represents a reviving mechanism vital to protect cell survival. We aimed to explore the interactions of radiation-induced transcripts with the cellular metabolism regulated by p53.Methods: Human bronchial epithelial (HBE) cell line was used to knockout p53 using CRISPR/cas9. Transcriptomic analysis was conducted by microarray and metabolomic analysis was conducted by GC-MS. Integrative omics was performed using MetaboAnalyst.Results: 326 mRNAs showed significantly altered expression in HBE p53-/-cells post-radiation, of which 269 were upregulated and 57 were downregulated. A total of 147 metabolites were altered, including 45 that increased and 102 that decreased. By integrated analysis of both omic data, we found that in response to radiation insult, nitrogen metabolism, glutathione metabolism, arachidonic acid metabolism, and glycolysis or gluconeogenesis may be dysregulated due to p53. Conclusions:Our study provided a pilot comprehensive view of the metabolism regulated by p53 in response to radiation exposure. Detailed evaluation of these important p53-regulated metabolic pathways, including their roles in the response to radiation of cells, is essential to elucidate the molecular mechanisms of radiation-induced damage.

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Section: Discussionmentioning

confidence: 99%

Integrated analysis of transcriptomic and metabolomic profiling reveal the p53 associated pathways underlying the response to ionizing radiation in HBE cells

Huang

Liu

et al. 2020

Cell Biosci

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“…Considering their vital impact on metabolism and life, the analysis of proteins is of great interest for many purposes. The screening of enzymes, peptides, hormones, and cell surface proteins provides a useful methodology for monitoring disease incidence . Most of the protein biosensors have been attempted to be used for cancer diagnosis purposes.…”

Section: Homogeneous Electrochemiluminescence Biosensorsmentioning

confidence: 99%

Homogeneous Electrochemiluminescence in the Sensors Game: What Have We Learned from Past Experiments?

et al. 2021

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“…Biosensors for protein determination can be involved in detecting diseases and pathogenic microorganisms such as viruses, bacteria, parasites, and fungi, and even in the identification of antimicrobial resistance patterns (24)(25)(26)(27)(28)(29)(30). Some of the most important proteins detected using biosensors are the p53 protein, expressed in more than 90% of cancers (31,32), the Tau protein, useful for the diagnosis of Alzheimer's (33), and C-reactive proteins, involved in inflammation processes (34). Recently, the monitoring of antigenic proteins of SARS-CoV-2 has been reported, which could be implemented for the rapid detection of Covid-19 (35).…”

Section: Biosensors As Medical Devicesmentioning

confidence: 99%

Nucleic acid-based biosensors: analytical devices for prevention, diagnosis and treatment of diseases

Barbosa

Cepeda

León-Torres

et al. 2021

Vitae

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BACKGROUND : Biosensing techniques have been the subject of exponentially increasing interest due to their performance advantages such as high selectivity and sensitivity, easy operation, low cost, short analysis time, simple sample preparation, and real-time detection. Biosensors have been developed by integrating the unique specificity of biological reactions and the high sensitivity of physical sensors. Therefore, there has been a broad scope of applications for biosensing techniques, and nowadays, they are ubiquitous in different areas of environmental, healthcare, and food safety. Biosensors have been used for environmental studies, detecting and quantifying pollutants in water, air, and soil. Biosensors also showed great potential for developing analytical tools with countless applications in diagnosing, preventing, and treating diseases, mainly by detecting biomarkers. Biosensors as a medical device can identify nucleic acids, proteins, peptides, metabolites, etc.; these analytes may be biomarkers associated with the disease status. Bacterial food contamination is considered a worldwide public health issue; biosensor-based analytical techniques can identify the presence or absence of pathogenic agents in food. OBJECTIVES: The present review aims to establish state-of-the-art, comprising the recent advances in the use of nucleic acid-based biosensors and their novel application for the detection of nucleic acids. Emphasis will be given to the performance characteristics, advantages, and challenges. Additionally, food safety applications of nucleic acid-based biosensors will be discussed. METHODS: Recent research articles related to nucleic acid-based biosensors, biosensors for detecting nucleic acids, biosensors and food safety, and biosensors in environmental monitoring were reviewed. Also, biosensing platforms associated with the clinical diagnosis and food industry were included. RESULTS: It is possible to appreciate that multiple applications of nucleic acid-based biosensors have been reported in the diagnosis, prevention, and treatment of diseases, as well as to identify foodborne pathogenic bacteria. The use of PNA and aptamers opens the possibility of developing new biometric tools with better analytical properties. CONCLUSIONS: Biosensors could be considered the most important tool for preventing, treating, and monitoring diseases that significantly impact human health. The aptamers have advantages as biorecognition elements due to the structural conformation, hybridization capacity, robustness, stability, and lower costs. It is necessary to implement biosensors in situ to identify analytes with high selectivity and lower detection limits.

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Detection of tumor suppressor protein p53 with special emphasis on biosensors: A review

Cited by 31 publications

References 57 publications

Integrated analysis of transcriptomic and metabolomic profiling reveal the p53 associated pathways underlying the response to ionizing radiation in HBE cells

Integrated analysis of transcriptomic and metabolomic profiling reveal the p53 associated pathways underlying the response to ionizing radiation in HBE cells

Homogeneous Electrochemiluminescence in the Sensors Game: What Have We Learned from Past Experiments?

Nucleic acid-based biosensors: analytical devices for prevention, diagnosis and treatment of diseases

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