Detection of tyrosine and monitoring tyrosinase activity using an enzyme cascade-triggered colorimetric reaction

Chen, Huei Yu; Yeh, Yi Chun

doi:10.1039/d0ra05581f

Cited by 28 publications

(8 citation statements)

References 30 publications

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“…In-vivo samples/tissues with excess initial Tyrosine concentrations could affect Zn 2+ imaging by AT2, therefore, careful monitoring on primary Tyrosine concentrations was mandatory. Finally, the use and performance of AT2-probe towards Zn 2+ and Tyrosine detection were reliable and comparable to earlier reports [38,[42][43][44][59][60][61][62][63][64][65][66][67][68][69][70][71][72] in terms of linear range, LODs and applications as displayed in Tables S3 and S4 (SI). Note that AT2-based sequential detection of Zn 2+ and Tyrosine was considered as exceptional in terms of one-pot synthesize, linear range nanomolar LODs, and bioimaging applications.…”

Section: Mtt Assay and Bioimagingsupporting

confidence: 86%

An AIEE Active Anthracene-Based Nanoprobe for Zn2+ and Tyrosine Detection Validated by Bioimaging Studies

et al. 2022

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Novel anthracene-based Schiff base derivative (4-(anthracen-9-ylmethylene) amino)-5-phenyl-4H-1,2,4-triazole-3-thiol; AT2) is synthesized and utilized as an aggregation-induced emission-enhancement (AIEE) active probe to detect Zn2+ and Tyrosine. Ultraviolet-visible absorption/photoluminescence (UV-vis/PL) spectroscopy studies on the AIEE property of AT2 (in ethanol) with increasing water fractions (fw: 0–97.5%) confirm the J-type aggregation. Excellent sensor selectivity of AT2 to Zn2+ and its reversibility with Tyrosine are demonstrated with PL interrogations. 2:1 and 1:1 stoichiometry and binding sites of AT2-Zn2+ and Tyrosine-Zn2+ complexes are elucidated from Job plots, HR-mass, and 1H-NMR results. Nanomolar-level detection limits (LODs) of Zn2+ (179 nM) and Tyrosine (667 nM) and association constants (Kas) of 2.28 × 10−6 M−2 (for AT2-Zn2+) and 1.39 × 10−7 M−1 (for Tyrosine-Zn2+) are determined from standard deviation and linear fittings. Nanofiber formation in AIEE and aggregated/dispersed nanoparticles in the presence of the Zn2+/Tyrosine are supported by scanning-electron microscope (SEM), transmission-electron microscope (TEM), atomic-force microscope (AFM), and dynamic-light scattering (DLS) investigations. Density-functional theory (DFT) studies confirm an “On-Off” twisted intramolecular charge transfer/photo-induced electron transfer (TICT/PET) and “On-Off-On” PET mechanisms for AIEE and sensors, respectively. B16-F10 cellular and zebrafish imaging are conducted to support the applications of AIEE and sensors.

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Section: Mtt Assay and Bioimagingsupporting

confidence: 86%

An AIEE Active Anthracene-Based Nanoprobe for Zn2+ and Tyrosine Detection Validated by Bioimaging Studies

et al. 2022

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“…Therefore, an economically efficient means of analyzing the levels of L-tyrosine is highly needed to ensure the wellbeing of living systems. To date, various sophisticated analytical methods, including chromatography, electrophoresis, mass spectral analysis and electrochemical methods, have been employed for the recognition of L-tyrosine [ 11 , 12 ]. Conversely, the costly instrumentation, time-consuming sample processing and the need for skilled professional experts have restricted the widespread use of these available methodologies in the large-scale sensing of L-tyrosine.…”

Section: Introductionmentioning

confidence: 99%

Coconut Carbon Dots: Progressive Large-Scale Synthesis, Detailed Biological Activities and Smart Sensing Aptitudes towards Tyrosine

et al. 2022

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In the recent era, carbon dots (C-dots) have been extensively considered as a potential tool in drug delivery analysis. However, there have been fewer reports in the literature on their application in the sensing of amino acids. As part of our ongoing research on coconut-husk-derived C-dots, we synthesized C-dots under different temperature conditions and utilized them in the field of amino acid sensing and found them to be highly selective and sensitive towards tyrosine. The detailed characterization of the prepared C-dots was carried out. The developed C-dots exhibit good values of quantum yield. BSA, HSA and glutamic acid were utilized to explore the binding efficiency of C-dots with biologically active components. Hemolysis, blood clotting index activity and cell viability assays using the prepared C-dots were evaluated and they were found to be biocompatible. Therefore, the C-dots described in this work have high potential to be utilized in the field of amino acid sensing, especially L-tyrosine. The limit of detection and the binding constant for the developed C-dots in the presence of tyrosine were found to be 0.96 nM and 296.38 nM−1, respectively. The efficiency of the developed C-dots was also investigated in the presence of various other amino acids and different water mediums in order to enhance the working scope of the developed sensors.

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“…Along with other amino acids, Tyr also plays an important role in the development of Alzheimer's and Parkinson's disease [18]. Thus, point of care (POC) measurement of this valuable aromatic biomarker present an important opportunity for early diagnosis and monitoring of metabolic or neurodegenerative diseases [1,18,20].…”

Section: Introductionmentioning

confidence: 99%

Disposable Electrochemical Sensor Using Graphene Oxide–Chitosan Modified Carbon-Based Electrodes for the Detection of Tyrosine

2021

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Detection of tyrosine and monitoring tyrosinase activity using an enzyme cascade-triggered colorimetric reaction

Abstract: We developed an enzyme cascade-triggered colorimetric reaction for the detection of tyrosine, based on the formation of yellow pigment (betalamic acid) and red fluorometric betaxanthin.

Cited by 28 publications

References 30 publications

An AIEE Active Anthracene-Based Nanoprobe for Zn2+ and Tyrosine Detection Validated by Bioimaging Studies

An AIEE Active Anthracene-Based Nanoprobe for Zn2+ and Tyrosine Detection Validated by Bioimaging Studies

Coconut Carbon Dots: Progressive Large-Scale Synthesis, Detailed Biological Activities and Smart Sensing Aptitudes towards Tyrosine

Disposable Electrochemical Sensor Using Graphene Oxide–Chitosan Modified Carbon-Based Electrodes for the Detection of Tyrosine

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