Detection of up-regulated genes in thrombin-stimulated human umbilical vein endothelial cells

Okada, Makoto; Suzuki, Kunihiro; Takada, Kunio; Nakashima, Masahiro; Nakanishi, Takashi; Shinohara, Tadashi

doi:10.1016/j.thromres.2005.11.008

Cited by 35 publications

(25 citation statements)

References 29 publications

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“…It has been reported that endothelial cells in culture express CCL20 upon thrombin stimulation [52]. CCL20 seems to be implicated in endothelial cell-lymphocyte interaction through CCR6 [53].…”

Section: Resultsmentioning

confidence: 99%

Gene signatures in wound tissue as evidenced by molecular profiling in the chick embryo model

et al. 2010

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BackgroundModern functional genomic approaches may help to better understand the molecular events involved in tissue morphogenesis and to identify molecular signatures and pathways. We have recently applied transcriptomic profiling to evidence molecular signatures in the development of the normal chicken chorioallantoic membrane (CAM) and in tumor engrafted on the CAM. We have now extended our studies by performing a transcriptome analysis in the "wound model" of the chicken CAM, which is another relevant model of tissue morphogenesis.ResultsTo induce granulation tissue (GT) formation, we performed wounding of the chicken CAM and compared gene expression to normal CAM at the same stage of development. Matched control samples from the same individual were used. We observed a total of 282 genes up-regulated and 44 genes down-regulated assuming a false-discovery rate at 5% and a fold change > 2. Furthermore, bioinformatics analysis lead to the identification of several categories that are associated to organismal injury, tissue morphology, cellular movement, inflammatory disease, development and immune system. Endothelial cell data filtering leads to the identification of several new genes with an endothelial cell signature.ConclusionsThe chick chorioallantoic wound model allows the identification of gene signatures and pathways involved in GT formation and neoangiogenesis. This may constitute a fertile ground for further studies.

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Section: Resultsmentioning

confidence: 99%

Gene signatures in wound tissue as evidenced by molecular profiling in the chick embryo model

et al. 2010

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“…The chemokine CCL20 was originally described to be constitutively expressed in liver tissue [8]. Studies from others, however, showed that CCL20 is also inducible in a variety of other cell types such as PBMCs [29], PMNs [25], and ECs [19].…”

Section: Discussionmentioning

confidence: 99%

The Anti-Inflammatory Effect of Low-Dose Radiation Therapy Involves a Diminished CCL20 Chemokine Expression and Granulocyte/Endothelial Cell Adhesion

et al. 2008

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“…Glyceraldeyde-3-phosphate dehydrogenase (GAPDH) was used as the selected housekeeping gene for normalization. The primer sequences for GAPDH, CXCL5, CXCL3 and CCL20 have been described previously [6,12,15]. Data were analyzed using Pfaffl method to calculate fold change of gene expression [16].…”

Section: Methodsmentioning

confidence: 99%

PAR1- and PAR2-induced innate immune markers are negatively regulated by PI3K/Akt signaling pathway in oral keratinocytes

Rohani

DiJulio

et al. 2010

BMC Immunol

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BackgroundProtease-Activated Receptors (PARs), members of G-protein-coupled receptors, are activated by proteolytic activity of various proteases. Activation of PAR1 and PAR2 triggers innate immune responses in human oral keratinocytes (HOKs), but the signaling pathways downstream of PAR activation in HOKs have not been clearly defined. In this study, we aimed to determine if PAR1- and PAR2-mediated signaling differs in the induction of innate immune markers CXCL3, CXCL5 and CCL20 via ERK, p38 and PI3K/Akt.ResultsOur data show the induction of innate immunity by PAR1 requires both p38 and ERK MAP kinases, while PAR2 prominently signals via p38. However, inhibition of PI3K enhances expression of innate immune markers predominantly via suppressing p38 phosphorylation signaled by PAR activation.ConclusionOur data indicate that proteases mediating PAR1 and PAR2 activation differentially signal via MAP kinase cascades. In addition, the production of chemokines induced by PAR1 and PAR2 is suppressed by PI3K/Akt, thus keeping the innate immune responses of HOK in balance. The results of our study provide a novel insight into signaling pathways involved in PAR activation.

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Detection of up-regulated genes in thrombin-stimulated human umbilical vein endothelial cells

Cited by 35 publications

References 29 publications

Gene signatures in wound tissue as evidenced by molecular profiling in the chick embryo model

Gene signatures in wound tissue as evidenced by molecular profiling in the chick embryo model

The Anti-Inflammatory Effect of Low-Dose Radiation Therapy Involves a Diminished CCL20 Chemokine Expression and Granulocyte/Endothelial Cell Adhesion

PAR1- and PAR2-induced innate immune markers are negatively regulated by PI3K/Akt signaling pathway in oral keratinocytes

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