Detection of various U and M chromosomes in wheat-Aegilops biuncialis hybrids and derivatives using fluorescence in situ hybridisation and molecular markers

Abstract

Abstract:The aim of the study was to select wheat-Aegilops biuncialis addition lines carrying Aegilops biuncialis chromosomes differing from those which were introgressed into the wheat-Ae. After FISH no hybridisation site was observed on the Ae. biuncialis chromosome pair using the pSc119.2 and Afa family repetitive DNA probes, so it was not possible to identify the Ae. biuncialis chromosome pair. However, the use of wheat SSR markers and the (GAA) n microsatellite DNA probe allowed it to be characterised more accurately. These new lines facilitate gene transfer from Ae. biuncialis into cultivated wheat and the selection of U and M genome-specific wheat SSR markers.

Characterization of a New Wheat-Aegilops biuncialis1Mb(1B) Substitution Line with Good Quality-associated HMW Glutenin Subunit

“…It is reported that the wheat-Ae. biuncialis disomic addition lines (DAL2U b , DAL2M b , DAL3U b , DAL3M b , DAL5U b , DAL6M b , and DAL7U b ) were created, and all of these addition lines had reduced fertility (Schneider et al 2005;Schneider and Molnár-Láng 2012). And a wheat-Ae.…”

“…Up-to-now, very few chromosome-specific molecular markers have been developed in Aegilops species (Gong et al 2014). Moreover, only 2 simple sequence repeat (SSR) markers GWM44 and GDM61 (Schneider and Molnár-Láng 2012) and some gene-based conserved orthologous set (COS) markers (Molnár et al 2013) were obtained in Ae. biuncialis.…”

“…And the species also has a high potential for improving the protein content and flour quality of the wheat grain . These important genes can be incorporated into common wheat through amphiploids, addition lines, substitution lines or translocation lines (Schneider et al 2005;Schneider and Molnár-Láng 2012;Farkas et al 2014).…”

Characterization of a new wheat-Aegilops biuncialis addition line conferring quality-associated HMW glutenin subunits

“…These results can be used in further research. were created, and all of these addition lines had reduced fertility compared with that of the parent (Schneider et al, 2005;Schneider and Molnár-Láng, 2012). In this study, a new wheat-A.…”

“…These useful genes can be incorporated into cultivated wheat by developing addition lines, substitution lines, or translocation lines (Schneider et al, 2005(Schneider et al, , 2008bMolnár et al, 2009;Schneider and Molnár-Láng, 2012).…”