Detection of Vibrio anguillarum and Vibrio alginolyticus by Singleplex and Duplex Loop-Mediated Isothermal Amplification (LAMP) Assays Targeted to groEL and fklB Genes

Siddique, Mahbubul Pratik; Jang, Won Je; Lee, Jong Min; Hasan, Tawheed; Kim, Chang Hoon; Kong, In Soo

doi:10.1007/s10123-019-00079-z

Cited by 14 publications

(7 citation statements)

References 30 publications

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“…However, Gro EL is a member of the molecular chaperon family Gro E system, together with gro ES [ 42 ]. The gro EL gene-based PCR assays have already been established by various researchers in the last few decades [ 24 , 26 , 43 , 44 ]and have proven to be powerful phylogenetic markers [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

groEL gene-based molecular detection and antibiogram profile of Riemerella anatipestifer from duck in Bangladesh

Hasan¹,

Bose²,

Aktar³

et al. 2022

J Adv Vet Anim Res

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Objectives: This study was designed to detect Riemerella anatipestifer through polymerase chain reaction (PCR) from duck farming areas of the Mymensingh and Sylhet divisions and to determine the antibiogram profile of the PCR-positive isolates using the disc diffusion method. Materials and Methods: Fifty two samples were collected, comprising clinically sick (32 ducks) and dead ducks (20). PCR confirmation was accomplished, and consistent findings were observed, employing R. anatipestifer groEL (271-bp) gene as appropriate molecular markers. For further clarification, see R. anatipestifer specific PCR assay (546-bp) and gyrB-based PCR (162-bp) were also done. The disc diffusion method was followed for the antibiotic susceptibility test of the iso¬lates against commonly used antibiotics. Results: A total of 21 samples, 8 from clinically sick birds and 13 from dead birds, showed positive results in both conventional and molecular assays out of 52 samples. High occurrences were found in oropharyngeal swabs from sick ducks and the liver and heart from dead ducks. Antibiotic susceptibility testing revealed that the isolates were 100% resistant to penicillin G, cefradine, streptomycin, neomycin, gentamycin, meropenem, and erythromycin, but 100% sensitive to cotrimoxazole, florfenicol, and levofloxacin. Conclusion: For diverse duck-populated areas in Bangladesh, this study shows the severity of R. anatipestifer infection among ducks.

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Section: Discussionmentioning

confidence: 99%

groEL gene-based molecular detection and antibiogram profile of Riemerella anatipestifer from duck in Bangladesh

Hasan¹,

Bose²,

Aktar³

et al. 2022

J Adv Vet Anim Res

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“…Hence, considerable research has been undertaken to elucidate its underlying pathogenic virulence mechanisms and develop rapid detection techniques and effective disease prevention strategies. Despite these efforts, not much is known regarding V. anguillarum especially compared with that of human pathogenic Vibrio spp., including V. cholerae, V. parahaemolyticus, and V. vulni cus [8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Homologous relationship between FabG involved in fatty acid biosynthesis and SDR on chromosome II in the multi-chromosome pathogen Vibrio anguillarum

Kim,

Park,

Rafquzzaman

et al. 2024

Preprint

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Vibrio anguillarum threatens fish and larval farming industries and human health worldwide. The fatty acids of cell membrane phospholipids are crucial to bacterial response to diverse environments and survival. Previously, short-chain dehydrogenase/reductase (SDR) was identified on chromosome II of the multichromosomal V. anguillarum. A comparison of SDR and the enzyme FabG-1b (encoded on chromosome I and responsible for the β-ketoacyl acyl carrier protein (ACP) reductase in fatty acid biosynthesis (FAS II)) showed that the amino acid sequence homology was only 33.2%; however, the core of functionality, which includes the NAD(P)-binding domain and the conserved region of the active site, the topologies predicted using sequence-based homology modeling, and the quaternary homotetramer-type structures showed a significant similarity. FabG-1b was specific to the substrates fluorinated and halogenated aliphatic ketones, aromatic ketones, and aromatic β-ketoesters and SDR toward non-fluorinated and non-halogenated aliphatic ketones, aromatic ketones, and non-aromatic β-ketoesters. This complementary catalytic efficiencies of the two enzymes on various substrates conclusively supports the hypothesis that the two enzymes are likely homologs. This is the first study to report potential paralogous enzymes FabG-1b and SDR in Vibrio. This information improves our understanding of bacterial FAS for establishing strategies to overcome infectious diseases caused by pathogenic strains and identify targets for developing new antibacterial agents.

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“…To address this issue, antibiotics are widely used, the abuse of which can lead to an increase in bacterial antibiotic resistance [8]. Rapid detection is essential to reduce the loss of infection with bacteria [9]. Therefore, a rapid, sensitive, and accurate method to detect V. alginolyticus is urgently required.…”

Section: Introductionmentioning

confidence: 99%

A Novel Method for Sensitive Detection of Vibrio alginolyticus Based on Aptamer and Hybridization Chain Reaction in Aquaculture

Zhao,

Luo,

Qiao

et al. 2023

Fishes

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In this study, we developed a novel method for the detection of Vibrio alginolyticus, utilizing the specific recognition of an aptamer for V. alginolyticus and signal amplification via hybridization chain reaction (HCR) and horseradish-peroxidase-conjugated streptavidin. The proposed HCR-based multivalent aptamer (multi-Apt) amplifier allows for sensitive detection of V. alginolyticus in a linear range from 10 to 107 CFU/mL. The linear equation is y = 747.5x + 126.2, R2 = 0.986, and the limit of detection (LOD) is 3 CFU/mL. Seawater and freshwater samples were utilized in the spike recovery experiment, yieldng a recovery rates ranging from 94.3% to 108.8%. The relative standard deviation (RSD) for all samples is below 6.73%. Taken together, the proposed method has great potential for application in monitoring of V. alginolyticus in aquaculture environments.

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Detection of Vibrio anguillarum and Vibrio alginolyticus by Singleplex and Duplex Loop-Mediated Isothermal Amplification (LAMP) Assays Targeted to groEL and fklB Genes

Cited by 14 publications

References 30 publications

groEL gene-based molecular detection and antibiogram profile of Riemerella anatipestifer from duck in Bangladesh

groEL gene-based molecular detection and antibiogram profile of Riemerella anatipestifer from duck in Bangladesh

Homologous relationship between FabG involved in fatty acid biosynthesis and SDR on chromosome II in the multi-chromosome pathogen Vibrio anguillarum

A Novel Method for Sensitive Detection of Vibrio alginolyticus Based on Aptamer and Hybridization Chain Reaction in Aquaculture

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