Detection of Vibrio campbellii and V. parahaemolyticus carrying full-length pirAB but only V. campbellii produces Pir  toxins

Vicente, Andrea; Taengphu, Suwimon; Hung, Armando; Mora, Chìo Marcela; Dong, Ha Thanh; Senapin, Saengchan

doi:10.1016/j.aquaculture.2019.734708

Cited by 14 publications

(8 citation statements)

References 31 publications

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“…Vibrios are prevalent bacterial pathogens in shrimp farming; however, these bacteria being part of the shrimp microbiota and being typically opportunistic pathogens. Some pathogenic strains can cause diseases, such as AHPND [66], because bear a plasmid that encodes the lethal toxins pirA and pirB [21, 25, 26], which operate in a binary manner and lead to severe hepatopancreas atrophy in shrimp [28, 29]. In this study, we evaluated the cellular and transcriptomic response of L. vannamei through exposing the shrimps to challenge with two strains of V. parahaemolyticus , including one non-pathogenic (VpN) and the other pathogenic (VpP) strain.…”

Section: Discussionmentioning

confidence: 99%

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Cellular and transcriptomic response to pathogenic and non-pathogenicVibrio parahaemolyticusstrains causing acute hepatopancreatic necrosis disease (AHPND) inLitopenaeus vannamei

López-Landavery,

Urquizo-Rosado,

Saavedra-Flores

et al. 2023

Preprint

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The shrimp industry has historically been affected by viral and bacterial diseases. One of the most recent emerging diseases is the Acute Hepatopancreatic Necrosis Disease (AHPND), which causes severe mortality. Despite its significance to both sanitation and economics, little is known about the molecular response of shrimp to this disease. Here, we present the cellular and transcriptomic responses ofLitopenaeus vannameiexposed to twoVibrio parahaemolyticusstrains for 98h, wherein one is non-pathogenic (VpN) and the other causes AHPND (VpP). Exposure to VpN strain resulted in minor alterations in hepatopancreas morphology, including reductions in the size of R and B cells as well as detachments of small epithelial cells from 72 h onwards. On the other hand, exposure to VpP strain is characterized by acute detachment of epithelial cell from the hepatopancreatic tubules and infiltration of hemocytes in the inter-tubular spaces. At the end of exposure, RNA-Seq analysis revealed functional enrichment in biological processes, such as thetoll3receptor signaling pathway, apoptotic processes, and production of molecular mediators involved in the inflammatory response of shrimp exposed to VpN treatment. The biological processes identified in the VpP treatment include superoxide anion metabolism, innate immune response, antimicrobial humoral response, andtoll3receptor signaling pathway. Furthermore, KEGG enrichment analysis revealed metabolic pathways associated with survival, cell adhesion, and reactive oxygen species, among others, for shrimp that were exposed to VpP. Our study proves the differential immune responses to two strains ofV. parahaemolyticus,one pathogenic and the other nonpathogenic, enlarges our knowledge on the evolution of AHPND inL. vannamei, and uncovers unique perspectives on establishing genomic resources that may function as a groundwork for detecting probable molecular markers linked to the immune system in shrimp.

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Section: Discussionmentioning

confidence: 99%

“…The etiological agent responsible for AHPND, known as VpAHPND, comprises a group of Gram-negative bacteria belonging to the genus Vibrio that naturally exist in marine and river ecosystems [21]. Their distribution largely relies on temperature, salinity, and nutrients [22, 23].…”

Section: Introductionmentioning

confidence: 99%

Cellular and transcriptomic response to pathogenic and non-pathogenicVibrio parahaemolyticusstrains causing acute hepatopancreatic necrosis disease (AHPND) inLitopenaeus vannamei

López-Landavery,

Urquizo-Rosado,

Saavedra-Flores

et al. 2023

Preprint

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“…campbellii was the dominant member across all samples, primarily driven by its over-representation in the intestinal tissue. V. campbellii has been implicated in shrimp diseases ( Haldar et al, 2011 ; Vicente et al, 2020 ), but little is known about its associations with oysters. In addition, V. rotiferianus, V. owensii and V. harveyi were the most dominant members recovered from oysters and these were present in outer and inner shell swabs, haemolymph, digestive tissue, large intestine and seawater, but not heart, gill nor mantle tissue.…”

Section: Discussionmentioning

confidence: 99%

Pearl Oyster Bacterial Community Structure Is Governed by Location and Tissue-Type, but Vibrio Species Are Shared Among Oyster Tissues

et al. 2021

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Diseases of bivalves of aquacultural importance, including the valuable Australian silver-lipped pearl oyster (Pinctada maxima), have been increasing in frequency and severity. The bivalve microbiome is linked to health and disease dynamics, particularly in oysters, with putative pathogens within the Vibrio genus commonly implicated in oyster diseases. Previous studies have been biased toward the Pacific oyster because of its global dominance in oyster aquaculture, while much less is known about the microbiome of P. maxima. We sought to address this knowledge gap by characterizing the P. maxima bacterial community, and we hypothesized that bacterial community composition, and specifically the occurrence of Vibrio, will vary according to the sampled microenvironment. We also predicted that the inside shell swab bacterial composition could represent a source of microbial spillover biofilm into the solid pearl oyster tissues, thus providing a useful predictive sampling environment. We found that there was significant heterogeneity in bacterial composition between different pearl oyster tissues, which is consistent with patterns reported in other bivalve species and supports the hypothesis that each tissue type represents a unique microenvironment for bacterial colonization. We suggest that, based on the strong effect of tissue-type on the pearl oyster bacterial community, future studies should apply caution when attempting to compare microbial patterns from different locations, and when searching for disease agents. The lack of association with water at each farm also supported the unique nature of the microbial communities in oyster tissues. In contrast to the whole bacterial community, there was no significant difference in the Vibrio community among tissue types nor location. These results suggest that Vibrio species are shared among different pearl oyster tissues. In particular, the similarity between the haemolymph, inside shell and solid tissues, suggests that the haemolymph and inside shell environment is a source of microbial spillover into the oyster tissues, and a potentially useful tool for non-destructive routine disease testing and early warning surveillance. These data provide important foundational information for future studies identifying the factors that drive microbial assembly in a valuable aquaculture species.

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“…Toxin concentrations below 5 µg g −1 do not cause the disease, although that concentration can induce the collapse (atrophy) of the tubular hepatopancreatic epithelium [32]. Atrophied epitheliums have also been observed under experimental conditions in shrimp infected with Vp AHPND doses lower than the infective threshold (<10 4 CFU mL −1 ) [11] in low virulence strains [3,11,36,39] and in surviving shrimp [34]. Under conditions of experimental infection, the atrophied epithelium in surviving shrimp could be the result of a decrease in PirAB production/secretion after the acute stage of AHPND, which reduces the lesions caused by the disease and favors shrimp survival.…”

Section: Histopathology Of Ahpndmentioning

confidence: 99%

New Insights into the Mechanism of Action of PirAB from Vibrio Parahaemolyticus

Soto-Rodríguez

Lozano‐Olvera

Montfort

et al. 2022

Toxins

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PirAB toxins secreted by Vibrio parahaemolyticus (Vp) harbor the pVA1 virulence plasmid, which causes acute hepatopancreatic necrosis disease (AHPND), an emerging disease in Penaeid shrimp that can cause 70–100% mortality and that has resulted in great economic losses since its first appearance. The cytotoxic effect of PirABVp on the epithelial cells of the shrimp hepatopancreas (Hp) has been extensively documented. New insights into the biological role of the PirBVp subunit show that it has lectin-like activity and recognizes mucin-like O-glycosidic structures in the shrimp Hp. The search for toxin receptors can lead to a better understanding of the infection mechanisms of the pathogen and the prevention of the host disease by blocking toxin–receptor interactions using a mimetic antagonist. There is also evidence that Vp AHPND changes the community structure of the microbiota in the surrounding water, resulting in a significant reduction of several bacterial taxa, especially Neptuniibacter spp. Considering these findings, the PirABvp toxin could exhibit a dual role of damaging the shrimp Hp while killing the surrounding bacteria.

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Detection of Vibrio campbellii and V. parahaemolyticus carrying full-length pirAB but only V. campbellii produces Pir toxins

Cited by 14 publications

References 31 publications

Cellular and transcriptomic response to pathogenic and non-pathogenicVibrio parahaemolyticusstrains causing acute hepatopancreatic necrosis disease (AHPND) inLitopenaeus vannamei

Cellular and transcriptomic response to pathogenic and non-pathogenicVibrio parahaemolyticusstrains causing acute hepatopancreatic necrosis disease (AHPND) inLitopenaeus vannamei

Pearl Oyster Bacterial Community Structure Is Governed by Location and Tissue-Type, but Vibrio Species Are Shared Among Oyster Tissues

New Insights into the Mechanism of Action of PirAB from Vibrio Parahaemolyticus

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