Detection of Viroids by RT-PCR

Donovan, N. J.; Chambers, Grant; Cao, Mengmeng

doi:10.1007/978-1-0716-1464-8_13

Cited by 1 publication

(1 citation statement)

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“…Reverse transcription-polymerase chain reaction (RT-PCR) involves extracting RNA from tissue cells, reverse transcribing it into complementary DNA (cDNA) using reverse transcriptase, and finally using cDNA as a template for PCR amplification (Donovan et al, 2022). The RT-PCR method for detecting BVDV was first established by Hertig et al in 1991, based on the p80 and gp53 genes that encode the NS3 and E2 protein, respectively (Hertig et al, 1991).…”

Section: Reverse Transcription-polymerase Chain Reactionmentioning

confidence: 99%

Diagnosis of bovine viral diarrhea virus: an overview of currently available methods

Wang,

Pang

2024

Front. Microbiol.

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Bovine viral diarrhea virus (BVDV) is the causative agent of bovine viral diarrhea (BVD), which results in significant economic losses in the global cattle industry. Fortunately, various diagnostic methods available for BVDV have been established. They include etiological methods, such as virus isolation (VI); serological methods, such as enzyme-linked immunosorbent assay (ELISA), immunofluorescence assay (IFA), and immunohistochemistry (IHC); molecular methods, such as reverse transcription-polymerase chain reaction (RT-PCR), real-time PCR, digital droplet PCR (ddPCR), loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), and CRISPR-Cas system; and biosensors. This review summarizes the current diagnostic methods for BVDV, discussing their advantages and disadvantages, and proposes future perspectives for the diagnosis of BVDV, with the intention of providing valuable guidance for effective diagnosis and control of BVD disease.

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Section: Reverse Transcription-polymerase Chain Reactionmentioning

confidence: 99%