Detection of Virulence Genes in Staphylococcus aureus and Streptococcus agalactiae Isolated from Mastitis in the Middle East

Elbehiry, Ayman; El-Sayed, Mohamed; Marzouk, Eman; Bathich, Yaser

doi:10.9734/bmrj/2015/19237

Cited by 8 publications

(7 citation statements)

References 23 publications

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“…Five isolates (71.4%) out of seven strains of S. agalactiae demonstrated positively to the cfb gene, known as a cell surface protein, that produces a traditional Christie-Atkins-Munch-Peterson (CAMP) phenomenon, which is a typical half-moon forming hemolytic zones on plates of blood agar. This result was stated by El-Behiry et al (2015), who confirmed a broad frequency of cfb gene possessing S. agalactiae.…”

Section: Discussionsupporting

confidence: 67%

Prevalence, Virulence Genes and Antibiogram Susceptibility Pattern of Staphylococcus aureus and Streptococcus agalactiae Isolated from Mastitic Ewes

2022

Int J Vet Sci

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Clinical mastitis is a common disease found in dairy ewes worldwide that results in great economic losses. This study was designed to investigate the prevalence rate of Staphylococcus aureus and Streptococcus agalactiae isolated from clinically mastitic ewes, determine the virulence determinant genes, and identify antimicrobial sensitivity assay of the isolated bacteria. A total of 37 milk samples were collected from 27 ewes diagnosed with clinical mastitis. These revealed 12 isolates of S. aureus (32.4%), followed by 7 isolates of S. agalactiae (18.9%). Real-time polymerase chain reaction was used to identify eight strains of S. aureus as possessing the thermonuclease (nuc) gene, and five isolates of S. agalactiae were positive for the presence of the cell surface protein (cfb) gene. Eight isolates of S. aureus and five isolates of S. agalactiae isolated from dairy ewes with clinical mastitis in El-Sharkia, Egypt, possessed several virulence determinants. The antibiotic sensitivity pattern was performed and a large proportion of isolates of S. aureus were found to be sensitive to Vancomycin, Kanamycin, Ciprofloxacin, and Gentamycin, whereas large proportions were highly resistant to Penicillin G and Tetracycline. S. agalactiae isolates revealed full sensitivity to Vancomycin, Ciprofloxacin, and Oxacillin, but they were highly resistant to Kanamycin and Penicillin G. The virulence gene should be considered when developing prevention and treatment program for clinical mastitis in ewes in regional areas of isolation. It is necessary that the antibiogram findings of drugs with the highest sensitivity be used to replace the currently used antibiotics with those that are more effective.

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Section: Discussionsupporting

confidence: 67%

Prevalence, Virulence Genes and Antibiogram Susceptibility Pattern of Staphylococcus aureus and Streptococcus agalactiae Isolated from Mastitic Ewes

2022

Int J Vet Sci

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“…Eight genes were selected or S. agalactiae , of which four ( rib, cylE, bca, cfb) were determined to be present in the analysed strains. These results were unsurprising because their presence had been previously confirmed in bovine strains in countries such as Brazil, India or Egypt, and the frequency of occurrence of these genes is local in character [ 12 , 19 – 21 ] . The situation differs with respect to the genes scpB, lmb, pavA and bac, whose presence was not detected in the strains we analysed.…”

Section: Discussionmentioning

confidence: 99%

Biofilm production and other virulence factors in Streptococcus spp. isolated from clinical cases of bovine mastitis in Poland

et al. 2017

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BackgroundMastitis is a common disease in dairy cattle throughout the world and causes considerable economic losses each year. An important aetiological agent of this disease is bacteria of the genus Streptococcus; hence, exploring the mechanisms of virulence in these bacteria is an extremely important step for the development of effective prevention programmes. The purpose of our study was to determine the ability to produce biofilm and the occurrence of selected invasiveness factors among bacteria of the genus Streptococcus isolated from cattle with the clinical form of mastitis in northeastern Poland.ResultsMost of the isolates analysed demonstrated an ability to produce biofilm (over 70%). Virulence genes were searched for in the three most common streptococci in our experiment: S. agalactiae, S. uberis and S. dysgalactiae. For S. agalactiae, only four genes were confirmed: rib (33%), cylE (78%), bca (37%), and cfb (100%). The genes pavA, scpB, bac and lmb were not present in any of the tested strains. The dominant serotypes of the species were Ia (n = 8) and II (n = 8), in addition to some strains that were not classified in any of the groups (n = 6). Out of the eight selected genes for S. uberis (sua, pauA/skc, gapC, cfu, lbp, hasA, hasB, hasC), only one was not found (lbp). Finally, two genes were chosen for S. dysgalactiae (eno and napr), and their presence was confirmed in 76% and 86% of the strains, respectively.ConclusionsThe experiment showed that strains of Streptococcus spp. isolated from dairy cattle with clinical cases of mastitis in the northeastern part of Poland possess several invasiveness factors that can substantially affect the course of the disease, and this should be considered when developing targeted prevention programmes.

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“…A perusal search of the literature revealed scarce reports available on the expression of the virulence determinants of S. agalactiae accused of bovine mastitis [ 23 ], however, their existence was numerously investigated. In previous reports, the existence of scpB and lmb genes was reported in S. agalactiae derived from mastitic cattle in the Middle East [ 24 ]. CylE gene was found in bovine S. agalactiae in Poland [ 10 ], while the hyl gene existed in bovine but not in human invasive S. agalactiae isolates in New York [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial and antibiofilm potentials of cinnamon oil and silver nanoparticles against Streptococcus agalactiae isolated from bovine mastitis: new avenues for countering resistance

et al. 2021

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Background Streptococcus agalactiae (S. agalactiae) is a contagious pathogen of bovine mastitis. It has financial implications for the dairy cattle industry in certain areas of the world. Since antimicrobial resistance increases in dairy farms, natural antimicrobials from herbal origins and nanoparticles have been given more attention as an alternative therapy. Hence, this study reported the antimicrobial and antibiofilm potentials of cinnamon oil, silver nanoparticles (AgNPs), and their combination against multidrug-resistant (MDR) S. agalactiae recovered from clinical bovine mastitis in Egypt. Results Our findings revealed that 73% (146/200) of the examined milk samples collected from dairy cows with clinical mastitis were infected with Streptococci species. Of these, 9.59% (14/146) were identified as S. agalactiae and categorized as MDR. S. agalactiae isolates expressed four virulence genes (Hyl, cylE, scpB, and lmb) and demonstrated an ability to produce biofilms. Cinnamon oil showed high antimicrobial (MICs ≤0.063 μg /mL) and antibiofilm (MBIC50 = 4 μg/mL) potentials against planktonic and biofilms of S. agalactiae isolates, respectively. However, AgNPs showed reasonable antimicrobial (MICs ≤16 μg/mL) and relatively low antibiofilm (MBIC50 = 64 μg/mL) activities against screened isolates. Synergistic antimicrobial or additive antibiofilm interactions of cinnamon oil combined with AgNPs were reported for the first time. Scanning electron microscope (SEM) analysis revealed that biofilms of S. agalactiae isolates treated with cinnamon oil were more seriously damaged than observed in AgNPs cinnamon oil combination. Moreover, reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) showed that cinnamon oil exerted a remarkable down-regulation of pili biosynthesis genes (pilA and pilB) and their regulator (rogB) against S. agalactiae biofilms, meanwhile the AgNPs cinnamon oil combination demonstrated a lower efficacy. Conclusions This is an in vitro preliminary approach that documented the antibiofilm potential of cinnamon oil and the inhibitory activity of cinnamon oil and its combination with AgNPs against MDR S. agalactiae recovered from clinical mastitis. Further in vivo studies should be carried out in animal models to provide evidence of concept for implementing these alternative candidates in the treatment of dairy farms infected by streptococcal mastitis in the future.

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Detection of Virulence Genes in Staphylococcus aureus and Streptococcus agalactiae Isolated from Mastitis in the Middle East

Cited by 8 publications

References 23 publications

Prevalence, Virulence Genes and Antibiogram Susceptibility Pattern of Staphylococcus aureus and Streptococcus agalactiae Isolated from Mastitic Ewes

Prevalence, Virulence Genes and Antibiogram Susceptibility Pattern of Staphylococcus aureus and Streptococcus agalactiae Isolated from Mastitic Ewes

Biofilm production and other virulence factors in Streptococcus spp. isolated from clinical cases of bovine mastitis in Poland

Antimicrobial and antibiofilm potentials of cinnamon oil and silver nanoparticles against Streptococcus agalactiae isolated from bovine mastitis: new avenues for countering resistance

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