Detection of White Spot Disease Virus (WSDV) Infection in Shrimp Usingin SituHybridization

Wang, Chiu‐Sen; Tsai, Yu‐Hsia; Chen, S.N.

doi:10.1006/jipa.1998.4771

Cited by 24 publications

(10 citation statements)

References 11 publications

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“…A one‐step immunochromatographic assay for detecting WSSV in shrimp exists (Ko, Ko, Mao & Tseng 2003). Other diagnostic methods for WSSV detection include in situ hybridization (Wang et al . 1998), and the miniarray method (Quere, Commes, Marti, Bonami & Piquemal 2002), which allows one‐step multiple detection of WSSV by hybridization of a PCR product onto a nylon membrane and the visualization of the hybrids by an antibody, increasing pathogen detection considerably.…”

Section: Other Diagnostic Methodsmentioning

confidence: 99%

“…Diseased shrimp exhibit a reduction in feeding and increased lethargy. Infected shrimp swim slowly near the pond surface and eventually sink to the bottom and die (Chang, Lo, Wang & Kou 1996;Lightner 1996;Durand, Lightner, Redman & Bonami 1997;Karunasagar, Otta & Karunasagar 1997;Sudna, Mohan, Shankar & Hagde 1997;Chang, Chen & Wang 1998;Nunan, Poulos & Lightner 1998;Wang,Tsai & Chen 1998;Jory & Dixon 1999;Wang,White, Redman & Lightner 1999a).…”

Section: Pathologymentioning

confidence: 99%

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White Spot Syndrome Virus in cultured shrimp: A review

2007

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Shrimp is one of the main aquaculture species in the world. Different viruses affect them, which causes serious mortality to economically important species, such as Penaeus monodon, Litopenaeus vannamei and L. stylirostris, among others. White spot syndrome virus or WSSV is a highly lethal, stress‐dependent virus, which belongs to the family Nimaviridae, genus Whispovirus. Three WSSV virus isolates were first detected in 1992 in Thailand, Taiwan and China. Later, a fourth isolate of the virus was detected in the Americas in 1999. This virus has a large circular double‐stranded DNA genome with different sizes (292.9–307.2 kb), where the diverse isolates show differences in virulence. This virus infects a wide range of aquatic crustaceans by vertical and horizontal transmission, with different mortality results. The spread of infection between regions may be due to infected shrimp and carriers such as other crustaceans, seabirds, aquatic arthropods or other vectors. The aim of this work is to describe the current knowledge on the status, transmission, pathology, isolation, control and genomic characteristics of WSSV.

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Section: Other Diagnostic Methodsmentioning

confidence: 99%

Section: Pathologymentioning

confidence: 99%

White Spot Syndrome Virus in cultured shrimp: A review

2007

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“…These include PCR (Wang et al . 1997), two‐step PCR (Lo, Leu, Ho, Chen, Peng, Chen, Chou, Yeh, Huang, Chou, Wang & Kou 1996), ISH (Wang, Tsai & Chen 1998), Western blot (Nadala, Tapay, Cao & Loh 1997), dot‐blot nitrocellulose enzyme immunoassay (DB‐NC‐EIA) (Nadala & Loh 2002), competitive ELISA (Hameed, Anilkumar, Raj & Jayaraman 1998), and capture ELISA (Chen et al . 2002).…”

Section: Titration Of White Spot Syndrome Virus (Wssv) In Gill Filtrmentioning

confidence: 99%

Detection and titration of white spot syndrome virus using a Blue‐Cell ELISA

Shih

2002

Journal of Fish Diseases

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“…Many methods have been developed for detection of WSSV, including PCR (Lightener, 1996;Kimura et al, 1996;Lo et al, 1996b;Kim et al, 1998;Kasornchandra et al, 1998;Tapay et al, 1999), in situ PCR (ISPCR) (Jian et al, 2005), in situ hybridization (ISH) (Lightener, 1996;Durand et al, 1996;Chang et al, 1996;Wongteerasupaya et al, 1996;Wang et al, 1998b; Huang * Corresponding author. Tel.…”

Section: Introductionmentioning

confidence: 99%