Detection of white spot syndrome virus (WSSV) of shrimp by means of monoclonal antibodies (MAbs) specific to an envelope protein (28 kDa)

Liu, W; Wang, Y T; Tian, Di; Yin, Zhimin; Kwang, Jimmy

doi:10.3354/dao049011

Cited by 52 publications

(27 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Monoclonal antibodies against Vp28 of WSSV were prepared as described in Liu et al (2002). Briefly, spleen cells obtained from Balb/c mice that were immunized with 3 doses of purified recombinant Vp28 protein were fused with X63Ag8.653 mouse myeloma cells (ATCC) in the presence of PEG 1500.…”

Section: Methodsmentioning

confidence: 99%

White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis

Wang¹,

Liu²,

Seah³

et al. 2002

Dis. Aquat. Org.

Self Cite

View full text Add to dashboard Cite

White spot syndrome virus (WSSV) was specifically detected by PCR in Penaeus merguiensis hemocytes, hemolymph and plasma. This suggested a close association between the shrimp hemolymph and the virus. Three types of hemocyte from shrimp were isolated using flow cytometry. Dynamic changes of the hemocyte subpopulations in P. merguiensis at different times after infection were observed, indicating that the WSSV infection selectively affected specific subpopulations. Immunofluorescence assay (IFA) and a Wright-Giemsa double staining study of hemocyte types further confirmed the cellular localization of the virus in the infected hemocytes. Electron microscopy revealed virus particles in both vacuoles and the nucleus of the semigranular cells (SGC), as well as in the vacuoles of the granular cells (GC). However, no virus could be detected in the hyaline cells (HC). Our results suggest that the virus infects 2 types of shrimp hemocytes -GCs and SGCs. The SGC type contains higher virus loads and exhibits faster infection rates, and is apparently more susceptible to WSSV infection.

show abstract

Section: Methodsmentioning

confidence: 99%

White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis

Wang¹,

Liu²,

Seah³

et al. 2002

Dis. Aquat. Org.

Self Cite

View full text Add to dashboard Cite

show abstract

“…In AmEPV-infected cells, spheroidin alone accounts for up to 30-40% of the total protein of infected insect cells (Winter et al 1995). WSSV also has several highly expressed structural genes/proteins, and, to date, the major structural protein VP28 has been the most commonly used WSSV target for immuno-based detection (Liu et al 2002, Yoganandhan et al 2004. Here, however, we have shown that the transcription expression levels of ICP11 are approximately 3 × greater than those of VP28.…”

Section: Discussionmentioning

confidence: 73%

“…The sensitivity of this kind of test depends on the immuno-detected target protein, and several candidate proteins have already been conside-Identification of icp11, the most highly expressed gene of shrimp white spot syndrome virus (WSSV) red. Liu et al (2002) and Yoganandhan et al (2004) independently developed procedures that targeted the WSSV major envelope protein, VP28, while Okumura et al (2005) developed a reverse passive latex agglutination method to detect WSSV virion particles by using anti-WSSV immunoglobulin G (IgG). All 3 of these studies were based on WSSV structural proteins, which until now were thought to be the most highly expressed proteins of WSSV.…”

Section: Introductionmentioning

confidence: 99%

Identification of icp11, the most highly expressed gene of shrimp white spot syndrome virus (WSSV)

Wang

Kou

et al. 2007

Dis. Aquat. Org.

View full text Add to dashboard Cite

This study investigates white spot syndrome virus (WSSV) gene expression levels in the cells of 2 hosts (Penaeus monodon and Litopenaeus vannamei). Microarray and expressed sequence tag (EST) analysis of the mRNA profiles in WSSV-infected P. monodon cells were used to identify WSSV genes that were very highly expressed. Results showed that the mRNA of the WSSV icp11 gene consistently had the highest copy number of all (3× higher than the major envelope protein, VP28). At the protein level in WSSV-infected L. vannamei, 2-dimensional gel analysis and liquid chromatography-nano-electrospray ionization tandem mass spectrometry (LC-nanoESI-MS/MS) protein identification also showed that this WSSV non-structural protein has the highest expression levels reported to date. ICP11 is capable of self-multimerization, and it becomes located in both the cytoplasm and nucleus of the host cell. These data suggest that ICP11 plays an important, but presently unknown, role during viral infection, and that expression of the WSSV icp11 gene/WSSV ICP11 protein is potentially a good and diagnostically useful indicator of WSSV infection.

show abstract

“…For example, it has been shown with WSSV that ELISA detection is substantially improved by using two complementary MAb that target different epitopes of a single viral coat protein. 21 A similar phage display approach might be applied for improvement of the similar detection strip for white spot syndrome virus (WSSV) of shrimp that uses a gold-labeled MAb specific to the 28 kDa envelope protein VP28 22 and a polyclonal antibody at the trap line. Results from dot-blot ELISA tests with all 13 YBPs (in two replicates) dotted on membranes.…”

Section: Biopanning For Yhv Binding Peptides Biopanning For Yhv Bindimentioning

confidence: 99%

Untitled

Gangnonngiw¹,

Ramasootra²,

Soowannayan³

et al. 2006

ScienceAsia

View full text Add to dashboard Cite

Yellow head virus (YHV) causes a serious disease that can result in high mortality of penaeid shrimp within 2-3 days after the first appearance of gross signs of disease in rearing ponds. Current detection systems for YHV are based on molecular and immunological techniques. Immunological detection methods require continual production of relatively large, complex proteins using living animals or hybridoma cells. An alternative possibility is to use the phage display technique to find short peptides that can bind strongly with YHV particles and replace antibodies in such tests. Once identified, these peptides could also be tested for efficacy in blocking YHV infection. YHV was purified and then immobilized on microtiter plates for 4 rounds of biopanning against a pool of phage displayed peptide variants. From 89 sequentially generated phage pools, 13 variants were selected for strong binding with YHV by ELISA assay. DNA sequencing led to the deduced amino acid sequences LNAKSRN, KSKKSSS, GPQRKRS, KLKRLSS, RTNKKNA, SNISNAS, SNSKKRN, RTKKMRT, NTKRPAR, GPQRKRS, VSNKKRS, RKKSNAS and GPKKNRS. Preliminary tests using two representative phages (SNSKKRN and GPQRKRS) with high YHV binding activity showed that they were unable to block YHV infection in shrimp. However, when immobilized, these clones could bind YHV from probe solutions, indicating that they have potential for use as YHV detection reagents.

show abstract

Detection of white spot syndrome virus (WSSV) of shrimp by means of monoclonal antibodies (MAbs) specific to an envelope protein (28 kDa)

Cited by 52 publications

References 27 publications

White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis

White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis

Identification of icp11, the most highly expressed gene of shrimp white spot syndrome virus (WSSV)

Untitled

Contact Info

Product

Resources

About