Detection of white spot syndrome associated baculovirus in experimentally infected wild shrimp, crab and lobsters by in situ hybridization

Chang, Poh-Shing; Chen, Hsiao-Chao; Wang, Yu-Chi

doi:10.1016/s0044-8486(98)00189-6

Cited by 148 publications

(89 citation statements)

References 6 publications

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“…WSSV has a very wide host range, including P. clarkii (Chang et al, 1998;Baumgartner et al, 2009). Bacterial infections are also very common and can be developed by a variety of Gram positive and Gram negative forms that can inhabit their external exoskeleton, gut and frequently the haemolymph (Thune, 1994).…”

Section: Trophic Ecologymentioning

confidence: 99%

Red swamp crayfish: biology, ecology and invasion - an overview

et al. 2015

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-Alien species have been transported and traded by humans for many centuries. However, with the era of globalization, biological invasions have reached notable magnitudes. Currently, introduction of alien species is one of the major threats to biodiversity and ecosystem functioning. The North American crayfish Procambarus clarkii is one of the most widely introduced freshwater species in the world, especially due to its high economic importance. It is responsible for great modifications in invaded environments causing irreparable ecological and economic damages. Its impressive ability to successfully colonize a wide range of environments is a consequence of its behavioural and biological characteristics that can adapt to features of the invaded location, conferring to this species a notable ecological plasticity. This review summarizes the available information regarding P. clarkii's biology and invasive dynamics around the world in order to contribute to the understanding of the threats posed by its establishment, as well as to support management and impact mitigation efforts.

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Section: Trophic Ecologymentioning

confidence: 99%

Red swamp crayfish: biology, ecology and invasion - an overview

et al. 2015

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“…The WSSV virion consists of at least five major proteins with estimated sizes of 15kDa (VP15), 19 kDa (VP19), 24 kDa (VP24), 26 kDa (VP26) and 28 kDa (VP28) and 13 minor proteins (14-16) with the nucleocapsid (11). The WSSV virions have circular sequence of 292,967 base pairs (bp) (13) but there is variation in size in geographic isolates of WSSV (12,17,18).…”

Section: Indian Joumal Of Clinical Biochemistry 2005mentioning

confidence: 99%

“…Various kinds of rapid diagnostic tools, such as gene probes (19), PCR (18) and immunological methods (20)(21)(22) have been developed to detect the spread of WSSV. The immunological methods are not so accurate and sensitive as compared to PCR but are convenient for the field level detection of WSSV.…”

Section: Indian Joumal Of Clinical Biochemistry 2005mentioning

confidence: 99%

Production of recombinant enveloped structural proteins from the Chinese WSSV isolate

Jha

2005

Indian J Clin Biochem

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The white spot syndrome virus (WSSV) is one of the deadly pathogens of penaeid shrimps and other crustaceans. The WSSV virion consists of an enveloped rod-shaped nucleocapsid enclosing a large circular double stranded DNA genome of 305 Kb with 181 open reading frames. The two major structural genes, VP19 and VP28 were amplified from the genomic DNA of Chinese isolate of WSSV and ctoned in pUCm-T vector and sub cloned in pET-30a (+) vector. The expressions of genes in E. coil (BL21) were confirmed by SDS-PAGE analysis. The clones were sequenced, submitted to the gene bank and the Xiang Shan strain of WSSV were compared with the previous reported sequence of WSSV of various regions which revealed that VP19 and VP28 gene sequences had certain differences from the sequences of similar genes of the isolate already reported. The recombinant proteins expressed, purified and characterized.

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“…White spot syndrome is one of the major infectious viral diseases that cause large scale mortality of commercially cultivated crustacean species which include shrimp, prawn, crab and other arthropods Chang et al 1998;Chen et al 2000). Numerous studies on morphology, histopathology and gene sequence of White Spot Syndrome Virus (WSSV) are reported but the mechanism of WSSV infection is not understood (Jiang et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Susceptibility of testicular cell cultures of crab, Scylla serrata (Forskal) to white spot syndrome virus

Shashikumar

Desai

2012

Cytotechnology

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Testicular cell culture of crab, Scylla serrata (Forskal) was used to study the effects of White spot syndrome virus (WSSV). We are showing the susceptibility of cell culture of crabs to WSSV. The proliferating cell culture of testes were maintained for more than 4 months in a medium prepared from L15 and crab saline supplemented with epidermal growth factor. The cell cultures inoculated with different concentrations of virus showed distinct cytopathic effects such as change in cell appearance, shrinkage and cell lysis. WSSV infection of cultured cells was confirmed by Nested PCR technique. The incorporation of viral DNA in cultured cells was shown by RAPD profile generated using 10-mer primers. The controls that were not exposed to WSSV did not show cytopathic effects. This work shows the usefulness of proliferating testicular cell culture for studying WSSV infection using molecular tools. Thus, this report gains significance as it opens new vistas for diagnostics and drugs for WSSV.

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Detection of white spot syndrome associated baculovirus in experimentally infected wild shrimp, crab and lobsters by in situ hybridization

Cited by 148 publications

References 6 publications

Red swamp crayfish: biology, ecology and invasion - an overview

Red swamp crayfish: biology, ecology and invasion - an overview

Production of recombinant enveloped structural proteins from the Chinese WSSV isolate

Susceptibility of testicular cell cultures of crab, Scylla serrata (Forskal) to white spot syndrome virus

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