Detection of white spot syndrome virus in shrimp by loop-mediated isothermal amplification

Detection methods for milk pathogenic bacteria by loop-mediated isothermal amplification

“…The figures presented in this study suggest that the LAMP assay is 1,000 times more sensitive than PCR for detection of ETEC from raw milk. Most studies using the LAMP reaction for detection have found sensitivity to be equal to or 10 times more than conventional PCR/ RT-PCR (17)(18)(19)(20).…”

PENGARUH PERBEDAAN WAKTU APLIKASI PROBIOTIK TERHADAP KUALITAS AIR DAN SINTASAN PASCA LARVA UDANG WINDU (Penaeus monodon)

“…Kasus penyakit pada budidaya udang beberapa tahun terakhir ini, melanda Indonesi a dan n egara-n egara l ain sep ert i Thailand (Pasharawipas et al, 1998;Ruangpan et al, 1998;Sukhumsirichart et al, 1998), Taiwan (Peng et al, 2001), Filipina (Albaladejo et al, 1998;Loh et al, 1998), India , Australia (Spann et al, 1995), Jepang (Kono et al, 2004), dan Amerika (Dhar et al, 2001), kini sedikit demi sedikit mulai dapat teratasi. Hal ini bukan karena penyakit virus sebagai penyebab utamanya yang dapat diatasi, akan tetapi terutama karena subtitusi dari udang windu ke vaname, meskipun pada kenyataannya bahwa udang vaname juga tidak terlepas dari serangan penyakit terutama WSSV (Rodriguez et al, 2003;SanchezMartinez et al, 2007).…”

A new fluorescent quantitative PCR-based in vitro neutralization assay for white spot syndrome virus

“…et al, 2000), observation of tissues after fixation or negative staining (Wongteerasupaya et al, 1995), immune methods using scFv, monoclonal and polyclonal antibodies against WSSV or their component proteins Nadala and Loh, 2000;Poulos et al, 2001;Liu et al, 2002;Dai et al, 2003), loopmediated isothermal amplification (LAMP) (Kono et al, 2004) and reverse passive latex agglutination (RPLA) (Okumura et al, 2004(Okumura et al, , 2005 assay. However, all of these assays are qualitative.…”