Detection of Xanthomonas axonopodis pv. manihotis, the causal agent of cassava bacterial blight diseases in cassava (Manihot esculenta) in Ghana by polymerase chain reaction

The citrus canker pathogen Xanthomonas axonopodis has caused severe damage to citrus crops worldwide, resulting in significant economic losses for the citrus industry. To address this, a green synthesis method was used to develop silver nanoparticles with the leaf extract of Phyllanthus niruri (GS-AgNP-LEPN). This method replaces the need for toxic reagents, as the LEPN acts as a reducing and capping agent. To further enhance their effectiveness, the GS-AgNP-LEPN were encapsulated in extracellular vesicles (EVs), nanovesicles with a diameter of approximately 30–1000 nm naturally released from different sources, including plant and mammalian cells, and found in the apoplastic fluid (APF) of leaves. When compared to a regular antibiotic (ampicillin), the delivery of APF-EV-GS-AgNP-LEPN and GS-AgNP-LEPN to X. axonopodis pv. was shown to have more significant antimicrobial activity. Our analysis showed the presence of phyllanthin and nirurinetin in the LEPN and found evidence that both could be responsible for antimicrobial activity against X. axonopodis pv. Ferredoxin-NADP+ reductase (FAD-FNR) and the effector protein XopAI play a crucial role in the survival and virulence of X. axonopodis pv. Our molecular docking studies showed that nirurinetin could bind to FAD-FNR and XopAI with high binding energies (−10.32 kcal/mol and −6.13 kcal/mol, respectively) as compared to phyllanthin (−6.42 kcal/mol and −2.93 kcal/mol, respectively), which was also supported by the western blot experiment. We conclude that (a) the hybrid of APF-EV and GS-NP could be an effective treatment for citrus canker, and (b) it works via the nirurinetin-dependent inhibition of FAD-FNR and XopAI in X. axonopodis pv.

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“…X. axonopodis pv. also causes the bacterial blight of cassava [3]. Severe infection by X. axonopodis pv.…”

Section: Introductionmentioning

confidence: 99%

Delivery of Apoplastic Extracellular Vesicles Encapsulating Green-Synthesized Silver Nanoparticles to Treat Citrus Canker

et al. 2023

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“…Phytopathogenic bacteria account for the main losses in crops such as citrus, cassava, banana, rice, wheat, sugarcane, and beans. Their mechanism of action cause symptoms including spots, cankers, rots, and hormonal imbalances that lead to excessive plant growth, dwarfism, branching of roots, and epinastic leaf growth, among others (NADARASAH and STAVRINDES, 2011;TIAN et al, 2016;MARTINS et al, 2018;ABDULAI et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Alternative control of phytopathogenic bacteria with essential oils of Elionurus latiflorus and Cymbopogon flexuosus

Martinazzo

Braga²,

Teodoro³

2022

CeN

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The use of hazardous chemicals has become a common practice to control diseases that affect agricultural production. In this scenario, society is challenged to increase food availability while reducing pesticide use, which causes damage to health and the environment. In this sense, essential oils appear as a promising alternative to reduce the use of pesticides, since they are plant-derived compounds. This study aimed to identify the main chemical components and evaluate the in vitro antimicrobial potential of essential oils of the Brazilian species Elionurus latiflorus and the exotic species Cymbopogon flexuosus against the phytopathogenic bacteria Xanthomonas axonopodis pv. phaseoli, Ralstonia solanacearum, Pectobacterium carotovorum pv. carotovorum and Pseudomonas syringae pv. tomato. The main component identified of the essential oils was citral at the concentrations 65.38% for E. latiflorus and 71.6% for C. citratus. The analysis of the antibacterial activity of the essential oils showed effect against all bacteria analyzed when compared to the antibiotic gentamicin. The antibiotic produced inhibition zone diameters from 11.30 to 20.67 mm, while the essential oils produced the inhibition zones from 51.22 to 90 mm and pure citral around 86 mm. MIC values for essential oils were found between 25 and 200 µL/mL and MBC between 100 and 400 µL/mL. The study showed that the oils have inhibitory effect on the microorganisms tested.

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Genome-wide identification and data mining reveals major-latex protein (MLP) from the PR-10 protein family played defense-related roles against phytopathogenic challenges in cassava (Manihot esculenta Crantz)

Viboonjun,

Longsaward

2024

Genetica

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Detection of Xanthomonas axonopodis pv. manihotis, the causal agent of cassava bacterial blight diseases in cassava (Manihot esculenta) in Ghana by polymerase chain reaction

Cited by 7 publications

References 23 publications

Delivery of Apoplastic Extracellular Vesicles Encapsulating Green-Synthesized Silver Nanoparticles to Treat Citrus Canker

Delivery of Apoplastic Extracellular Vesicles Encapsulating Green-Synthesized Silver Nanoparticles to Treat Citrus Canker

Alternative control of phytopathogenic bacteria with essential oils of Elionurus latiflorus and Cymbopogon flexuosus

Genome-wide identification and data mining reveals major-latex protein (MLP) from the PR-10 protein family played defense-related roles against phytopathogenic challenges in cassava (Manihot esculenta Crantz)

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