Detection of Zika virus RNA in whole blood of imported Zika virus disease cases up to 2 months after symptom onset, Israel, December 2015 to April 2016

Lustig, Yaniv; Mendelson, Ella; Paran, Nir; Melamed, Sharon; Schwartz, Eli

doi:10.2807/1560-7917.es.2016.21.26.30269

Cited by 104 publications

(88 citation statements)

References 17 publications

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“…This is consistent with the observation that the longest period reported between blood donation and symptoms (presymptomatic viremia) was 6 days ( Figure 1A). Because detection methods using whole blood samples have allowed detecting virus RNA up to 68 days after the onset of symptoms, 17 it will be important to determine whether such samples contain infectious virus, and to reassess ZIKV transfusion risk accordingly.…”

mentioning

confidence: 99%

Zika virus in asymptomatic blood donors in Martinique

Gallian

Cabie

Richard

et al. 2017

Blood

113

100

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study of the platelet inhibitor prasugrel in children with sickle cell disease was designed to identify drug doses that inhibit platelet function between 30% and 50%, a level thought to balance safety and efficacy, 24 similar to the level of inhibition observed here, although the dual effects of RN-1 on platelet function and platelet counts could pose an additional risk for bleeding that will require further monitoring.Our results show that administration of RN-1 to normal baboons over a prolonged period increases HbF, F cells, and F retics and is generally well tolerated, supporting further development of LSD1 inhibitors as therapeutic agents for SCD. Because LSD1 also has an important functional role in neural stem cell maintenance and proliferation, effects of LSD1 inhibitors on the brain and nervous system should be carefully evaluated.

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mentioning

confidence: 99%

Zika virus in asymptomatic blood donors in Martinique

Gallian

Cabie

Richard

et al. 2017

Blood

113

100

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“…48 Zika virus RNA appears to persist for a longer interval in whole blood compared with serum or plasma; follow-up testing of 5 individuals yielded detectable RNA in whole blood from 5 to 58 days after symptom onset despite RNA-negative findings in corresponding serum samples. 49 Also, urine samples in this study were RNA positive from 5 to 26 days. It is well documented that Zika virus and RNA persist in urine and semen longer than in plasma.…”

Section: Transfusion Riskmentioning

confidence: 91%

Zika and the Blood Supply: A Work in Progress

Katz¹,

Rossmann²

2016

Archives of Pathology &Amp; Laboratory Medicine

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Zika virus can be transmitted by transfusion, but the harm caused to recipients is not clear in most cases. It is very likely that the virus could also be transmitted by transplanted organs. Sensitivity to the risk from transfusion is elevated by consideration of possible severe neurologic damage in fetuses. Strategies for dealing with transfusion risk vary with the presence of Zika in the region. In nonendemic areas, risks can be reduced by excluding donors who have exposure through travel or sexual contact with someone at risk. In both endemic and nonendemic areas, the risk can be further reduced by nucleic acid testing of donors, or pathogen reduction of platelet and plasma products. The real risk to the population depends on the frequency of infection as well as the efficacy of these interventions. The interventions chosen will depend on the risk assessment for any situation; in the United States at this time, a combination of travel deferrals, testing, and, to a lesser extent, pathogen reduction is being used, but universal testing of US blood donors under investigational use has been mandated by the US Food and Drug Administration, beginning with states most at risk of local transmission. Canada is largely using travel deferrals. A precautionary approach may be taken; however, a formal decision-making framework has been suggested. The situation globally is clearly very fluid, as the epidemic continues to spread and we continue to learn how to best protect recipients of blood and transplants.

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“…This should be confirmed in other studies. Interestingly, Lustig et al recently showed in a small subset of patients that whole blood remained positive for ZIKV RNA longer than serum (24). The few data on PCR testing of saliva suggest that it is more sensitive than blood but less sensitive than urine (8,25).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Altona Diagnostics RealStar Zika Virus Reverse Transcription-PCR Test Kit for Zika Virus PCR Testing

et al. 2017

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With the emerging Zika virus (ZIKV) epidemic, accessible real-time reverse transcription-PCR (rRT-PCR) assays are needed to streamline testing. The commercial Altona Diagnostics RealStar ZIKV rRT-PCR test kit (Altona PCR) has been approved for emergency use authorization by the U.S. FDA. Our aim was to verify the Altona PCR by comparing it to the CDC-designed dual-target ZIKV rRT-PCR reference assay (reference PCR) and describe the demographics of patients tested for ZIKV by rRT-PCR in Ontario, Canada. A large set of clinical specimens was tested for ZIKV by the Altona PCR and the reference PCR. Positive or equivocal specimens underwent PCR and Sanger sequencing targeting the ZIKV NS5 gene. A total of 671 serum specimens were tested by the reference PCR: 58 (8.6%) were positive, 193 (28.8%) were equivocal, and 420 (62.6%) were negative. Ninety percent of the reference PCR-positive patients were tested in the first 5 days after symptom onset. The Altona PCR was performed on 284/671 specimens tested by the reference PCR. The Altona PCR was positive for 53/58 (91%) reference PCR-positive specimens and 16/193 (8%) reference PCR-equivocal specimens; the ZIKV NS5 PCR was positive for all 68 Altona PCR-positive specimens and negative for all 181 Altona PCR-negative specimens that underwent the NS5 PCR. The Altona PCR has very good sensitivity (91%) and specificity (97%) compared to the reference PCR. The Altona PCR can be used for ZIKV diagnostic testing and has less extensive verification requirements than a laboratory-developed test.

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Detection of Zika virus RNA in whole blood of imported Zika virus disease cases up to 2 months after symptom onset, Israel, December 2015 to April 2016

Cited by 104 publications

References 17 publications

Zika virus in asymptomatic blood donors in Martinique

Zika virus in asymptomatic blood donors in Martinique

Zika and the Blood Supply: A Work in Progress

Evaluation of Altona Diagnostics RealStar Zika Virus Reverse Transcription-PCR Test Kit for Zika Virus PCR Testing

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