2020
DOI: 10.1002/rcm.8791
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Detection of α‐defensin in synovial fluids by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry as an innovative and cost‐effective assay for improved definition of periprosthetic joint infections

Abstract: Rationale:Detection of α-defensins in synovial fluid is gaining more and more interest in the field of correct diagnosis of periprosthetic joint infections (PJIs). At present, they can be assessed by a quantitative enzyme-linked immunosorbent assay which is expensive and time-consuming and by a qualitative lateral flow immunoassay which is rapid but quite expensive and whose clinical sensitivity is debated. Thus, developing an alternative rapid, accurate, and low-cost assay for α-defensins is important to make… Show more

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Cited by 3 publications
(3 citation statements)
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“…As recommended by the 2018 ICM, in such cases, further tests can be useful to diagnose or rule out PJI. In this regard, Petrucca et al 30 have recently published an innovative test to identify alpha-defensins using MALDI-TOF MS.…”
Section: Discussionmentioning
confidence: 99%
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“…As recommended by the 2018 ICM, in such cases, further tests can be useful to diagnose or rule out PJI. In this regard, Petrucca et al 30 have recently published an innovative test to identify alpha-defensins using MALDI-TOF MS.…”
Section: Discussionmentioning
confidence: 99%
“…Next, the synovial fluid sample was immediately delivered to the Pathology and Clinical Microbiology Laboratory of our Institute for further evaluation (i.e., synovial WBC, leukocyte esterase strip test, and PMN%). The remaining synovial fluid was assessed for alpha-defensins using MALDI-TOF MS as described by Petrucca et al 30 . Centrifugation at 15,000 rpm for five minutes was performed to remove eventual contamination of tissue debris or blood cells.…”
Section: Synovial Fluid Analysismentioning
confidence: 99%
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