2013
DOI: 10.3390/ijerph10072688
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Detection of β-Lactamase Residues in Milk by Sandwich ELISA

Abstract: β-Lactamase residues in milk represent a public health risk. The cylinder plate detection method, which is based on bacterial growth, is laborious and time consuming. In this study, 15 monoclonal antibodies (mAbs) were selected against Temoneira (TEM) 1 β-lactamase. A sandwich enzyme-linked immunosorbent assay (ELISA) based on an optimum mAb pair was developed and validated for the detection of β-lactamase. The limit of detection and linear dynamic range of the method were 4.17 ng/mL and 5.5–100 ng/mL, respect… Show more

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Cited by 22 publications
(14 citation statements)
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“…The LOD and linear dynamic range of the method were 4.17 ng/mL and 5.5-100 ng/mL, respectively. [18] So this Sandwich ELISA is more sensitive than Wang,W. reported.…”
Section: Discussionmentioning
confidence: 94%
See 1 more Smart Citation
“…The LOD and linear dynamic range of the method were 4.17 ng/mL and 5.5-100 ng/mL, respectively. [18] So this Sandwich ELISA is more sensitive than Wang,W. reported.…”
Section: Discussionmentioning
confidence: 94%
“…[17] A sandwich ELISA based on an monoclonal antibodies (mAb) pair has been described for the detection of Temoneira (TEM) 1 beta-lactamase. [18] In this study, we chose to generate polyclonal and monoclonal antibodies against penicillinase and to produce a sandwich ELISA for the detection of penicillinase in milk. AE260 Electronic analytical balance was from METTLER (Germany).…”
Section: Introductionmentioning
confidence: 99%
“…In the present work, E. coli and K. pneumoniae strains were resistant to cefoxitin and clavulanate, some of them were resistant to cefepim but sensitive to ceftazidime and imipenem (Table 2). This antibiogram might be an indicator for bls-AmpC, in addition, anti CMY polyserum used in bla-NT was raised against bls with molecular weight 38 kb, these bls were obtained from PCR confirmed CMY-bla producing strain [1,15]. Anti-bla CMY used in bla-NT does not crossly react with other lactamases.…”
Section: Discussionmentioning
confidence: 99%
“…Meanwhile, detection by antibody based methods was used with success. Specific antibodies against β-lactamases can neutralize the enzyme reaction and neutralization tests have led to classification of β-lactamases of Gramnegative bacteria into different classes [13][14][15]. ELISA and immunoblotting could screen and quantify AmpC, SHV and TEM bls with high sensitivity and specificity.…”
Section: Introductionmentioning
confidence: 99%
“…Sandwich ELISA was performed as described previously with minor modifications (Wang et al, 2013). Briefly, CD44-exonv3 IgG was first Fig.…”
Section: Sandwich Elisamentioning
confidence: 99%