Detection ofSalmonella species in faeces by latex agglutination in enrichment broth

Benge, G. R.

doi:10.1007/bf01963453

Cited by 8 publications

(3 citation statements)

References 13 publications

(4 reference statements)

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“…Although latex agglutination testing has been successfully used for identification of a variety of clinically important microorganisms [19][20][21][22][23][24][25][26], its use for the detection of non-O157 : H7 strains of Escherichia coli has not been reported. The use of antibodies against cellsurface antigens for the detection or identification of bacteria has been advocated [32], and the approach was applied to detect some bacterial pathogens [33,34].…”

Section: Discussionmentioning

confidence: 99%

“…The sensitivity of the b-glucuronidase test ranged from 84 to 93% [14,15,17]; furthermore, Shigella and Salmonella may cause false-positive results [18]. Latex agglutination tests (LATs) have been successfully used for the rapid identification of a variety of clinically important microorganisms such as Escherichia coli O157 : H7 [19,20], Salmonella [21][22][23], and Staphylococcus aureus [24][25][26]. However, latex agglutination testing has not been evaluated for the detection of other strains of Escherichia coli.…”

Section: Introductionmentioning

confidence: 99%

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Development of a Latex Agglutination Test for Rapid Identification of Escherichia coli

Huang

Chang

2001

EJCMID

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Escherichia coli, one of the most important human pathogens, is usually identified by a battery of biochemical tests that require overnight incubation. For rapid identification of Escherichia coli, a latex agglutination test (LAT) was developed. Rabbits were immunized with cell-surface antigens extracted from Escherichia coli CCRC 15481 with 4 M urea, and the affinity-purified antibodies were used to coat latex particles for the identification of the bacterium. The following gram-negative bacteria were used to evaluate the LAT: Escherichia coli (n = 761), Enterobacteriaceae other than Escherichia coli (n = 632), Aeromonas spp. (n = 21), Pseudomonas spp. (n = 75), Vibrio spp. (n = 18), and other bacteria (n = 64). The LAT had a sensitivity and specificity of 99.2 and 93.3%, respectively. If the LAT was used in conjunction with the tests of indole production or lactose fermentation, the specificity values for the identification of Escherichia coli increased from 93.3 to 98.8 and 98.7%, respectively. If the LAT, indole production, and lactose fermentation were used together for the identification of Escherichia coli, the sensitivity and specificity were 94 and 99.7%, respectively. Lactose fermentation could be detected by observing the colonies grown on selective media (e.g. MacConkey agar), and indole production could be analyzed simply by the spot indole test. Strains producing negative reactions (i.e. not identified as Escherichia coli) should be processed by the conventional procedures for identification. The present protocol integrating the LAT, indole production, and lactose fermentation for the identification of Escherichia coli offers considerable savings of time, manpower, and cost.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of a Latex Agglutination Test for Rapid Identification of Escherichia coli

Huang

Chang

2001

EJCMID

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“…Most of the procedures reported have been enzyme immunoassays that relied on polyclonal or monoclonal antibodies (mcabs) to detect lipopolysaccharide for specificity (for example: Rigby 1984; Chaicumpa et al 1988;Beumer et al 1991; Luk and Lindberg 1991;Choi et al 1992). Other techniques such as latex agglutination (Lim 1990;Benge 1989) have also been developed. The sensitivities of these immunological tests is in the range of nanogram per ml of lipopolysaccharide (LPS) .…”

Section: Introductionmentioning

confidence: 99%

COMPARISON of ANALYTICAL SENSITIVITIES of THREE ENZYME IMMUNOASSAY PROCEDURES FOR the DETECTION of SALMONELLA LIPOPOLYSACCHARIDE

Nielsen

Tsang

1992

Rapid Methods Automation Mic

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7he analytical sensitivities of three diflerent enzyme linked immunoassays (ELISA), two competitive and a capture fomt.were assessed. The assay systems employed monoclonal antibodies to Salmonella lipopolysaccharide (LPS) outer core epitopes to detect crude LPS antigens from Salmonella typhimurium. The most sensitive ELISA was the capture procedure, being capable of detection 1.3 nglml of LPS. This technique, however also gave the greatest between-test variation and as a result, the lowest amount that could be detected with a 95% conjidence limit was actually 12.8 ng/ml and it took the longest time to perform (3 h, 30 min). A competitive ELISA using limiting monoclonal antibody to compete between solid phase antigen and soluble antigen in the sample, ranked second in sensitivity, and can detect 2.8 and 3.8 ng/ml of LPS when tested with two d$ferent monoclonal antibodies. However, because of the slight between test variation, the actual sensitivities that could be detected with a 95% confidence limit were 3.1 and 4.6 ng/ml, respectively. This test takes approximately 1 h and 30 min to perform.The classical type of competitive assay, employing a labelled antigen, was the least sensitive being capable of detecting 5.8 ng/ml if the LPS was conjugated with horseradish peroxidase and 16.0 ng/ml if alkaline phosphatase was used as a label. To account for the between-test variation, the sensitivities with a 95 % confidence limit were 8.6 and 18.7 ng/ml for the respective assays, which take 2 h and 15 min to perform.These sensitivities compare favorably with those published for similar assays, but all of the procedures were judged insuflciently sensitive for direct use on food samples to be tested for the presence of Salmonella species. However, the msays would be quite suitable for demonstration of Salmonella sp. afrer an enrichment procedure.

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Monoclonal antibodies that identify gram-negative bacteria using the magnetic immunoluminescence assay

Torensma

Visser

Aarsman

et al. 1992

Journal of Microbiological Methods

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Detection ofSalmonella species in faeces by latex agglutination in enrichment broth

Cited by 8 publications

References 13 publications

Development of a Latex Agglutination Test for Rapid Identification of Escherichia coli

Development of a Latex Agglutination Test for Rapid Identification of Escherichia coli

COMPARISON of ANALYTICAL SENSITIVITIES of THREE ENZYME IMMUNOASSAY PROCEDURES FOR the DETECTION of SALMONELLA LIPOPOLYSACCHARIDE

Monoclonal antibodies that identify gram-negative bacteria using the magnetic immunoluminescence assay

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