DETECTION, PURIFICATION, AND IDENTIFICATION OF SIDEROPHORES PRODUCED BY Pseudomonas fluorescens ISOLATES USING SDS-PAGE AND HPLC.

El-Sheikh,; El-Kazzaz, S.A.; Hafez, Elsayed E.; Madkour, Samia; El-Gayyar, S.M.

doi:10.21608/jppp.2011.86516

Cited by 2 publications

(3 citation statements)

References 15 publications

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“…This might be attributed to the negative transcriptional control by fur protein where ferrous iron (Fe +2 ) functions as a co-repressor for the producing organism. These lower levels of iron were also confirmed for siderophore production from P. aeruginosa FP6 (5 µM) [19], P. aeruginosa strain ryn32 (25 µM) [15], Pseudomonas strain GRP3A [14], and three P. fluorescens isolates (0 µM) [20].…”

Section: Discussionsupporting

confidence: 52%

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Anticandidal Activity of a Siderophore from Marine Endophyte Pseudomonas aeruginosa Mgrv7

Kotb,

Al-Abdalall,

Ababutain

et al. 2024

Antibiotics

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An endophytic symbiont P. aeruginosa-producing anticandidal siderophore was recovered from mangrove leaves for the first time. Production was optimal in a succinate medium supplemented with 0.4% citric acid and 15 µM iron at pH 7 and 35 °C after 60 h of fermentation. UV spectra of the acidic preparation after purification with Amberlite XAD-4 resin gave a peak at 400 nm, while the neutralized form gave a peak at 360 nm. A prominent peak with RP-HPLC was obtained at RT 18.95 min, confirming its homogeneity. It was pH stable at 5.0–9.5 and thermally stable at elevated temperatures, which encourages the possibility of its application in extreme environments. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) against Candida spp. Were in the range of 128 µg/mL and lower. It enhanced the intracellular iron accumulation with 3.2–4.2-fold (as judged by atomic absorption spectrometry) with a subsequent increase in the intracellular antioxidative enzymes SOD and CAT. Furthermore, the malondialdehyde (MDA) concentration due to cellular lipid peroxidation increased to 3.8-fold and 7.3-fold in C. albicans and C. tropicalis, respectively. The scanning electron microscope (SEM) confirmed cellular damage in the form of roughness, malformation, and production of defensive exopolysaccharides and/or proteins after exposure to siderophore. In conclusion, this anticandidal siderophore may be a promising biocontrol, nonpolluting agent against waterborne pathogens and pathogens of the skin. It indirectly kills Candida spp. by ferroptosis and mediation of hyperaccumulation of iron rather than directly attacking the cell targets, which triggers the activation of antioxidative enzymes.

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Section: Discussionsupporting

confidence: 52%

“…YPD broth was selected for the growth of tested species. It consisted of (g/L) peptone (20), dextrose sugar (20 g/L), and yeast extract (10) with a pH adjusted to 7.0. Two loopfuls from the active growth of Candida spp.…”

Section: Sem Studymentioning

confidence: 99%

Anticandidal Activity of a Siderophore from Marine Endophyte Pseudomonas aeruginosa Mgrv7

Kotb,

Al-Abdalall,

Ababutain

et al. 2024

Antibiotics

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“…The three components function as the ABC protein, membrane fusion protein, and outer membrane protein, respectively [ 2 ]. T1SS, such as TliDEF, bypass the periplasm, allowing the secretion of proteins with diverse sizes [ 3 ]. In addition to that, its simple growth conditions and ability to reach acceptable cell density without optimization make P. fluorescens an interesting biological vehicle for recombinant proteins [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Utilizing the ABC Transporter for Growth Factor Production by fleQ Deletion Mutant of Pseudomonas fluorescens

et al. 2021

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Pseudomonas fluorescens, a gram-negative bacterium, has been proven to be a capable protein manufacturing factory (PMF). Utilizing its ATP-binding cassette (ABC) transporter, a type I secretion system, P. fluorescens has successfully produced recombinant proteins. However, besides the target proteins, P. fluorescens also secretes unnecessary background proteins that complicate protein purification and other downstream processes. One of the background proteins produced in large amounts is FliC, a flagellin protein. In this study, the master regulator of flagella gene expression, fleQ, was deleted from P. fluorescens Δtp, a lipase and protease double-deletion mutant, via targeted gene knockout. FleQ directs flagella synthesis, so the new strain, P. fluorescens ΔfleQ, does not produce flagella-related proteins. This not only simplifies purification but also makes P. fluorescens ΔfleQ an eco-friendly expression host because it will not survive outside a controlled environment. Six recombinant growth factors, namely, insulin-like growth factors I and II, beta-nerve growth factor, fibroblast growth factor 1, transforming growth factor beta, and tumor necrosis factor beta, prepared using our supercharging method, were successfully secreted by P. fluorescens ΔfleQ. Our findings demonstrate the potential of P. fluorescens ΔfleQ, combined with our supercharging process, as a PMF.

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DETECTION, PURIFICATION, AND IDENTIFICATION OF SIDEROPHORES PRODUCED BY Pseudomonas fluorescens ISOLATES USING SDS-PAGE AND HPLC.

Cited by 2 publications

References 15 publications

Anticandidal Activity of a Siderophore from Marine Endophyte Pseudomonas aeruginosa Mgrv7

Anticandidal Activity of a Siderophore from Marine Endophyte Pseudomonas aeruginosa Mgrv7

Utilizing the ABC Transporter for Growth Factor Production by fleQ Deletion Mutant of Pseudomonas fluorescens

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