Detection the specific marker of CD3 molecules of human peripheral blood T lymphocytes using SNOM and quantum dots

Zhong, Liu; Liao, Wentao; Xiao-pin, Wang; Cai, Jun

doi:10.1016/j.colsurfa.2007.04.173

Cited by 16 publications

(8 citation statements)

References 13 publications

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“…These results were consistent with what were seen by AFM topographic and force-binding analyses. We have shown that nanoclusters or nanodomains seen in NSOM imaging of CD3 (Zhong et al, 2008) and Vg2Vd2 TCR (Chen et al, 2008) usually reflect TCR signaling and activation events. Occurrence of CD69 nanoclusters after PHA activation may promote development of CD4 þ effector function of cytokine production or others.…”

Section: Resultsmentioning

confidence: 97%

“…In particular, functionalization of the AFM tip with ligands has allowed mapping the distribution of complementary receptors on model or cellular surfaces (Dupres et al, 2005;Hinterdorfer and Dufrene, 2006;Dupres et al, 2007). On the other hand, we and other have shown that the near-field scanning optical microscopy (NSOM)-and quantum dot (QD)-based nanoscale fluorescence imaging is a useful nanotechnology tool for studying nano-spatial aspects of immune molecules on the membrane of T cells (de Lange et al, 2001;Rasmussen and Deckert, 2005;Novotny and Stranick, 2006;Chen et al, 2008;Zhong et al, 2008). Here we employed the combined AFM topographic and atomic force-based binding and mapping (www.interscience.wiley.com) DOI:10.1002/jmr.976 nanotechnology to visualize early expression of CD69 on defined membrane ultrastructures of PHA-activated CD4 þ T cells.…”

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confidence: 97%

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AFM‐ and NSOM‐based force spectroscopy and distribution analysis of CD69 molecules on human CD4⁺ T cell membrane

Chen

Wang

et al. 2009

J of Molecular Recognition

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Although CD69 is well known as an early T cell-activation marker, the possibility that CD69 are distributed as nano-structures on membrane for immune regulation during T cell activation has not been tested. In this study, nanoscale features of CD69 expression on activated T cells were determined using the atomic force microscopy (AFM) topographic and force-binding nanotechnology as well as near-field scanning optical microscopy (NSOM)-/fluorescence quantum dot (QD)-based nanosacle imaging. Unstimulated CD4(+) T cells showed neglectable numbers of membrane CD69 spots binding to the CD69 Ab-functinalized AFM tip, and no detectable QD-bound CD69 as examined by NSOM/QD-based imaging. In contrast, Phytohemagglutinin (PHA)-activated CD4(+) T cells expressed CD69, and displayed many force-binding spots binding to the CD69 Ab-functionalized AFM tip on about 45% of cell membrane, with mean binding-rupture forces 276 +/- 71 pN. Most CD69 molecules appeared to be expressed as 100-200 nm nanoclusters on the membrane of PHA-activated CD4(+) T cells. Meanwhile, NSOM/QD-based nanoscale imaging showed that CD69 were non-uniformly distributed as 80-200 nm nanoclusters on cell-membrane of PHA-activated CD4(+) T cells. This study represents the first demonstration of the nano-biology of CD69 expression during T cell activation.

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Section: Resultsmentioning

confidence: 97%

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confidence: 97%

AFM‐ and NSOM‐based force spectroscopy and distribution analysis of CD69 molecules on human CD4⁺ T cell membrane

Chen

Wang

et al. 2009

J of Molecular Recognition

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“…Blood cells have previously been investigated with SNOM . The fluorescence of hemoglobin is undetectable due to its fast non‐radiative decay .…”

Section: Resultsmentioning

confidence: 99%

High‐resolution Raman imaging reveals spatial location of heme oxidation sites in single red blood cells of dried smears

Marzec

Ryguła

Wood

et al. 2014

J Raman Spectroscopy

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Raman confocal microscopy with 488 nm excitation wavelength supported with atomic force microscopy (AFM), scanning near‐field optical microscopy (SNOM) and UV–Vis spectrometry was used to investigate air‐dried erythrocytes (red blood cells, RBCs) in whole human blood smears. The central internal part of the cell was dominated by the laser‐induced O2 dissociated oxyHb form as evidenced by the Fe2+ marker band appearing at 1356 cm−1. The existence of a thin outer layer of hemoglobin in the periphery of RBCs was assigned to hemichrome. Evidence for hemichrome includes the oxidation state marker band appearing at 1376 cm−1, the absence of FeO2 band at 570 cm−1 and a UV–Vis spectrum consistent with hemichrome. This is the first time that distributions of Fe2+/Fe3+ hemes inside the single RBC have been reported. The outer layer formation of hemichrome was additionally studied when RBCs were in contact with leucocytes and carotenoids of blood plasma. Copyright © 2014 John Wiley & Sons, Ltd.

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“…SNOM has been used to investigate the localisation of molecules within cell membranes of prostate cancer cells 11 . Further research demonstrated SNOM can accurately define both the cell surface and internal structures in both healthy and anomalous sperm, including the acrosome, nucleus and the organisation of mitochondria 12 , and has demonstrated potential for single molecule imaging 13 . The application of SNOM to oesophageal cancer tissue studies provided evidence of its potentiality for cancer diagnosis 8 .…”

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confidence: 99%

Imaging cervical cytology with scanning near-field optical microscopy (SNOM) coupled with an IR-FEL

Halliwell

Morais

Lima

et al. 2016

Sci Rep

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Cervical cancer remains a major cause of morbidity and mortality among women, especially in the developing world. Increased synthesis of proteins, lipids and nucleic acids is a pre-condition for the rapid proliferation of cancer cells. We show that scanning near-field optical microscopy, in combination with an infrared free electron laser (SNOM-IR-FEL), is able to distinguish between normal and squamous lowgrade and high-grade dyskaryosis, and between normal and mixed squamous/glandular pre-invasive and adenocarcinoma cervical lesions, at designated wavelengths associated with DNA, Amide I/II and lipids. These findings evidence the promise of the SNOM-IR-FEL technique in obtaining chemical information relevant to the detection of cervical cell abnormalities and cancer diagnosis at spatial resolutions below the diffraction limit (≥0.2 μm). We compare these results with analyses following attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy; although this latter approach has been demonstrated to detect underlying cervical atypia missed by conventional cytology, it is limited by a spatial resolution of ~3 μm to 30 μm due to the optical diffraction limit.Cervical cancer is associated with the persistent infection of high-risk types of human papillomavirus (HPV), together with other socioeconomic co-factors 1 . Screening involves cytological and histological classification of cervical cells. In the UK, cytological examination of cervical squamous cells is classified as normal, borderline or mild dyskaryosis, moderate or severe dyskaryosis and invasive cervical cancer (ICC). Histology is defined as normal, cervical intra-epithelial neoplasia (CIN): CIN1, CIN2, CIN3, or invasive cervical cancer. For atypical cells found in the glandular cells of the cervix, the pre-invasive lesion of adenocarcinoma is defined by changes termed cervical glandular intraepithelial neoplasia (CGIN), and sub-classified as low-grade cervical glandular intra-epithelial neoplasia (LGCGIN) and high-grade cervical glandular intra-epithelial neoplasia (HGCGIN). Squamous and glandular lesions may co-exist together and are defined by the level of CIN together with either LGCGIN or HGCGIN. Conventional screening is flawed as it is dependent on the subjective visual inspection of cytology; this often results in mis-diagnoses when grading samples 2 .

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Detection the specific marker of CD3 molecules of human peripheral blood T lymphocytes using SNOM and quantum dots

Cited by 16 publications

References 13 publications

AFM‐ and NSOM‐based force spectroscopy and distribution analysis of CD69 molecules on human CD4⁺ T cell membrane

AFM‐ and NSOM‐based force spectroscopy and distribution analysis of CD69 molecules on human CD4⁺ T cell membrane

High‐resolution Raman imaging reveals spatial location of heme oxidation sites in single red blood cells of dried smears

Imaging cervical cytology with scanning near-field optical microscopy (SNOM) coupled with an IR-FEL

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Detection the specific marker of CD3 molecules of human peripheral blood T lymphocytes using SNOM and quantum dots

Cited by 16 publications

References 13 publications

AFM‐ and NSOM‐based force spectroscopy and distribution analysis of CD69 molecules on human CD4+ T cell membrane

AFM‐ and NSOM‐based force spectroscopy and distribution analysis of CD69 molecules on human CD4+ T cell membrane

High‐resolution Raman imaging reveals spatial location of heme oxidation sites in single red blood cells of dried smears

Imaging cervical cytology with scanning near-field optical microscopy (SNOM) coupled with an IR-FEL

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AFM‐ and NSOM‐based force spectroscopy and distribution analysis of CD69 molecules on human CD4⁺ T cell membrane

AFM‐ and NSOM‐based force spectroscopy and distribution analysis of CD69 molecules on human CD4⁺ T cell membrane