Deteksi Molekuler Entamoeba suis pada Babi di Rumah Potong Hewan Pegirian Surabaya

Beka, Fransiska Cicilia; Lastuti‬, ‪Nunuk Dyah Retno; Suwanti, Lucia Tri; Koesdarto, Setiawan; Mufasirin, Mufasirin; Kusumawati, Dyah

doi:10.19087/jveteriner.2021.22.3.383

Cited by 3 publications

(5 citation statements)

References 13 publications

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“…The oocysts were round with a diameter of 3.67 μm (Figure 1). This is consistent with the findings of Mufa et al (2020) who reported that the size of the oocysts of Cryptosporidium spp. ranged from two to six μm, were round, and had four sporozoites inside.…”

Section: Resultssupporting

confidence: 93%

“…Cryptosporidiosis is a waterborne zoonosis caused by microbial contamination or hazardous substances that are transmitted between living organisms through water (Mufa et al, 2020). According to the World Health Organization (WHO), waterborne diseases are responsible for 4.1% of total deaths worldwide, affecting approximately 1.8 million people annually (WHO, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Detection of Cryptosporidium spp. in Wild Rats (Rattus spp.) in Surabaya, East Java

Pratama,

Suprihati,

Suwanti

et al. 2024

MKH

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Cryptosporidiosis is a waterborne zoonosis caused by Cryptosporidium, which is a parasite that causes infection of the small intestine and leads to acute diarrhea in both humans and animals. Wild rats (Rattus spp.) are known to carry many zoonotic pathogens including Cryptosporidium. Given their close proximity to humans in urban environments, the likelihood of disease transmission is high. This study aims to detect the Cryptosporidium spp. infection in wild rats in Surabaya City, East Java Province. Intestinal fecal samples were collected using necropsy. The flotation test and Ziehl-Neelsen stain were used to observe the presence of Cryptosporidium under the microscope. The results showed that 69 samples were positive for Cryptosporidium spp. in the oocyst stage, which appeared dark pink with a clear cavity and the size of the oocysts ranged from 2-6 μm. The high incidence of cryptosporidiosis in wild rats is a potential health threat to both animals and humans.

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Section: Resultssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Detection of Cryptosporidium spp. in Wild Rats (Rattus spp.) in Surabaya, East Java

Pratama,

Suprihati,

Suwanti

et al. 2024

MKH

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“…Perlu dilakukan pemeriksaan secara molekuler dengan metode PCR untuk mengetahui tingkat spesies dari parasit ini. PCR dapat mengidentifikasi mikroorganisme secara detail hingga tingkat spesies bahkan strain yang tidak dapat dilakukan menggunakan sistem konvensional dengan pemeriksaan mikroskopis (Mufa et al, 2020).…”

Section: Hasil Dan Pembahasanunclassified

Parasit Gastrointestinal pada Kadal Naga Berjanggut (Pogona vitticeps) di Cijeruk, Bogor

Noor Rifai,

Nugraha,

Handharyani

et al. 2023

Jurnal Sain Veteriner

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Bearded dragons are reptiles that are quite popular as pets. There are many factors of disease that can infect bearded dragons. The environment and feed affect the level of disease threat, such as parasitic infection in the gastrointestinal tract. Data information related to parasitic gastrointestinal infections in bearded dragons in Indonesia is still limited, so supporting examinations are necessary to help diagnose. A total of 38 fecal samples were collected from the bearded dragon in Cijeruk Bogor. Samples were examined by simple flotation method, Ziehl Neelsen staining, and counting the number of parasitic eggs. All samples were positive for gastrointestinal parasite infections such as Choleoeimeria sp., Isospora amphiboluri, Cryptosporidium spp., ascarid egg, and oxyurid egg. The average number of Choleoeimeria sp. and Isospora amphiboluri oocysts were 8730 and 51.281 oocysts per gram of feces. The average number of helminth eggs per gram was 290 and 2325 for ascarid and oxyurid, respectively. Most of the infected bearded dragons do not show significant clinical symptoms. The number of oocysts and worm eggs per gram of feces showed mild and high results.

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“…Metode identifikasi Entamoeba yang umum dilakukan adalah pemeriksaan feses secara mikros kopis berdasarkan karakter morfologinya. Akan tetapi, metode tersebut memiliki kelemahan tidak dapat meng identifikasi spesies Entamoeba dikarenakan ukuran yang sangat kecil dan hampir seragam antar spesies (Beka et al, 2021). Entamoeba sp.…”

Section: Hasil Dan Pembahasanunclassified

“…Pada penelitian ini diduga adanya salah satu Oosista Cryptosporidium parvum yang berbentuk bulat dengan ukuran 2,19 2,23 μm pada metode modified Ziehl-Nelseen sedangkan pada metode floatasi ditemukan salah satu oosista Cryptosporidium parvum berukuran 2,87 2,25 μm. Menurut Mufa et al (2020), ukuran oosista Cryptosporidium sp. berukuran 2-6 μm berbentuk bulat atau oval.…”

Section: Hasil Dan Pembahasanunclassified

Identifikasi Protozoa Gastrointestinal pada Orangutan Sumatera (Pongo abelii) dan Orangutan Kalimantan (Pongo pygmaeus) di Taman Satwa Sumatera Utara

Anggraini,

Hanafiah,

Rahmi

et al. 2023

Jurnal Sain Veteriner

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Infeksi protozoa merupakan salah satu penyakit parasitik yang umumnya bersifat kronis serta menyebabkan penurunan berat badan. Penelitian ini bertujuan untuk mengetahui protozoa gastrointestinal yang menginfeksi orangutan sumatera (Pongo abelii) dan orangutan kalimantan (Pongo pygmaeus) yang berada di Taman Satwa Sumatera Utara serta protozoa dari genus apa saja yang menginfeksi orangutan tersebut. Sampel pada penelitian ini menggunakan sampel feses yang didapat dari 1 individu orangutan sumatera dan 2 individu orangutan kalimantan. Sampel diambil tiga kali dalam kurun waktu yang berbeda dengan interval 3 minggu. Sampel feses diambil pada pagi hari kemudian ditambahkan dengan formalin 10% dengan perbandingan 1:1. Pemeriksaan sampel dengan menggunakan metode floatasi, metode modified Ziehl-Neelsen, dan metode sedimentasi formol-eter. Hasil dari penelitian ini menunjukkan bahwa 2 dari 3 orangutan di Taman Satwa Sumatera Utara positif terinfeksi protozoa gastrointestinal. Orangutan sumatera bernama Stevee tidak terifeksi protozoa gastrointestinal dan orangutan kalimantan bernama Simba dan Hamidah positif terinfeksi genus protozoa gastrointestinal yaitu, Entamoeba sp., Balantidium sp. dan Cryptosporidium parvum.

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Deteksi Molekuler Entamoeba suis pada Babi di Rumah Potong Hewan Pegirian Surabaya

Cited by 3 publications

References 13 publications

Detection of Cryptosporidium spp. in Wild Rats (Rattus spp.) in Surabaya, East Java

Detection of Cryptosporidium spp. in Wild Rats (Rattus spp.) in Surabaya, East Java

Parasit Gastrointestinal pada Kadal Naga Berjanggut (Pogona vitticeps) di Cijeruk, Bogor

Identifikasi Protozoa Gastrointestinal pada Orangutan Sumatera (Pongo abelii) dan Orangutan Kalimantan (Pongo pygmaeus) di Taman Satwa Sumatera Utara

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