DETEKSI Piper yellow mottle virus (PYMoV) PENYEBAB PENYAKIT KERDIL PADA TANAMAN LADA SECARA Polymerase Chain Reaction (PCR)

Miftakhurohmah, Nfn; Mariana, Maya; Wahyuno, Dono

doi:10.21082/bullittro.v27n1.2016.77-83

Cited by 10 publications

(10 citation statements)

References 2 publications

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“…Therefore, further investigation regarding the use of chitosan in reducing the CMV infection on black pepper is required, particularly pre-application of chitosan prior to infection and increasing the concentration of chitosan application. Some reports also suggest that stunting disease in black pepper was known has two viruses infection, those viruses were PYMoV and CMV (de Silva et al, 2002;Miftakhurohmah et al, 2016). The use of infected cuttings from the field in this study is likely carry multiple infections.…”

Section: 34mentioning

confidence: 63%

“…Multiple infections of these viruses would generally cause more severe symptoms and inhibit plant growth. In addition, the recognition of visual symptoms in the field was very difficult due to their infection occurred at the same time (Miftakhurohmah et al, 2016). CMV is a single-stranded RNA virus with a broad host range and causes various symptoms.…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Chitosan Application against Plant Growth and Intensity of Stunting Disease on Black Pepper (Piper nigrum L.) Seedlings

Uge

Sulandari

Hartono

et al. 2018

JPTI

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Black pepper (Piper nigrum L.) is an important estate crops in Indonesia. Some pathogens that have been known to infect black pepper plants include fungi, nematodes and viruses. The stunting disease on black pepper plants was caused by Cucumber mosaic virus (CMV). Molecular detection using RT-PCR method showed that the samples were positively infected by CMV which were amplified by specific primers CMV 111 with bands of 111 bp in size. This virus can be carried by vegetative propagation material of plants. Many control strategies against this virus have been investigated, especially inducing plant resistance with chitosan. Chitosan is a natural biopolymer that play an important role in reducing disease incidence and severity and stimulate plant growth. The aim of this study was to figure out the inhibiting ability of chitosan solution against infection of stunting virus on black pepper seedlings through spraying applications. Chitosan treatments were prepared in concentrations of 0.5%, 0.75%, and 1%. The result showed that application of chitosan at all concentrations affected the decrease of disease incidence and intensity and improved plant growth with insignificant different amongst all treatments but significantly different with control. The highest decrease in incidence was found at 0.75% of chitosan concentration (26.37), while the highest decrease of intensity was expressed at 1% of chitosan (37.62). Application of chitosan also significantly affected to all parameters of plant growth either plant height or leaf diameter. Application of 1% of chitosan increased the percentage of plant growth rather than other treatments, with the increase of plant height 58.12 % and leaf diameter 54.74 %. IntisariLada (Piper nigrum L.) merupakan salah satu tanaman perkebunan penting di Indonesia. Beberapa patogen telah diketahui menginfeksi tanaman lada di antaranya jamur, nematoda, dan virus. Penyakit kerdil pada tanaman lada disebabkan oleh Cucumber mosaic virus (CMV). Deteksi molekuler menggunakan metode RT-PCR menunjukkan bahwa sampel positif terinfeksi CMV yang diamplifikasi menggunakan primer spesifik CMV 111 dengan ukuran pita band target 111 bp. Virus ini dapat terbawa bahan perbanyakan tanaman secara vegetatif. Banyak strategi pengendalian virus yang telah diuji, diantaranya induksi ketahanan tanaman dengan kitosan. Kitosan adalah biopolimer alami yang berperan dalam menurunkan insidensi dan intensitas penyakit dan menstimulasi pertumbuhan tanaman. Tujuan dari penelitian ini adalah untuk mengetahui kemampuan penghambatan dari larutan kitosan terhadap infeksi dari virus kerdil pada bibit lada dengan aplikasi penyemprotan. Konsentrasi kitosan yang digunakan adalah 0,5%; 0,75%; dan 1%. Hasil penelitian menunjukan bahwa apliksi kitosan pada semua konsentrasi berpengaruh dalam menurunkan insidensi dan intensitas penyakit dan meningkatkan pertumbuhan tanaman dengan tidak berbeda nyata di antara perlakuan tetapi berbeda nyata dengan kontrol. Penurunan nilai insidensi tertinggi yakni pada aplikasi kitosan 0,75% (26,37), sedangkan penurunan nilai intensitas tertinggi yakni pada aplikasi kitosan 1% (37,62). Aplikasi kitosan juga berpengaruh signifikan terhadap semua parameter pertumbuhan tanaman baik tinggi tanaman maupun diameter daun. Pada aplikasi kitosan 1% meningkatkan persentase tinggi tanaman lebih baik dibandingkan dengan perlakuan lainnya,yakni tinggi tanaman sebesar 58,12 % dan diameter daun sebesar 54,74 %.

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Section: 34mentioning

confidence: 63%

Section: Introductionmentioning

confidence: 99%

The Effect of Chitosan Application against Plant Growth and Intensity of Stunting Disease on Black Pepper (Piper nigrum L.) Seedlings

Uge

Sulandari

Hartono

et al. 2018

JPTI

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“…Al., 2018). The correct identification of which virus infects the plant is necessary to support the development of phytosanitary defense strategies for black pepper crop, since more than one virus can infect the same plant (Miftakhurohmah et al, 2016). Pepper culture faces problems due to the occurrence of rampant viral diseases, and biotechnological tools can be used to solve this problem by identifying virus-free material via PCR, propagate on a large scale in a short time and space, in addition to ensuring phytosanitary quality throughout the process via tissue culture (Bhat et al 2009;.…”

Section: Resultsmentioning

confidence: 99%

IDENTIFICATION AND MICROPROPAGATION OF VIRUS-FREE BLACK PEPPER GENOTYPES (Piper nigrum L.)

et al. 2020

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Virus infections are a major constraint for black pepper cultivation (Piper nigrum L.), a vegetatively propagated crop; therefore, the in vitro selection of virus- free plants is an alternative to reestablish phytosanitary quality of pepper culture. The aim of this study was to select and micropropagate PYMoV virus-free pepper plants. ‘Apra’, ‘Bragantina’, ‘Cingapura’, ‘Clonada’, hybrids and ‘Kottanadan’ genotypes were used in the indexing process. From these, ‘Cingapura’, ‘Clonada’, hybrids and ‘Kottanadan’ were virus free, and only ‘Cingapura’ and ‘Clonada’ were selected for micropropagation. In the multiplication phase, ‘Cingapura’ genotype showed higher multiplication rate in relation to ‘Clonada’ in in vitro rooting and in acclimatization, response was similar in both genotypes. It was possible to identify virus-free genotypes of commercial interest, selecting them for micropropagation, and in this stage, influence of genotype is of great importance.

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“…Hal ini diduga karena titer virus pada sampel tersebut rendah. Deteksi lebih lanjut secara PCR menunjukkan PYMoV terdeteksi pada bibit lada di Sukabumi (Miftakhurohmah et al 2016), dan di Purbalingga (data tidak ditampilkan). Hasil yang sama juga pernah dilaporkan pada deteksi PYMoV yang menginfeksi lada di Sri Lanka, yaitu menunjukkan hasil negatif secara ELISA, tetapi terdeteksi secara PCR (de Silva et al 2002).…”

Section: Deteksi Virus Pymov Dan CMVunclassified

DETEKSI CMV DAN PYMoV PADA BENIH LADA (Piper nigrum) DENGAN TEKNIK ELISA

Mariana

Miftakhurohmah

2017

bul. littro

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ABSTRAKSalah satu kendala penting pada budidaya tanaman lada yaitu penyakit kerdil yang disebabkan oleh Piper yellow mottle virus (PYMoV) dan Cucumber mosaic virus (CMV). Tindakan pencegahan penyebaran penyakit dapat dilakukan salah satunya dengan penggunaan benih sehat bebas dari infeksi PYMoV dan CMV. Tujuan penelitian adalah mendeteksi keberadaan PYMoV dan CMV pada benih lada siap tanam secara serologi menggunakan teknik ELISA. Benih lada yang dideteksi berumur 5 bulan, berasal dari penangkar benih di Sukabumi (Jawa Barat) dan Purbalingga (Jawa Tengah). Varietas lada yang diambil di Sukabumi adalah Natar 1, Petaling, dan Lampung Daun Kecil., masingmasing sebanyak 10 sampel, sedangkan di Purbalingga hanya ada Natar 1, diambil 30 sampel. Di setiap lokasi pembibitan juga dilakukan pengamatan terhadap gejala infeksi virus yang ditemukan. Deteksi virus dilakukan secara Double Antibody Sandwich (DAS)-ELISA menggunakan antiserum Banana streak virus (BSV) untuk PYMoV dan antiserum CMV untuk deteksi CMV. Hasil ELISA dibaca nilai absorbannya dengan elisa reader pada panjang gelombang 405 nm. Sampel dinilai positif, jika nilai absorbansinya 1,5 kali lebih besar daripada kontrol negatif. Gejala infeksi virus pada benih bervariasi, yaitu klorotik, belang, dan belang disertai perubahan bentuk daun. Hasil pengujian ELISA menunjukkan bahwa 66% benih lada dari Sukabumi dan 46% dari Purbalingga terinfeksi virus CMV. Namun, tidak ada satu pun yang menunjukkan reaksi positif terhadap antiserum PYMoV. Hal ini diduga karena konsentrasi PYMoV terlalu rendah sehingga tidak terdeteksi secara ELISA. Untuk mencegah penyebaran virus, deteksi virus pada benih lada penting dilakukan sebelum digunakan sebagai bahan tanaman. Kata kunci: Piper nigrum, ELISA, virus ABSTRACT One important problem on the cultivation of black pepper plants is dwarf disease caused by Piper yellow mottle virus (PYMoV) and Cucumber mosaic virus (CMV). The purpose of this research was to detect the presence of CMV and

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DETEKSI Piper yellow mottle virus (PYMoV) PENYEBAB PENYAKIT KERDIL PADA TANAMAN LADA SECARA Polymerase Chain Reaction (PCR)

Cited by 10 publications

References 2 publications

The Effect of Chitosan Application against Plant Growth and Intensity of Stunting Disease on Black Pepper (Piper nigrum L.) Seedlings

The Effect of Chitosan Application against Plant Growth and Intensity of Stunting Disease on Black Pepper (Piper nigrum L.) Seedlings

IDENTIFICATION AND MICROPROPAGATION OF VIRUS-FREE BLACK PEPPER GENOTYPES (Piper nigrum L.)

DETEKSI CMV DAN PYMoV PADA BENIH LADA (Piper nigrum) DENGAN TEKNIK ELISA

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