Detergent-Compatible Bacterial Amylases

Abstract

Proteases, lipases, amylases, and cellulases are enzymes used in detergent formulation to improve the detergency. The amylases are specifically supplemented to the detergent to digest starchy stains. Most of the solid and liquid detergents that are currently manufactured contain alkaline enzymes. The advantages of using alkaline enzymes in the detergent formulation are that they aid in removing tough stains and the process is environmentally friendly since they reduce the use of toxic detergent ingredients. Amylases active at low temperature are preferred as the energy consumption gets reduced, and the whole process becomes cost-effective. Most microbial alkaline amylases are used as detergent ingredients. Various reviews report on the production, purification, characterization, and application of amylases in different industry sectors, but there is no specific review on bacterial or fungal alkaline amylases or detergent-compatible amylases. In this mini-review, an overview on the production and property studies of the detergent bacterial amylases is given, and the stability and compatibility of the alkaline bacterial amylases in the presence of the detergents and the detergent components are highlighted.

Molecular cloning and characterization of a halotolerant α-amylase from marine metagenomic library derived from Arabian Sea sediments

“…The kinetic values, K m and V max , were calculated from the Lineweaver-Burk plot to be 2.7 mg/mL and 454 U/mL, respectively. The K m of the most detergent compatible amylases was identified to be in the range 0.1-5.0 mg/mL using soluble starch as substrate (Niyonzima and More 2014). A K m value of 3.28 mg/mL has been reported for a a-amylase from metagenomic using amylopectin as substrate (Vester et al 2015).…”

Effect of Water pH on Domestic Machine Washing Performance of Delicate Textiles

“…Amylase and protease enzymes are common detergent enzymes in the laundry detergent market. These two enzymes generally have an optimum pH level at the alkaline region [43,44]. Consequently, these enzymes cannot work efficiently to remove protein-based stains at acidic pH values.…”

Response surface methodology based optimization and scale-up production of amylase from a novel bacterial strain, Bacillus aryabhattai KIIT BE-1

“…The protease of the facultatively anaerobic, reportedly the biggest (length 4 μm, dia. 1.5 μm) known bacterium, Bacillus megaterium [ 1 ] found in diverse habitats, is widely used in detergent industry [ 2 ]. Another soil Bacillus spp., B. licheniformis , produces alkali-tolerant protease suitable for the laundry industry [ 3 ] and lipase [ 4 ] for food industry.…”