Determinación histológica de regenerantes de Euchile mariae (Ames) Withner, (Orchidaceae), obtenidos a partir de protocormos cultivados in vitro

Abstract

From the germination of seeds of Euchile mariae in modified MS medium, the formation of pro- tocorms was achieved. Once these reached an average size from 2 to 5 mm long and the formation of his first leaf primordium, they were used like explants to induce a morphogenetic response. Through in vitro culture of top and bottom protocorms sections, were obtained differentiated structures from asexual origin. Their morphology was similar to protocorms obtained from the germination of seeds, in this way we call them protocorm like bodies (PLB’s). Through of the histological analysis of these structures it was possible to reveal that these PLB’s turned out to be somatic embryos. The histochemical tests demonstrate the pres- ence of cellular contents like: proteins, lipids and starch; both in the cells of the embryos as well as in the cells of initial tissues.

Effects of Climate Change on the Distribution of Prosthechea mariae (Orchidaceae) and within Protected Areas in Mexico

“…The models identified a potential expansion of the species’ suitable distribution area in the eastern part of the country, particularly in the Huasteca region, encompassing Tamaulipas, San Luis Potosí, Hidalgo, Puebla, and Veracruz [ 48 , 49 , 50 ]. This finding aligns with historical collection records and information reported by Soto-Arenas [ 48 ] regarding the species’ distribution.…”

“…P. mariae is recognized to inhabit mid-elevation forests (~800–1350 masl), predominantly among oak and liquidambar species. Its presence has been documented in the states of Tamaulipas, San Luis Potosí, Hidalgo, Puebla, and Veracruz [ 48 , 49 , 50 ]. Its growth occurs between May and November, thriving during the rainy season; flowering takes place in the early summer months, between May and June, with seed dispersal between March and April, during the dry months [ 83 ].…”