Determinación mediante prueba cometa del daño genético causado por uso de ivermectina en vacas cebú (Bos taurus indicus)

Montes-Vergara, Donicer; Ossa, Jaime De La; Cordero, Alexander Pérez

doi:10.21897/rmvz.1034

Cited by 4 publications

(3 citation statements)

References 11 publications

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“…There is also very little information on the use of the comet test in cattle. It has been used in genetic toxicology monitoring, e.g., of the effect of ivermectin, copper, or lead on the DNA structure [ 97 , 98 , 99 , 100 ], and to identify damage to genetic material induced by oxidative stress in cow embryos [ 101 ] and in cows with Bovine papillomavirus [ 102 ]. Tharwat et al [ 103 ] also used SCGE to investigate apoptosis in the peripheral blood cells of dairy cows three weeks before expected parturition, during the week of parturition, and after three weeks.…”

Section: Discussionmentioning

confidence: 99%

Effect of Selected Micro- and Macroelements and Vitamins on the Genome Stability of Bovine Embryo Transfer Recipients following In Vitro Fertilization

Wójcik

Kępka

Skup

2023

Animals

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Genome instability can lead to a wide variety of diseases. Many endogenous and exogenous factors influence the level of damage to genetic material. Genome integrity depends on factors such as the fidelity of DNA replication, normal DNA organization in the chromosomes, and repair mechanisms. Genome stability influences fertility, embryonic development, and the maintenance of pregnancy. In the case of in vitro fertilization, it can be an important factor determining the success of the procedure. The aim of the study was to assess the stability of the genomes of recipient cows following in vitro fertilization using cytogenetic tests and to analyze the effects of selected vitamins and micro- and macroelements on genome integrity. Genome stability was analyzed using the sister chromatid exchange, fragile site, and comet assays. The material for analysis was peripheral blood from 20 Holstein-Friesian heifers that were embryo transfer recipients. The effect of selected micro- and macroelements and vitamins on the genome stability of the cows was analyzed. Folic acid was shown to significantly influence the level of damage identified using the SCE, FS, and SCGE assays, while iron affected SCE and SCGE results, and zinc affected FS.

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Section: Discussionmentioning

confidence: 99%

Effect of Selected Micro- and Macroelements and Vitamins on the Genome Stability of Bovine Embryo Transfer Recipients following In Vitro Fertilization

Wójcik

Kępka

Skup

2023

Animals

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“…El clima de la zona de estudio, es característico de las zonas de bosque seco tropical, persisten relictos de vegetación secundaria; se dan rastrojos y extensas áreas de pastizales. La temperatura promedio anual es de 27.2°C, con precipitaciones fluctuantes ente 900 y 1.275 mm anuales y la humedad relativa del 80% (12,13). Las unidades de producción seleccionadas por municipio, realizan usualmente manejo tradicional para la cría de estirpe criolla (Gallus gallus domesticus -Phasianidae).…”

Section: Materials Y Métodosunclassified

Caracterización morfológica de la gallina criolla de traspatio de la subregión Sabana departamento de Sucre (Colombia)

Montes-Vergara

Ossa

2019

Rev MVZ Córdoba

Self Cite

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Objetivo. Caracterizar morfométrica y faneróptica de la gallina Criolla de traspatio de la subregión Sabana, departamento de Sucre (Colombia). Materiales y métodos. Se evaluaron 520 animales adultos criollos (350 gallinas y 170 gallos), en el periodo 2017-2018, ubicados en 10 localidades de la subregión Sabanas del departamento de Sucre Colombia. Se recolectó la información en un formato de encuesta integrado por variables cuantitativas y cualitativas. Los datos obtenidos fueron sometidos a análisis descriptivos univariado para muestras independiente. Además, se empleó la técnica de análisis de varianza. Las comparaciones entre machos y hembras se realizaron mediante una prueba de t. Los análisis fueron realizados usando paquete estadístico R. Resultados. Los descriptores analizados mostraron superioridad de los gallos sobre las gallinas (p<0.05). El análisis de varianza en la población de hembras con respecto al peso, determinó que existen diferencias significativas (p<0.05). Las características morfológicas y fanerópticas estudiadas, describen un ave de metatarso amarillo, con color de plumaje que combinan tonalidades marrones, negras, gris y blanco, la morfología y distribución de las plumas en la mayoría de las aves es de característica normal (lisa típica), con distribución uniforme a lo largo del cuerpo, aunque se pueden encontrar ejemplares con patrones de plumaje diferente. Conclusiones. Se pudo observar una amplia variación entre animales procedentes de las 10 localidades estudiadas. Se evidencia una diferenciación tipológica con relación al peso y al tamaño del tarso, en los animales evaluados, lo que puede ser utilizado como criterio de selección.

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“…Teratogenic effects were also associated with the use of ivermectin in mice, rats, and rabbits [13]. In addition, a recent study demonstrated potential damage to DNA from the use of ivermectin in zebu cows [14]. Increase in the levels of diptera resistance to insecticides, along with environment and public health concerns associated with the continued use of some neurotoxic insecticides, has stimulated a search for alternative treatments and controls [9,15,16].…”

Section: Introductionmentioning

confidence: 99%

Development of Baccharis dracunculifolia (Asteraceae) Essential Oil Nanoemulsion and Its Biological Activity on Pre-pupae of Cochliomyia hominivorax (Diptera: Calliphoridae)

Seugling¹,

Kuhnen²,

Barros³

et al. 2019

JPP

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Myiasis is considered a serious medical condition in animals and humans that occurs predominantly in tropical countries. Cochliomyia hominivorax is one of the main fly species that causes myiasis and the actual geographic distribution includes the Caribbean and all of South America, except Chile. The use of synthetic insecticides and endectocides in livestock for the myiasis treatment has resulted in insect resistance, while also causing residuals in the environment and contamination of animal food products. Biologically active compounds are considered an alternative for the sustainable management of insects, as they are biodegradable and less damaging to the environment. This study developed a nanoemulsion containing the essential oil of Baccharis dracunculifolia and evaluated its in vitro effects on C. hominivorax larvae. High pressure homogenization was used to develop the nanoemulsion. Larvicidal tests were carried out with the tested products using 15 larvae/repetition, for a total of 150 larvae per treatment. Five different concentrations of the nanoemulsion were tested shortly after preparation and after 120 days of storage. For the fresh formulation, at concentrations of 5, 7.5, 10, 13.5, and 15% (w/v) we found mortality of 28, 48, 70, 84, and 97%, respectively, while for the stored formulation, we found mortality of 17, 36, 51, 81, and 92%. The larvicidal effect was similar, except for the concentrations of 5 and 10% (w/v) (p < 0.05). Our results show that the natural based B. dracunculifolia nanoemulsion can be considered a promising alternative for the treatment and control of myiasis caused by C. hominivorax.

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Determinación mediante prueba cometa del daño genético causado por uso de ivermectina en vacas cebú (Bos taurus indicus)

Cited by 4 publications

References 11 publications

Effect of Selected Micro- and Macroelements and Vitamins on the Genome Stability of Bovine Embryo Transfer Recipients following In Vitro Fertilization

Effect of Selected Micro- and Macroelements and Vitamins on the Genome Stability of Bovine Embryo Transfer Recipients following In Vitro Fertilization

Caracterización morfológica de la gallina criolla de traspatio de la subregión Sabana departamento de Sucre (Colombia)

Development of Baccharis dracunculifolia (Asteraceae) Essential Oil Nanoemulsion and Its Biological Activity on Pre-pupae of Cochliomyia hominivorax (Diptera: Calliphoridae)

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