Determinantes da situação do egresso de gestão ambiental da Universidade de Brasília – UnB

Almeida, Alexandre Nascimento de

doi:10.14244/198271991728

Cited by 1 publication

(2 citation statements)

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“…guineensis cell suspensions in response to different concentrations of auxin [71–73]. The GBSS1 gene is involved in amylose synthesis [74] and histological studies performed by Kanchanapoom and Domyoas [75] and Almeida et al [35] showed the accumulation of starch granules during the induction of SE oil palm. These results may explain the increased transcription of GBSS1 at 30, 90 and 150 doi in the R genotype when compared to the NR genotype.…”

Section: Resultsmentioning

confidence: 99%

“…Proteins potentially involved in the acquisition of embryogenic competence identified in proteomic studies performed by our research group [35] were selected and the corresponding gene was searched for primer design, including PGM , ACT1 , ANN1 , CAT2 , OASA , ENO1 , MDAR5 , PFP-BETA , RUBA , PDIL1-4 , PRB1 , PRB1-3 , EF1 and HSP81-1 (Table 1). Some genes were also selected from previous published studies, such as LEA , FIE2 and GBSS1 [36], BGLUC and SERk1 [23] as well as the reference genes PD00380 [37] and ACT2 [38] (Table 1).…”

Section: Methodsmentioning

confidence: 99%

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Genotype-dependent changes of gene expression during somatic embryogenesis in oil palm hybrids (Elaeis oleifera x E. guineensis)

et al. 2018

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To understand the molecular processes triggered during the different steps of somatic embryogenesis (SE) in oil palm, the expression of 19 genes associated to SE identified in proteomic and transcriptomic studies was investigated by qRT-PCR. To evaluate the differential expression of these genes, two interspecific hybrid genotypes (Elaeis oleifera x Elaeis guineensis) contrasting for the acquisition of embryogenic competence were used. Aclorophyllated leaves of both hybrids, one responsive (B351733) and the other non-responsive (B352933) to SE were submitted to callus induction and collected at different time points: 0 (before induction), 14, 30, 90 and 150 days of callus induction (doi). The results obtained showed that all evaluated genes were downregulated at 14 doi in the responsive genotype when compared to the non-responsive. It was also possible to observe that most of the genes changed their expression behavior at 30 doi and were upregulated thereafter until 150 doi, with the exception of the pathogenesis-related PRB1-3-like (PRB1-3) gene, which did not show differential expression at 30 doi and was downregulated at 90 and 150 doi when compared to the non-responsive hybrid. These results indicate that 30 doi is a turning point in gene expression, probably associated to embryogenic competence acquisition. We also show that the expression behavior of the responsive genotype is more stable than that of the non-responsive when the different induction time points are compared to 0 doi (before induction). Moreover, the results obtained in this study corroborate our hypothesis that the regulation of genes involved in the control of oxidative stress and energy metabolism are crucial for the acquisition of embryogenic competence in oil palm.

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