Determinants and kinetics of cytokine expression patterns in lungs of vaccinated mice challenged with respiratory syncytial virus

Tang, Yi‐Wei; Neuzil, Kathleen M.; Fischer, Julie E.; Robinson, F W; Parker, Robert A.; Graham, Barney S.

doi:10.1016/s0264-410x(96)00214-9

Cited by 21 publications

(14 citation statements)

References 28 publications

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“…It has been demonstrated that immunization with FI-RSV (8,12,38,45,58) or with RSV G (1, 16, 25, 40, 53, 54) predisposes for severe RSV-induced disease typified by enhanced illness, pulmonary eosinophilia, and type 2 cytokine production in the BALB/c mouse model. While the end points may be similar, it is apparent that the cytokine requirements for these two immunogens are distinct.…”

Section: Discussionmentioning

confidence: 99%

“…Vaccine-enhanced disease was observed in 80% of FI-RSV vaccinees in the 1960s trial (27,30) and occurs in many animal models (4,8,25,26,43,45,58) and thus does not appear to be dependent upon a specific genetic background. In contrast, some elements of the immune responses associated with RSV G-induced illness appear to be genetically restricted, such as pulmonary eosinophilia, which is absent or dramatically reduced in mouse strains other than BALB/c and other H-2 d -restricted mouse strains (20,24,55).…”

Section: Figmentioning

confidence: 99%

“…This vaccine-enhanced disease is typified by pulmonary eosinophilia and the production of type 2 cytokines, especially interleukin-4 (IL-4) and IL-5. Interference with the function of these cytokines decreases disease severity (8,24,26,(56)(57)(58), underscoring their importance in mediating FI-RSV vaccine-enhanced immunopathogenesis.…”

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confidence: 99%

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Respiratory Syncytial Virus (RSV) G Glycoprotein Is Not Necessary for Vaccine-Enhanced Disease Induced by Immunization with Formalin-Inactivated RSV

Johnson

Teng

Collins

et al. 2004

J Virol

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Following respiratory syncytial virus (RSV) challenge, mice immunized with RSV G or with formalininactivated RSV (FI-RSV) exhibit severe disease associated with type 2 cytokine production and pulmonary eosinophilia. This has led to the proposal that the presence of RSV G is the factor in FI-RSV that induces disease-enhancing T-cell responses. Therefore, we evaluated the role of RSV G and its immunodominant region in the induction of aberrant immune responses during FI-RSV immunization. BALB/c mice were immunized with FI preparations of wild-type (wt) RSV or recombinant RSV (rRSV) containing deletions of (i) the entire G gene, (ii) the region of the G gene encoding amino acids 187 to 197 of the immunodominant region, or (iii) the entire SH gene. After challenge, illness, RSV titers, cytokine levels, and pulmonary eosinophilia were measured. Peak RSV titers postchallenge were significantly greater in mice immunized with FI preparations of the deletion viruses than in those immunized with FI-rRSV wt, suggesting that the absence of G or SH in FI-RSV reduced its protective efficacy. Deletion of G or its epitope did not reduce illness, cytokine production, or eosinophilia relative to that in mice immunized with FI-rRSV wt. While cytokine levels and eosinophilia were similar, illness was reduced in mice immunized with SH-deleted FI-RSV. These data suggest that G-specific immune responses may be important for vaccine-induced protection and are not solely the basis for FI-RSV vaccine-enhanced illness. These data suggest that the method of RSV antigen delivery, rather than the protein composition, influences the phenotype of the induced immune responses and that RSV G should not necessarily be excluded from potential vaccine strategies.

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Section: Discussionmentioning

confidence: 99%

Section: Figmentioning

confidence: 99%

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Respiratory Syncytial Virus (RSV) G Glycoprotein Is Not Necessary for Vaccine-Enhanced Disease Induced by Immunization with Formalin-Inactivated RSV

Johnson

Teng

Collins

et al. 2004

J Virol

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“…It has been clearly demonstrated that both immunization with FI-RSV (9,13,35,41,50) and immunization with RSV G (4,17,24,38,45,46) predispose for severe disease typified by severe illness, pulmonary eosinophilia, and type 2 cytokine production. However, although the endpoints may be similar, it is apparent that the cytokine requirements for these two immunogens are distinct.…”

Section: Discussionmentioning

confidence: 99%

“…This vaccine-enhanced disease is typified by pulmonary eosinophilia and the production of type 2 cytokines, especially interleukin-4 (IL-4), IL-5, and pulmonary eosinophilia. Interference with the function of these cytokines decreases disease severity (9,23,25,(48)(49)(50), underscoring the importance of cytokines in FI-RSV vaccine-enhanced immunopathogenesis.…”

mentioning

confidence: 99%

Vβ14⁺T Cells Mediate the Vaccine-Enhanced Disease Induced by Immunization with Respiratory Syncytial Virus (RSV) G Glycoprotein but Not with Formalin-Inactivated RSV

Johnson

Varga

Braciale

et al. 2004

J Virol

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Th1- And Th2-Type Cytokine Expression by Activated Tlymphocytes From the Lung and Spleen During the Inflammatory Response to Respiratory Syncytial Virus

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Determinants and kinetics of cytokine expression patterns in lungs of vaccinated mice challenged with respiratory syncytial virus

Cited by 21 publications

References 28 publications

Respiratory Syncytial Virus (RSV) G Glycoprotein Is Not Necessary for Vaccine-Enhanced Disease Induced by Immunization with Formalin-Inactivated RSV

Respiratory Syncytial Virus (RSV) G Glycoprotein Is Not Necessary for Vaccine-Enhanced Disease Induced by Immunization with Formalin-Inactivated RSV

Vβ14⁺T Cells Mediate the Vaccine-Enhanced Disease Induced by Immunization with Respiratory Syncytial Virus (RSV) G Glycoprotein but Not with Formalin-Inactivated RSV

Th1- And Th2-Type Cytokine Expression by Activated Tlymphocytes From the Lung and Spleen During the Inflammatory Response to Respiratory Syncytial Virus

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Determinants and kinetics of cytokine expression patterns in lungs of vaccinated mice challenged with respiratory syncytial virus

Cited by 21 publications

References 28 publications

Respiratory Syncytial Virus (RSV) G Glycoprotein Is Not Necessary for Vaccine-Enhanced Disease Induced by Immunization with Formalin-Inactivated RSV

Respiratory Syncytial Virus (RSV) G Glycoprotein Is Not Necessary for Vaccine-Enhanced Disease Induced by Immunization with Formalin-Inactivated RSV

Vβ14+T Cells Mediate the Vaccine-Enhanced Disease Induced by Immunization with Respiratory Syncytial Virus (RSV) G Glycoprotein but Not with Formalin-Inactivated RSV

Th1- And Th2-Type Cytokine Expression by Activated Tlymphocytes From the Lung and Spleen During the Inflammatory Response to Respiratory Syncytial Virus

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Vβ14⁺T Cells Mediate the Vaccine-Enhanced Disease Induced by Immunization with Respiratory Syncytial Virus (RSV) G Glycoprotein but Not with Formalin-Inactivated RSV