Post-harvest loss posed a challenge for Nigeria's agricultural sector. Post-harvest losses in the production of Nsukka-yellow pepper may result from inadequate handling, poor harvesting methods, pests, and microbial infestation, among other things. As a result, the study determined the post-harvest losses among Nsukka-yellow pepper farmers in Enugu State, Nigeria. Multi-stage sampling technique was used to sample eighty (80) yellow pepper producers from five (5) communities in Nsukka Local Government Area. Primary data were collected using a structured questionnaire. In addition, data were analyzed using descriptive statistics and OLS multiple regression. The OLS multiple regression was used to determine the factors affecting yellow pepper post-harvest loss which was measured by farmers' profitability. The results show that most of the farmers were female, with a mean age of 48 years, and an average of 8 years farming experience. It is significant to highlight that the majority of farmers had no access to credit. The findings also indicated that farmers' profitability was positively influenced by marital status, education, and household size. Furthermore, Nsukka-yellow pepper farmers had post-harvest problems due to insufficient funds, poor storage facilities, and high cost of labour. Hence, Nsukka-yellow pepper farmers are encouraged to access credit facilities in order to increase their capacity to purchase adequate production and harvesting inputs such as storage facilities and similar equipment and technology.