BackgroundThe global COVID-19 pandemic poses substantial health challenges. Vaccination is key in preventing COVID-19, particularly among healthcare workers (HCWs), essential in curbing the pandemic. Despite global data on HCWs’ vaccine uptake, information about vaccination among HCWs in the Somali Region, Ethiopia, is scarce.ObjectiveThis study aimed to assess COVID-19 uptake and determinant factors for HCWs in the Somali Region, Eastern Ethiopia, 2021.MethodsA facility-based cross-sectional study was conducted from 20 October 2021 to 30 October 2021, involving 440 systematically selected participants. Data were collected through self-administered questionnaires, cleaned, coded and entered into Epi Info software (V.3.5.1; CDC). Subsequently, the data were exported to Stata software (V14.1) for analysis. Descriptive statistics were used to report frequency, percentage, mean and SD. Multiple logistic regression analysis was performed to predict the relationship between independent and dependent variables. In the final model, statistical significance was determined with a 95% CI and a p<0.05.ResultA total of 427 HCWs were involved in this study. About 192 (45%) of the participants had a history of test for COVID-19 and 64 (15%) were tested positive for COVID-19 infection. Three hundred and three (76%) at 95% CI (70.3% to 80.6%) of HCWs got at least one dose of the COVID-19 vaccine. Other professions such as paramedics, environmental health and pharmacist were less likely to get COVID-19 vaccine than nurses (adjusted OR, AOR 0.2; 95% CI (0.06 to 0.53)). Participants who were concerned about the availability of the COVID-19 vaccine were less likely to receive it than individuals who were not concerned (AOR 0.01; 95% CI (0.002 to 0.052)).ConclusionThe vaccination uptake among HCWs in the region was relatively low, with the type of profession and concerns about vaccine availability being significant factors affecting the uptake.