Determinants of Dental Pulp Stem Cell Heterogeneity

Nel, Sulette; Durandt, Chrisna; Murdoch, Candice; Pepper, M.

doi:10.1016/j.joen.2022.06.013

Cited by 9 publications

(5 citation statements)

References 84 publications

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“…The identification of the specific substances responsible for maintaining pulp homeostasis is still uncertain. Furthermore, although DPCs have been selected as the primary focus of this study, it is essential to acknowledge their inherent heterogeneity, as different cell types possess distinct markers and functions [62]. Therefore, future research endeavors may identify subpopulations of DPCs that exhibit responsiveness to sensory nerves, aiming to enhance our comprehension of the underlying biological mechanisms within the dental pulp.…”

Section: Discussionmentioning

confidence: 99%

The Role of Sensory Nerves in Dental Pulp Homeostasis: Histological Changes and Cellular Consequences after Sensory Denervation

Wang,

Liu,

Zhou

et al. 2024

IJMS

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Homeostatic maintenance is essential for pulp function. Disrupting pulp homeostasis may lead to pulp degeneration, such as fibrosis and calcifications. Sensory nerves constitute a crucial component of the dental pulp. However, the precise involvement of sensory nerves in pulp homeostasis remains uncertain. In this study, we observed the short-term and long-term histological changes in the dental pulp after inferior alveolar nerve transection. Additionally, we cultured primary dental pulp cells (DPCs) from the innervated and denervated groups and compared indicators of cellular senescence and cellular function. The results revealed that pulp fibrosis occurred at 2 w after the operation. Furthermore, the pulp area, as well as the height and width of the pulp cavity, showed accelerated reductions after sensory denervation. Notably, the pulp area at 16 w after the operation was comparable to that of 56 w old rats. Sensory denervation induced excessive extracellular matrix (ECM) deposition and increased predisposition to mineralization. Furthermore, sensory denervation promoted the senescence of DPCs. Denervated DPCs exhibited decelerated cell proliferation, arrest in the G2/M phase of the cell cycle, imbalance in the synthesis and degradation of ECM, and enhanced mineralization. These findings indicate that sensory nerves play an essential role in pulp homeostasis maintenance and dental pulp cell fate decisions, which may provide novel insights into the prevention of pulp degeneration.

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Section: Discussionmentioning

confidence: 99%

The Role of Sensory Nerves in Dental Pulp Homeostasis: Histological Changes and Cellular Consequences after Sensory Denervation

Wang,

Liu,

Zhou

et al. 2024

IJMS

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“…The autologous dental pulp we used originated from asymptomatic, non-functional third molars, and doctors usually recommend prophylactic extraction of such wisdom teeth based on clinical experience [46]. Thus, wisdom teeth as biological waste can be effectively used [47]. The pulp obtained from wisdom teeth contains many natural scaffolds of DPSC, a pluripotent stem cell with mesenchymal stem cell (MSC)-like characteristics.…”

Section: Discussionmentioning

confidence: 99%

Pulp regeneration by transplantation of dental pulp with the synergy of concentrate growth factor: An in vitro and in vivo study

Xie¹,

Feng²,

Hu³

et al. 2023

Preprint

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Objectives This study aimed to investigate the mechanism of successful autologous pulp transplantation through semi-in situ pulp regeneration in animal experiments and three case reports of autologous pulp transplantation protocols using concentrated growth factor (CGF)-enriched pulp. Material and methods Wisdom tooth pulp was removed, placed in the anterior molar canal, and implanted in the subcranial space of the cranial apex of SD rats to establish an animal model of hemi-in situ pulp regeneration. Postoperative histological observations were performed. Three patients diagnosed with chronic periapical inflammation in a single canal of the anterior teeth and satisfied with the presence of wisdom teeth were recruited, and the CGF-rich autologous pulp transplantation protocol was selected after obtaining informed consent. Result Animal experiments showed no detachment of all SD rat grafts after surgery, a large amount of neovascularization by HE staining, and positive vascular expression by immunohistochemistry for both human CD31 and murine CD31. The three patients were followed at 3 and 6 months after surgery, and all teeth showed improvement in periapical lesions and positive pulp electrical vitality tests. Conclusion The results of animal experiments indicate that isolated pulp can survive and establish a blood supply with the host, and the addition of CGF facilitates regenerative pulp formation. The clinical results also demonstrated that CGF-rich autologous pulp transplantation protocols are a good regenerative pulp therapy (RET) for the treatment of chronic apical periodontitis in anterior teeth.

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“…While cell type proportions offer a view of changes in cell fractions within bulk samples, and cell type signature matrices provide gene expression profiles at the cell type level, neither can delve into the heterogeneity within individual cell types. This single-cell level granularity is critical for deciphering the complexities of diseases and their response to therapies 22 – 26 . The limitations of traditional bulk decomposition methods restrict our ability to fully explore cellular dynamics and to appreciate the unique contributions of each cell to the overall sample profile.…”

Section: Introductionmentioning

confidence: 99%

scSemiProfiler: Advancing large-scale single-cell studies through semi-profiling with deep generative models and active learning

Wang,

Fonseca,

Ding

2024

Nat Commun

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Single-cell sequencing is a crucial tool for dissecting the cellular intricacies of complex diseases. Its prohibitive cost, however, hampers its application in expansive biomedical studies. Traditional cellular deconvolution approaches can infer cell type proportions from more affordable bulk sequencing data, yet they fall short in providing the detailed resolution required for single-cell-level analyses. To overcome this challenge, we introduce “scSemiProfiler”, an innovative computational framework that marries deep generative models with active learning strategies. This method adeptly infers single-cell profiles across large cohorts by fusing bulk sequencing data with targeted single-cell sequencing from a few rigorously chosen representatives. Extensive validation across heterogeneous datasets verifies the precision of our semi-profiling approach, aligning closely with true single-cell profiling data and empowering refined cellular analyses. Originally developed for extensive disease cohorts, “scSemiProfiler” is adaptable for broad applications. It provides a scalable, cost-effective solution for single-cell profiling, facilitating in-depth cellular investigation in various biological domains.

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Determinants of Dental Pulp Stem Cell Heterogeneity

Cited by 9 publications

References 84 publications

The Role of Sensory Nerves in Dental Pulp Homeostasis: Histological Changes and Cellular Consequences after Sensory Denervation

The Role of Sensory Nerves in Dental Pulp Homeostasis: Histological Changes and Cellular Consequences after Sensory Denervation

Pulp regeneration by transplantation of dental pulp with the synergy of concentrate growth factor: An in vitro and in vivo study

scSemiProfiler: Advancing large-scale single-cell studies through semi-profiling with deep generative models and active learning

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