Determinants of genetic structure of the Sub-Saharan parasitic wasp<i>Cotesia sesamiae</i>

Branca, Antoine; Le, BP Ru; Calatayud, Paul‐André; Obonyo, JO; Musyoka, Boaz; Capdevielle‐Dulac, Claire; Kaiser-Arnauld, Laure; Silvain, Jean‐François; Gauthier, Jérémy; Paillusson, Corentin; Gayral, Philippe; Herniou, Elisabeth A.; Dupas, Stéphane

doi:10.1101/194084

Cited by 4 publications

(15 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All field samples from this lineage ( N = 46 in Branca et al., and N = 35 in Kaiser et al., ) were strictly associated with larvae of the host Sesamia nonagrioides (Noctuidae), feeding mainly on the host plant Typha domingensis (Poales, Typhaceae). The remaining four populations of C. sesamiae identified across Africa show variation in geographic distribution, climate preference, symbiotic bacteria or host range (Branca et al., ). However, the main explanatory factor for the genetic structure of C. sesamiae is association with host species (Branca et al., ), revealing the strong selective pressures imposed by the hosts on the wasp populations.…”

Section: Introductionmentioning

confidence: 95%

“…In other Cotesia species, males are able to locate females searching for hosts, because they are attracted by female sex pheromone and host frass on plant material (Xu et al., ). Microsatellite genotyping of over 600 wasps revealed that C. sesamiae exhibits a genetic structure comprising five major populations (Branca et al., ), which vary in their host range (Branca et al., ) and in parasitic success on different hosts (Gitau, Gundersen‐Rindal, Pedroni, Mbugi, & Dupas, ; Mochiah, Ngi‐Song, Overholt, & Botchey, ). These five C. sesamiae populations include one population recently described as a new species, C. typhae (Kaiser et al., ; Kaiser, Dupas, et al., ).…”

Section: Introductionmentioning

confidence: 99%

“…The remaining four populations of C. sesamiae identified across Africa show variation in geographic distribution, climate preference, symbiotic bacteria or host range (Branca et al., ). However, the main explanatory factor for the genetic structure of C. sesamiae is association with host species (Branca et al., ), revealing the strong selective pressures imposed by the hosts on the wasp populations. All populations are generalists of overlapping host range (sampled mostly within the moth genera Sesamia, Chilo and Busseola ) but with clear preferences, for Busseola in the case of the “Kitale” population and for Chilo in the case of the “Mombasa” population (Branca et al., ; Gitau et al., ).…”

Section: Introductionmentioning

confidence: 99%

“…We developed a capture hybridization method (Hancock‐Hanser, Frey, Leslie, & Dutton, ) specifically targeting 300 kb of the genome of the wasp C. sesamiae containing the bracovirus. The targeted macrolocus as well as two dispersed loci were resequenced for 24 samples from C. typhae and from different C. sesamiae populations of variable host range (Branca et al., ). We evaluated the efficiency and the robustness of the approach before undertaking phylogenomic analyses to assign the samples to the five previously identified populations (Branca et al., ).…”

Section: Introductionmentioning

confidence: 99%

“…The targeted macrolocus as well as two dispersed loci were resequenced for 24 samples from C. typhae and from different C. sesamiae populations of variable host range (Branca et al., ). We evaluated the efficiency and the robustness of the approach before undertaking phylogenomic analyses to assign the samples to the five previously identified populations (Branca et al., ). We then searched for molecular signatures of adaptive evolution by analysing differentiation between population F ST and d xy parameters, by estimating the ratio of substitution rates at nonsynonymous and synonymous sites (dN/dS ratio) and by searching for pseudogenization.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Genetic footprints of adaptive divergence in the bracovirus of Cotesia sesamiae identified by targeted resequencing

et al. 2018

Self Cite

View full text Add to dashboard Cite

The African parasitoid wasp Cotesia sesamiae is a generalist species structured in locally adapted populations showing differences in host range. The recent discovery of Cotesia typhae, a specialist, sister species to C. sesamiae, provides a good framework to study the genetic determinants of parasitoid host range. To investigate the genomic bases of divergence between these populations and species, we used a targeted sequencing approach on 24 samples. We targeted the bracovirus genomic region encoding virulence genes involved in the interaction with the lepidopteran hosts of the wasps. High sequencing coverage was obtained for all samples, allowing the study of genetic variation between wasp populations and species. By combining population genetic estimations, such as nucleotide diversity (π), relative differentiation (F ) and absolute divergence (d ), with branch-site dN/dS measures, we identified six of 98 bracovirus genes showing significant divergence and evidence of positive selection. These genes, belonging to different gene families, are potentially involved in host adaptation and in the specialization process. Fine-scale analyses of genetic variation also revealed mutations and large deletions in certain genes inducing pseudogenization and loss of function. The image emerging from these results is that adaptation mediated by bracovirus genes happens through selection of particularly adaptive alleles and loss of nonadaptive genes. These results highlight the central role of the bracovirus in the molecular interactions between the wasps and their hosts and in the evolutionary processes of specialization.

show abstract

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Genetic footprints of adaptive divergence in the bracovirus of Cotesia sesamiae identified by targeted resequencing

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

Genomic divergence footprints in the bracovirus ofCotesia sesamiaeidentified by targeted re-sequencing approach

Gauthier

Gayral

et al. 2017

Preprint

Self Cite

View full text Add to dashboard Cite

27 28The African parasitoid wasp Cotesia sesamiae is structured in contrasted populations showing 29 differences in host range and the recent discovery of a specialist related species, C. typhae, 30 provide a good framework to study the mechanisms that link the parasitoid and their host 31 range. To investigate the genomic bases of divergence between these populations, we used a 32 targeted sequencing approach on 24 samples. We targeted a specific genomic region encoding 33 the bracovirus, which is deeply involved in the interaction with the host.

show abstract

Characterizing virulence differences in a parasitoid wasp through comparative transcriptomic and proteomic

Gornard,

Venon,

Lasfont

et al. 2024

BMC Genomics

View full text Add to dashboard Cite

Background Two strains of the endoparasitoid Cotesia typhae (Hymenoptera: Braconidae) present a differential parasitism success on the host, Sesamia nonagrioides (Lepidoptera: Noctuidae). One is virulent on both permissive and resistant host populations, and the other only on the permissive host. This interaction provides a very interesting frame for studying virulence factors. Here, we used a combination of comparative transcriptomic and proteomic analyses to unravel the molecular basis underlying virulence differences between the strains. Results First, we report that virulence genes are mostly expressed during the pupal stage 24 h before adult emergence of the parasitoid. Especially, 55 proviral genes are up-regulated at this stage, while their expression is only expected in the host. Parasitoid gene expression in the host increases from 24 to 96 h post-parasitism, revealing the expression of 54 proviral genes at early parasitism stage and the active participation of teratocytes to the parasitism success at the late stage. Secondly, comparison between strains reveals differences in venom composition, with 12 proteins showing differential abundance. Proviral expression in the host displays a strong temporal variability, along with differential patterns between strains. Notably, a subset of proviral genes including protein-tyrosine phosphatases is specifically over-expressed in the resistant host parasitized by the less virulent strain, 24 h after parasitism. This result particularly hints at host modulation of proviral expression. Combining proteomic and transcriptomic data at various stages, we identified 8 candidate genes to support the difference in reproductive success of the two strains, one proviral and 7 venom genes, one of them being also produced within the host by the teratocytes. Conclusions This study sheds light on the temporal expression of virulence factors of Cotesia typhae , both in the host and in the parasitoid. It also identifies potential molecular candidates driving differences in parasitism success between two strains. Together, those findings provide a path for further exploration of virulence mechanisms in parasitoid wasps, and offer insights into host-parasitoid coevolution. Supplementary Information The online version contains supplementary material available at 10.1186/s12864-024-10694-4.

show abstract

Determinants of genetic structure of the Sub-Saharan parasitic waspCotesia sesamiae

Abstract: 6Parasitoid life style represents one of the most diversified life history strategies on earth.

Cited by 4 publications

References 77 publications

Genetic footprints of adaptive divergence in the bracovirus of Cotesia sesamiae identified by targeted resequencing

Genetic footprints of adaptive divergence in the bracovirus of Cotesia sesamiae identified by targeted resequencing

Genomic divergence footprints in the bracovirus ofCotesia sesamiaeidentified by targeted re-sequencing approach

Characterizing virulence differences in a parasitoid wasp through comparative transcriptomic and proteomic

Contact Info

Product

Resources

About