Determination and characterization of cysteine, glutathione and phytochelatins (PC2–6) in Lolium perenne L. exposed to Cd stress under ambient and elevated carbon dioxide using HPLC with fluorescence detection

Ju, Xue Hai; Tang, Sheng; Jia, Yan; Guo, Junkang; Ding, Yongzhen; Song, Zhengguo; Zhao, Yujie

doi:10.1016/j.jchromb.2011.04.016

Cited by 34 publications

(17 citation statements)

References 95 publications

(145 reference statements)

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“…Plants were washed with deionized water and divided into roots, rhizomes, stems and leaves. The different tissues were divided in two portions: one was dried at RT for metal quantification (Almeida et al, 2004) and another was stored at À20°C for plants physiological parameters analysis (Ju et al, 2011).…”

Section: Sample Preparationmentioning

confidence: 99%

Copper phytoremediation by a salt marsh plant (Phragmites australis) enhanced by autochthonous bioaugmentation

Oliveira

Mucha

Reis

et al. 2014

Marine Pollution Bulletin

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Section: Sample Preparationmentioning

confidence: 99%

Copper phytoremediation by a salt marsh plant (Phragmites australis) enhanced by autochthonous bioaugmentation

Oliveira

Mucha

Reis

et al. 2014

Marine Pollution Bulletin

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“…Only a slight amount of PLCys seemed to bind directly to the protein. For quantitative analysis, we tried to label PLCys by mBBr, an SH-specific reagent for fluorometric detection [32] , [33] . However, labeled products were noticeably precipitated even when intact PLCys dissolved in DMSO was treated with mBBr.…”

Section: Discussionmentioning

confidence: 99%

Mechanistic investigation of capability of enzymatically synthesized polycysteine to cross-link proteins

Narai-Kanayama

Hanaishi

Asō

2016

Biochemistry and Biophysics Reports

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BackgroundPreviously, we had reported that α-chymotrypsin–catalyzed polymerization of l-cysteine ethyl ester in a frozen buffer provided poly-l-cysteine (PLCys) in good yield, of which degree of polymerization had been determined to be 6–11. Almost all of SH groups in PLCys were in free forms. Such a multi-thiol peptide may cross-link proteins through thiol/disulfide (SH/SS) exchange reactions, considering the knowledge that other synthetic multi-thiol additives changes properties of protein materials.MethodsThis study explored the capability of PLCys to cross-link proteins using lysozyme as a model protein which has four disulfide bonds but no free SH group. The protein was incubated with PLCys at neutral pH and at below 70 °C to avoid PLCys-independent, β-elimination-mediated cross-linkings. Protein polymerization was analyzed by SDS-PAGE and SEC. PLCys peptides involved in the protein polymer, which were released by reduction with dithiothreitol, were analyzed by RP-HPLC.ConclusionsAddition of urea and thermal treatment at 60 °C caused PLCys-induced lysozyme polymerization. Compared with free cysteine, a higher level of PLCys was required for the polymerization probably due to its low water solubility. RP-HPLC analyses suggested that PLCys played a role in the protein polymerization as a cross-linker.General significanceEnzymatically synthesized PLCys shows promise as a peptidic cross-linker for the production of protein polymers with novel physiochemical properties and functionalities.

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“…The use of an analytical technique is able to detect compounds, specifically mass spectrometry. Independent studies showed a sensitive method for determining PCs by HPLC with fluorescence detection [62,63]. A simple sensitive method for the identification, sequencing and quantitative determination of PCs in plants by electrospray tandem mass spectrometry (ESI MS-MS) was showed for different studies [64,65].…”

Section: Determination Of Phytochelatinsmentioning

confidence: 99%

Role of Phytochelatins in Redox Caused Stress in Plants and Animals

Rodrigo¹,

Anjum²,

Heger³

et al. 2016

Abiotic and Biotic Stress in Plants - Recent Advances and Future Perspectives

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Varied environmental compartments such as soil and water potentially contaminated with different metals/metalloids can impact the health of both plants and animals/ humans. Trace amounts of Cu, Mn, Mo, Ni and Zn are beneficial for higher plants, whereas, Cr, Cu, Co, Mn, Mo, Se, V and Zn are known as the micronutrient metal/metalloids for animals/humans. However, elevated levels of the metals/metalloids can cause severe toxic consequences in both plants and animals/humans. Common in plants and animals/humans, phytochelatins PCs , the principal non-protein, S-rich, thiolate peptides, protect through different mechanisms cellular functions and metal/metalloid homeostasis by performing their chelation and/or detoxification. With the major aim of broadening the current knowledge on the subject, this chapter a overviews PCs role and modulation separately in metal/metalloid-exposed plants and animals/humans b discusses major methods for determination of PCs and bioassays for enzymes involved in PC synthesis c evaluates the connection of PCs with bionanoparticles and finally d highlights so far unexplored aspects in the present context.

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Determination and characterization of cysteine, glutathione and phytochelatins (PC2–6) in Lolium perenne L. exposed to Cd stress under ambient and elevated carbon dioxide using HPLC with fluorescence detection

Cited by 34 publications

References 95 publications

Copper phytoremediation by a salt marsh plant (Phragmites australis) enhanced by autochthonous bioaugmentation

Copper phytoremediation by a salt marsh plant (Phragmites australis) enhanced by autochthonous bioaugmentation

Mechanistic investigation of capability of enzymatically synthesized polycysteine to cross-link proteins

Role of Phytochelatins in Redox Caused Stress in Plants and Animals

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