Determination and comparison of flavonoids and anthocyanins in Chinese sugarcane tips, stems, roots and leaves

Abstract

The flavonoids and anthocyanins in Chinese sugarcane (Saccharum sinensis Roxb.) tips, stems, roots and leaves were separately analyzed by HPLC-UV diode array detector, respectively. The results indicated that the content of flavonoids in sugarcane leaves was considerably high in comparison with previous reports in Brazil sugarcane (S. officinarum L.) leaves. Moreover, the content of flavonoids in sugarcane tips and roots was also high in comparison with sugarcane stems. For another, the content of anthocyanins in sugarcane roots was higher than that in other parts of the sugarcane, such as leaves, tips and stems. In addition, two anthocyanins, named petunidin 3-O-(6''-succinyl)-rhamnoside and cyanidin-3-O-glucoside, were first identified from S. sinensis by HPLC-UV diode array detector and HPLC-MS/MS.

Fast determination of anthocyanins and free pelargonidin in fruits, fruit juices, and fruit wines by high-performance liquid chromatography using a core-shell column

“…As seen in the figure, five anthocyanins and free pelargonidin were fast separated within 9.5 min by a segmented gradient elution of water/formic acid mixture (90:10, v/v, pH 1.3) and acetonitrile using a Poroshell 120 SB-C 18 core-shell column. It represented approximately a 2-to 5.5fold reduction in run-to-run analysis time from existing chromatographic methods for analysis of anthocyanins [8][9][10][11][12][13][14][15][16][17][24][25][26][27][28][29].…”

“…To date, several analytical methods have been reported for identification and quantification of anthocyanins in different sources. HPLC separation coupled to UV or photodiode array detection has a leading position among these studies [8][9][10][11][12][13][14][15][16][17]. Additionally, methods employing UHPLC [18][19][20], nano-LC [21], CZE [22], and CCC [23] have been developed for the determination of anthocyanins.…”

“…In the liquid chromatographic methods reported in the literature flow rates from 0.5 to 1.5 mL/min are used, column temperature ranges from 25 to 50ЊC and run-to-run analysis time is generally from 40 to 110 min, which is timeconsuming for routine analysis [8][9][10][11][12][13][14][15][16][17][27][28][29].…”

Rapid quantitative analysis of individual anthocyanin content based on high‐performance liquid chromatography with diode array detection with the pH differential method

“…HPLC with UV/Vis detection or diode array detection (DAD) is one of most important chromatography‐based techniques, since it permits separation, identification, and quantification of individual anthocyanins, without requiring excessive purity of the anthocyanin samples . The application of HPLC–DAD–MS using ESI has successfully identified anthocyanins in fruit juices and numerous plants . Both 1D and 2D NMR spectroscopy can accurately identify individual anthocyanins in natural plants and obtain more sufficient structural information of purified anthocyanins than others, but not quantify certain anthocyanins content.…”

“…The linearity of calibration curves was observed in the range of 0.02–200 ng/mL. Li plotted a calibration curve using standard solutions of cyaniding 3‐glucoside to analyze anthocyanin content in Chinese sugarcane tips, stems, roots, and leaves by HPLC–DAD. However, as described, pure anthocyanin standards are necessary to perform the quantification, which are generally expensive and lack of availability.…”