Determination and Comparison of In Vitro Radical Scavenging Activity of Both Garlic Oil and Aqueous Garlic Extracts and Their In Vivo Antioxidant Effect on Schistosomiasis Disease in Mice

Sadrefozalayi, Somayye; Aslanıpour, Behnaz; Alan, Murat; Çalan, Mehmet

doi:10.24925/turjaf.v6i7.820-827.1647

Cited by 6 publications

(8 citation statements)

References 36 publications

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“…AL had a more significant effect on RBCs and HCT of the infected mice than PZQ and CU, which may be due to a reduction of worms and egg load. Consistently with this result, it was reported previously that S. mansoni infected groups treated with garlic oil or aqueous garlic extracts (or a mixture of both) showed a significant improvement in HGB and HCT in comparison with infected-untreated animals [ 19 ]. Also, CU-infected treated mice showed an increase in the values of RBCs and HCT after two weeks of treatment [ 24 , 25 ].…”

Section: Discussionsupporting

confidence: 89%

“…Infected groups treated with PZQ, AL, or CU showed improvement in these parameters in comparison with infected-untreated mice. Garlic and its derivatives (as well as CU) were investigated previously as antischistosomal drugs, and they were also found to act as proper antioxidants [ 10 , 19 , 24 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

“…Allicin (AL) (the main active ingredient of Allium sativum ) is considered to be the most critical alkaloid that is generally presumed to be responsible for their useful characteristics [ 17 ]. Most of the studies dealt with garlic itself or its aqueous or oil extracts as a medication of Schistosomiasis mansoni [ 18 , 19 ], and they were efficient in the elimination of worms and enhancement of blood indices and biochemical parameters. Serially, AL significantly caused a reduction in the worm burden, serum concentrations of liver fibrosis markers, and proinflammatory cytokines [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

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Parasitological and Biochemical Efficacy of the Active Ingredients of Allium sativum and Curcuma longa in Schistosoma mansoni Infected Mice

et al. 2021

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The active ingredients allicin and curcumin have a wide range of actions against fungi, bacteria, and helminths. Therefore, the study was aimed to evaluate the efficacy of allicin (AL) and curcumin (CU) as antischistosomal drugs and their biochemical effects in normal and Schistosoma mansoni-infected mice. Praziquantel (PZQ) was administrated for two successive days while AL or CU was given for two weeks from the week 7th postinfection (PI). The possible effect of different regimens on Schistosoma worms was evaluated by measuring the percentage of the recovered worms, tissue egg load, and oogram pattern. Serum alanine transaminase activity and levels of triglycerides, cholesterol, and uric acid were measured. Liver tissue malondialdehyde and reduced glutathione levels besides, the activities of glutathione-S-transferase, superoxide dismutase and catalase were assessed for the oxidative/antioxidant condition. DNA electrophoresis of liver tissue was used to indicate the degree of fragmentation. There was a significant reduction in the recovered worms and egg load, with a marked change of oogram pattern in all treated groups with PZQ, AL, and CU in comparison with infected-untreated mice. PZQ, AL, and CU prevented most of the hematological and biochemical disorders, as well as significantly improved the antioxidant capacity and enhanced DNA fragmentation in the liver tissue of schistosomiasis mice compared to the infected-untreated group. These promising results suggest that AL and CU are efficient as antischistosomal drugs, and it would be beneficial to test their combination to understand the mechanism of action and the proper period of treatment leading to the best result.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Parasitological and Biochemical Efficacy of the Active Ingredients of Allium sativum and Curcuma longa in Schistosoma mansoni Infected Mice

et al. 2021

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“…Some researchers have indicated that the extract of garlic shows high scavenging of free radicals and therefore has powerful antioxidant ability [ 304 , 305 ]. Furthermore, other studies have reported that garlic shows antioxidant ability including one research which analyzed this ability using in vivo experiments [ 71 , 306 ]. Amino acids such as alliin represent one of the important components of garlic [ 307 ].…”

Section: Antimicrobial Agentmentioning

confidence: 99%

Antioxidant, Antimicrobial and Antiviral Properties of Herbal Materials

et al. 2020

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Recently, increasing public concern about hygiene has been driving many studies to investigate antimicrobial and antiviral agents. However, the use of any antimicrobial agents must be limited due to their possible toxic or harmful effects. In recent years, due to previous antibiotics’ lesser side effects, the use of herbal materials instead of synthetic or chemical drugs is increasing. Herbal materials are found in medicines. Herbs can be used in the form of plant extracts or as their active components. Furthermore, most of the world’s populations used herbal materials due to their strong antimicrobial properties and primary healthcare benefits. For example, herbs are an excellent material to replace nanosilver as an antibiotic and antiviral agent. The use of nanosilver involves an ROS-mediated mechanism that might lead to oxidative stress-related cancer, cytotoxicity, and heart diseases. Oxidative stress further leads to increased ROS production and also delays the cellular processes involved in wound healing. Therefore, existing antibiotic drugs can be replaced with biomaterials such as herbal medicine with high antimicrobial, antiviral, and antioxidant activity. This review paper highlights the antibacterial, antiviral, and radical scavenger (antioxidant) properties of herbal materials. Antimicrobial activity, radical scavenger ability, the potential for antimicrobial, antiviral, and anticancer agents, and efficacy in eliminating bacteria and viruses and scavenging free radicals in herbal materials are discussed in this review. The presented herbal antimicrobial agents in this review include clove, portulaca, tribulus, eryngium, cinnamon, turmeric, ginger, thyme, pennyroyal, mint, fennel, chamomile, burdock, eucalyptus, primrose, lemon balm, mallow, and garlic, which are all summarized.

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“…Allicin (AL) is the main active ingredient of Allium sativum (commonly known as garlic) and is considered responsible for many of the beneficial effects associated with this plant ( 12 – 16 ). While many in vitro studies have examined the efficacy of garlic extract as an antischistosomiasis drug ( 17 , 18 ), few have studied the effects of AL in isolation ( 19 ). Curcumin (CU), the active ingredient of Curcuma longa (commonly known as turmeric) is a phenolic compound and a yellow pigment often used as a spice and food colorant ( 20 ).…”

Section: Introductionmentioning

confidence: 99%

The in Vitro Antischistosomal Activity and Genotoxicity of the Active Ingredients of Allium sativum (allicin) and Curcuma longa (curcumin)

Rashed¹,

Almaaty²,

Soliman³

et al. 2021

IJPA

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Background: In this study, we assessed the in vitro antischistosomal activity of the active ingredients of Allium sativum (allicin) and Curcuma longa (curcumin) on Schistosoma mansoni. Methods: This study was conducted in Faculty of Science, Port said University, Egypt (2018). Adult worms were exposed to a range of concentrations of AL or CU, and worm survival was assessed 24 h post-exposure to calculate the lethal concentration of the compounds. Scanning electron microscopy was used to assess ultrastructural changes in the surface of AL- or CU- treated worms. The genotoxicities of AL and CU on S. mansoni were determined by DNA fragmentation analysis. Results: We determined the concentrations of AL and CU required to kill 50% of S. mansoni (LC50). The LC50 of AL was 8.66 µL/mL, whereas 100% mortality of S. mansoni was achieved by AL at concentrations of 50 µL/mL. The LC50 of CU was 87.25 µL/mL, with the highest mortality of 91.3% seen after 24 h exposure to 100 µg/mL CU. Ultrastructural studies revealed that exposure to either AL or CU led to mild or severe surface damage to S. mansion, respectively. The degree of damage in the worms was sex-dependent. Interestingly, while CU exposure resulted in DNA fragmentation in S. mansoni worms, we observed no genotoxic effects of AL. Conclusion: Both AL and CU exhibit antischistosomal activity; the study provided evidence suggesting that these compounds act through distinct mechanisms. These promising results encourage further investigation into these compounds as potential antischistosomal agents, either alone or as complementary treatments to praziquantel.

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Determination and Comparison of In Vitro Radical Scavenging Activity of Both Garlic Oil and Aqueous Garlic Extracts and Their In Vivo Antioxidant Effect on Schistosomiasis Disease in Mice

Cited by 6 publications

References 36 publications

Parasitological and Biochemical Efficacy of the Active Ingredients of Allium sativum and Curcuma longa in Schistosoma mansoni Infected Mice

Parasitological and Biochemical Efficacy of the Active Ingredients of Allium sativum and Curcuma longa in Schistosoma mansoni Infected Mice

Antioxidant, Antimicrobial and Antiviral Properties of Herbal Materials

The in Vitro Antischistosomal Activity and Genotoxicity of the Active Ingredients of Allium sativum (allicin) and Curcuma longa (curcumin)

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