Determination and pharmacokinetic study of unbound cefepime in rat bile by liquid chromatography with on-line microdialysis

Determination of cefepime in plasma and cerebrospinal fluid by micellar electrokinetic chromatography with direct sample injection

“…Microbiological [7,8], spectrophotometric [9] and highperformance liquid chromatography (HPLC) [10][11][12][13][14], and voltammetric and polarographic techniques [15,16] have been proposed for the determination of cefepime in biological samples. In general, bioassays and spectrometry lack specificity, and cannot distinguish multiple antibiotics or active metabolites from the parent compound.…”

“…These analytical methods required a more complicated procedure for sample cleanup. On-line microdialysis coupled with HPLC is also used for measuring unbound cefepime in rat bile [14].…”

Crosstalk the Microdialysis in Scientific Research: from Principle to its Applications

“…The bile duct drug determination not only can understand the drugs secretion conditions, but also can be used to study enterohepatic circulation. The microdialysis technology was applied in the bile duct pharmacokinetic studies and demonstrates that this bile microdialysis method will not affect the bile duct fluid loss and normal secretion, therefore can be long-term, continuously used to collect the determination the bile duct original unbound drugs and the metabolites [113][114][115].…”

“…Chang et al [115,116] used the HPLC analysis coupled with the online microdialysis technique to determine the rats bile duct unbound cefepine pharmacokinetics. The microdialysis probe was implanted into the bile duct, then injected the cefepine intravenously, according to the 50 mg/kg administration, the cefepine in the dialysate solution can be quantification determination.…”

Assaying protein unbound drugs using microdialysis techniques

“…Therefore, catheterization of the duct near the hilum of the liver will allow the collection of pure bile (Fig. 7) [46,[58][59][60][61][62][63][64]. For bile duct cannulation, a midline abdominal incision of about 2 cm is made in an anaesthetized rat.…”