Determination and pharmacokinetic study of dauricine in rat plasma by UPLC–MS/MS

Geng, Peiwu; Zhang, Jing; Chen, Bingbao; Wang, Qianqian; Wang, Shuanghu; Wen, Congcong

doi:10.1556/1326.2017.00118

Cited by 10 publications

(4 citation statements)

References 20 publications

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“…Liquid chromatography conditions separate isocorynoxeine from the endogenous interfering substances as much as possible from the analyte retention time [29][30][31][32][33][34]. In the investigation of chromatographic conditions, mobile phase systems such as methanol-water, acetonitrile-water, methanol-0.1% formic acid, and acetonitrile-0.1% formic acid were investigated.…”

Section: Resultsmentioning

confidence: 99%

Pharmacokinetics of isocorynoxeine in rat plasma after intraperitoneal administration by UPLC–MS/MS

Zhan

Wei

Ren

et al. 2020

Acta Chromatographica

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Isocorynoxeine is one of the main alkaloids in Chinese medicinal herbs, and has pharmacological activities such as antihypertensive, sedative, anticonvulsant, and neuronal protection. It is an effective component of Uncaria for the treatment of hypertension. In this study, we used a fast and sensitive ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) to detect isocorynoxeine in rat plasma and investigated its pharmacokinetics in rats. Six rats were given isocorynoxeine (15 mg/kg) by intraperitoneal (i.p.) administration. Blood (100 μL) was withdrawn from the caudal vein at 5 and 30 min and 1, 2, 4, 6, 8, 12, and 24 h after administration. Chromatographic separation was achieved using a UPLC BEH C18 column using a mobile phase of acetonitrile–0.1% formic acid with gradient elution. Electrospray ionization (ESI) tandem mass spectrometry in the multiple reaction monitoring (MRM) mode with positive ionization was applied. Intra-day and inter-day precisions (relative standard deviation, %RSD) of isocorynoxeine in rat plasma were lower than 12%. The method was successfully applied in the pharmacokinetics of isocorynoxeine in rats after intraperitoneal administration. The t1/2 of isocorynoxeine is 4.9 ± 2.1 h, which indicates quick elimination.

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Section: Resultsmentioning

confidence: 99%

Pharmacokinetics of isocorynoxeine in rat plasma after intraperitoneal administration by UPLC–MS/MS

Zhan

Wei

Ren

et al. 2020

Acta Chromatographica

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“…Optimum liquid chromatography conditions separate the endogenous interfering substances from the analyte and internal standard retention times as far as possible (Ma et al, ; Jin et al, ; Wang et al, 2017; Geng et al, ). We tried acetonitrile–0.1% formic acid, acetonitrile–10 mmol/L ammonium acetate (containing 0.1% formic acid), methanol–0.1% formic acid and methanol–10 mmol/L ammonium acetate (containing 0.1% formic acid), using gradient elution.…”

Section: Resultsmentioning

confidence: 99%

Pharmacokinetics and bioavailability of gelsenicine in mice by UPLC–MS/MS

Jin

et al. 2018

Biomedical Chromatography

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Gelsenicine is an indole alkaloid isolated from Gelsemium elegans Benth. In recent years, the role of G. elegans Benth preparations in anti-tumor, analgesic, dilatation and dermatological treatment has attracted attention, and it has been applied clinically, but it is easy to cause poisoning with its use. An UPLC-MS/MS method was established to determine the gelsenicine in mouse blood, and the pharmacokinetics of gelsenicine after intravenous (0.1 mg/kg) and intragastric (0.5 and 1 mg/kg) administration was studied. Deltalin was used as internal standard; a UPLC BEH C 18 column was used for chromatographic separation. The mobile phase consisted of acetonitrile and 10 mmol/L ammonium acetate (0.1% formic acid) with a gradient elution flow rate of 0.4 mL/min. Multiple reaction monitoring mode was used for quantitative analysis of gelsenicine in electrospray ionization positive interface. Proteins from mouse blood were removed by acetonitrile precipitation. A validation of this method was performed in accordance with the US Food and Drug Administration guidelines. In the concentration range of 0.05-100 ng/mL, the gelsenicine in the mouse blood was linear (r > 0.995), and the lower limit of quantification was 0.05 ng/mL. In the mouse blood, the intra-day precision RSD was <12%, the inter-day precision RSD was <15%, the accuracy ranged from 89.8 to 112.3%, the average recovery was >76.8%, and the matrix effect was between 103.7 and 108.4%, which meet the pharmacokinetic research requirements of gelsenicine. The UPLC-MS/MS method is sensitive, rapid and selective, and has been successfully applied to the pharmacokinetic study of gelsenicine in mice. The absolute bioavailability of gelsenicine is 1.13%.

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“…The choice of positive and negative electrodes for electrospray ESI is often evaluated in methodology [13, 14]. As an alkaloid and alkaline compound, delavinone is more suitable for ESI positive electrode detection.…”

Section: Discussionmentioning

confidence: 99%

Pharmacokinetic Study of Delavinone in Mice after Intravenous and Oral Administration by UPLC-MS/MS

Wang

Zhang

Zheng

et al. 2019

BioMed Research International

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Thirty-one compounds, including delavinone, were isolated from the methanol extract of F. cirrhosa by modern chromatographic techniques. The pharmacological action of Fritillaria is widely used in clinical practice. However, the pharmacokinetic studies on delavinone have not been reported. Therefore, the chemical constituents of this species were investigated. Therefore, it is necessary to establish an analytical method to monitor the concentration of delavinone. An UPLC-MS/MS method was established to determine delavinone in the mouse blood, and the pharmacokinetics of delavinone after intravenous (1.0 mg/kg) and intragastric (2.5, 10.0 mg/kg) administration were studied. The lower limit of quantification was 1.0 ng/mL. The intraday and interday precision RSD were less than 13%, the accuracy ranged from 96.8% to 104.9%, the average recovery was better than 80.6%, and the matrix effect was between 88.8% and 103.4%. The UPLC-MS/MS method has been successfully applied to the pharmacokinetics of delavinone in mice. The noncompartment model was used to fit the main pharmacokinetic parameters. It was found that AUC in mice was higher than that in mice given orally, and the bioavailability of delavinone was 12.4%.

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Determination and pharmacokinetic study of dauricine in rat plasma by UPLC–MS/MS

Cited by 10 publications

References 20 publications

Pharmacokinetics of isocorynoxeine in rat plasma after intraperitoneal administration by UPLC–MS/MS

Pharmacokinetics of isocorynoxeine in rat plasma after intraperitoneal administration by UPLC–MS/MS

Pharmacokinetics and bioavailability of gelsenicine in mice by UPLC–MS/MS

Pharmacokinetic Study of Delavinone in Mice after Intravenous and Oral Administration by UPLC-MS/MS

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