Determination and pharmacokinetics of engeletin in rat plasma by ultra-high performance liquid chromatography with tandem mass spectrometry

Ye, Weijian; Chen, Ruijie; Wei, Sun; Huang, Chengke; Lin, Xiaofeng; Dong, Yaoyao; Wen, Congcong; Wang, Xianqin

doi:10.1016/j.jchromb.2017.06.018

Cited by 45 publications

(25 citation statements)

References 18 publications

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“…There are many plasma pretreatment methods for pharmacokinetic study, e.g., protein precipitation and liquid–liquid extraction [ 20 ]. An effective pretreatment could eliminate endogenous matrix components and reduce the consumption of preparation time.…”

Section: Resultsmentioning

confidence: 99%

A Comparison of Solubility, Stability, and Bioavailability between Astilbin and Neoastilbin Isolated from Smilax glabra Rhizoma

et al. 2020

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Astilbin and neoastilbin are two flavonoid stereoisomers. In the present study, their solubility, stability, and bioavailability were compared in a rat. The results revealed that the water solubility of astilbin and neoastilbin was 132.72 μg/mL and 217.16 μg/mL, respectively. The oil–water distribution coefficient (log P) of astilbin and neoastilbin in simulated gastric fluid (SGF) was 1.57 and 1.39, and in simulated intestinal fluid (SIF) was 1.09 and 0.98, respectively. In SIF, about 78.6% astilbin remained after 4 h of incubation at 37 °C, while this value was 88.3% for neoastilbin. Most of the degraded astilbin and neoastilbin were isomerized into their cis-trans-isomer, namely neoisoastilbin and isoastilbin, respectively, and the decomposed parts were rare. For bioavailability comparison in a rat, an HPLC method for trace amounts of astilbin and neoastilbin determination in plasma was developed, and the pretreatment of plasma was optimized. A pharmacokinetic study showed that the absolute bioavailability of astilbin and neoastilbin in a rat showed no significant difference with values of 0.30% and 0.28%, respectively.

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Section: Resultsmentioning

confidence: 99%

A Comparison of Solubility, Stability, and Bioavailability between Astilbin and Neoastilbin Isolated from Smilax glabra Rhizoma

et al. 2020

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“…In positive electrospray ionization, byakangelicol is more sensitive than in negative ionization. Different chromatographic conditions were used to optimize the sensitivity, chromatographic peak type, and analysis speed of the method [17][18][19][20][21]. In this work, we used acetonitrile-10 mmol/L ammonium acetate (containing 0.1% formic acid), acetonitrile-0.1% formic acid, methanol-0.1% formic acid, and methanol-10 mmol/L ammonium acetate (containing 0.1% formic acid) as mobile phase.…”

Section: Resultsmentioning

confidence: 99%

Pharmacokinetic UPLC–MS/MS studies on byakangelicol after oral and intravenous administration to rats

Bao

Huang

Mao

et al. 2020

Acta Chromatographica

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Byakangelicol is one of coumarins from Baizhi and has been shown to inhibit the release of PGE2 from human lung epithelial A549 cells in a dose-dependent manner. A sensitive ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was developed and full validated for the quantification of byakangelicol in rat plasma. The pharmacokinetics of byakangelicol after both intravenous (5 mg/kg) and oral (15 mg/kg) administrations were studied. Chromatographic separation was performed on an ultra-performance liquid chromatography ethylene bridged hybrid (UPLC BEH) C18 column with acetonitrile and 0.1% formic acid as the mobile phase at a flow rate of 0.4 mL/min; fargesin was used as the internal standard (IS). The following quantitative analysis of byakangelicol was utilized in the multiple reaction monitoring mode. The samples were extracted from rat plasma via protein precipitation using acetonitrile. In the concentration range of 1–2000 ng/mL, the method correlated linearity (r > 0.995) with a lower limit of quantitation (LLOQ) of 1 ng/mL. Intra-day precision was less than 11%, and inter-day precision was less than 12%. The accuracy was between 92.0% and 108.7%, the recovery was better than 89.6%, and the matrix effect was between 85.9% and 98.6%. The method was successfully applied to a pharmacokinetic study of byakangelicol after intravenous and oral administration, and the absolute bioavailability was 3.6%.

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“…A previous report found T max and t 1/2 values for astilbin of 0.43 and 2.5 h, respectively, after administration of astilbin at a single dose of 40 mg/kg (Liang et al, ). Ye et al () reported that engeletin in rat plasma showed rapid absorption ( T max of 0.26 h) and t 1/2 values of 3.686 h after oral administration. Several reports have suggested that resveratrol exhibits a short half‐life of about 8–14 min, with poor bioavailability in animals and humans (Asensi et al, ; Marier et al, ; Walle et al, ).…”

Section: Resultsmentioning

confidence: 99%

“…Liang et al () employed an LC–electrospray ionization–MS method to assay astilbin and 3′‐O‐methylastilbin in rat plasma. The pharmacokinetics of engeletin in rat plasma were determined by a method that combines ultra‐high‐performance liquid chromatography with tandem mass spectrometry (Ye et al, ). Several methods have been applied to measure resveratrol in humans or animal plasma, revealing high absorption but very low bioavailability (Asensi et al, ; Goldberg, Yan, & Soleas, ; Marier et al, ; Walle, Hsieh, DeLegge, Oatis, & Walle, ).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous ultra‐performance liquid chromatography–tandem mass spectrometry determination of six components in rat plasma after oral administration of Smilacis glabrae Roxb. extract

Xie

Shi

et al. 2019

Biomedical Chromatography

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In this study, an accurate and reliable method of ultra-performance liquid chromatography coupled with a triple-quadrupole tandem mass spectrometry was firstly developed and fully validated for the simultaneous determination of epicatechin, neoastilbin, astilbin, isoastilbin, engeletin and resveratrol in rat plasma after administration of Smilacis glabrae Roxb. extract. Naringenin was used as an internal standard (IS). The analyte and IS were separated on a C 18 column by gradient elution with a mobile phase of acetonitrile-0.3% acetic acid at a flow rate of 0.25 mL/min for a total run time of 8 min. The method was validated in terms of selectivity, linearity, precision, accuracy, extraction recovery, matrix effect and stability. The developed method was successfully applied to determine the main pharmacokinetic parameters of six components in rat plasma.

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Determination and pharmacokinetics of engeletin in rat plasma by ultra-high performance liquid chromatography with tandem mass spectrometry

Cited by 45 publications

References 18 publications

A Comparison of Solubility, Stability, and Bioavailability between Astilbin and Neoastilbin Isolated from Smilax glabra Rhizoma

A Comparison of Solubility, Stability, and Bioavailability between Astilbin and Neoastilbin Isolated from Smilax glabra Rhizoma

Pharmacokinetic UPLC–MS/MS studies on byakangelicol after oral and intravenous administration to rats

Simultaneous ultra‐performance liquid chromatography–tandem mass spectrometry determination of six components in rat plasma after oral administration of Smilacis glabrae Roxb. extract

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