Determination and pharmacokinetics of gastrodin and p-hydroxybenzylalcohol after oral administration of Gastrodia elata Bl. extract in rats by high-performance liquid chromatography–electrospray ionization mass spectrometric method

Determination and pharmacokinetics of gastrodin in human plasma by HPLC coupled with photodiode array detector

“…The quantitative data suggested that the LLOQ of 50 ng/ml met the criteria. This method obtained the similar sensitivity with the analysis by microdialysis sampling system and UV detector [13], and it is more sensitive than that with UV detector [12,14,15], but less sensitive than that coupled with MS [16,17]. In all, the method is sensitive enough to determine GAS concentrations after an oral administration of GAS capsule.…”

“…Different mobile phases were previously used to obtain well separation of GAS from endogenous-related substances [12][13][14][15][16][17][18][19]. In present method, several mobile phases were also investigated to optimize analytical performance.…”

“…Good chromatographic results could be obtained for GAS with the extracts of sample deproteinized merely by methanol, ethanol, acetonitrile or perchloric acid [12][13][14][15][16][17][18][19]. In present method, ethanol was used as the precipitant and the ratio of ethanol to plasma was explored.…”

“…The methods of HPLC coupled with UV [12][13][14][15][16], electrospray ionization mass spectrometry (ESI-MS) [17], and ESI-MS-MS [18,19] were established and applied for determination and pharmacokinetic studies of GAS in dog plasma [12] and rat biological samples [13][14][15][16][17][18][19], such as rat plasma, blood, brain, brain dialysate, cerebrospinal fluid (CSF), bile, liver, urine and faeces. The previous results suggest that GAS was rapidly eliminated from the plasma, quickly distributed to brain tissues and bile, and quickly transformed into p-hydroxybenzylalcohol (HBA) after administration; the majority of GAS was excreted to urine but not bile [12][13][14][15][16][17][18][19]. These pharmacokinetic studies were all in animal models, there has been few report on pharmacokinetics of GAS in human body.…”

4-Hydroxybenzyl alcohol derivatives and their sedative–hypnotic activities

“…Gastrodin (GAS) is another chief active ingredient of G. elata, and studies have also reported that GAS is metabolized to HBA in the body and then plays pharmacological roles. 21 Meanwhile, HBA was chosen as one of the standard compounds to evaluate the quality of G. elata. 22 Modern pharmacological experiments have demonstrated that HBA apparently possesses the ability to adjust the central nervous system, including sedation, 23 anticonvulsion, 23 anti-depressant, 24 and anti-neuroinammation activities, 25 facilitating memory and neuroprotection, 26,27 and it is oen used as a precursor compound through structural modication to discover more active compounds.…”