Determination and qualitative confirmation of melengestrol acetate residues in beef fat by electron capture gas chromatography and gas chromatographic/chemical ionization mass spectrometry

Neidert, Eli; Milward, Leo J.; Salisbury, C. D. C.; Gurprasad, Narine P.; Saschenbrecker, Peter W

doi:10.1021/jf00094a013

Cited by 13 publications

(3 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Melengestrol acetate (MGA) was metabolized to 2β,15β-dihydroxy methyl MGA, 6-hydroxy methyl-MGA, 15β-hydroxy-MGA, and 2β-hydroxy MGA in a vitro system prepared from cattle, and the most active metabolite among them was 2β-hydroxy MGA showing 9-times less potency than MGA (WHO, 2004) . The residue level found in Canadian beef heifers treated with MGA at a rate of 0.40 mg/animal per day during 1982~1984 was 2.8 pg/kg as a mean value (ranging < 2 to 28.7 pg/kg) , and 4.6% of all samples had MGA residues of more than 10.0 pg/kg of fat (Neidert et al, 1990) .…”

Section: Potential Human Health Impacts Of Growth Hormones Used In Fomentioning

confidence: 95%

“…Melengestrol is a synthetic progestogen administered orally as a feed additive to improve feed efficiency. The approved feeding doses are in a range of 0.25~0.50 mg/heifer per day during the fattening and finishing periods (Neidert et al, 1990) . Its activity is revealed via a high affinity for progesterone receptors as well as increases in prolactin secretion and the activation of estrogen receptors (Perry et al, 2005) .…”

Section: Potential Human Health Impacts Of Growth Hormones Used In Fomentioning

confidence: 99%

See 1 more Smart Citation

Risk Assessment of Growth Hormones and Antimicrobial Residues in Meat

Jeong¹,

Kang²,

Lim³

et al. 2010

Toxicological Research

109

View full text Add to dashboard Cite

Growth promoters including hormonal substances and antibiotics are used legally and illegally in food producing animals for the growth promotion of livestock animals. Hormonal substances still under debate in terms of their human health impacts are estradiol-17β, progesterone, testosterone, zeranol, trenbolone, and melengestrol acetate (MGA) . Many of the risk assessment results of natural steroid hormones have presented negligible impacts when they are used under good veterinary practices. For synthetic hormonelike substances, ADIs and MRLs have been established for food safety along with the approval of animal treatment. Small amounts of antibiotics added to feedstuff present growth promotion effects via the prevention of infectious diseases at doses lower than therapeutic dose. The induction of antimicrobial resistant bacteria and the disruption of normal human intestinal flora are major concerns in terms of human health impact. Regulatory guidance such as ADIs and MRLs fully reflect the impact on human gastrointestinal microflora. However, before deciding on any risk management options, risk assessments of antimicrobial resistance require large-scale evidence regarding the relationship between antimicrobial use in food-producing animals and the occurrence of antimicrobial resistance in human pathogens. In this article, the risk profiles of hormonal and antibacterial growth promoters are provided based on recent toxicity and human exposure information, and recommendations for risk management to prevent human health impacts by the use of growth promoters are also presented.

show abstract

Section: Potential Human Health Impacts Of Growth Hormones Used In Fomentioning

confidence: 95%

Section: Potential Human Health Impacts Of Growth Hormones Used In Fomentioning

confidence: 99%

Risk Assessment of Growth Hormones and Antimicrobial Residues in Meat

Jeong¹,

Kang²,

Lim³

et al. 2010

Toxicological Research

109

View full text Add to dashboard Cite

show abstract

“…1 In the literature several other methods for the detection of one or of a group of substances, e.g., oestrogens in biological matrices, have been published. [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] There is, however, a need for validated multi-residue methods for the detection of anabolic steroids, in which new compounds, introduced on the black market, can be integrated. Another necessity in the framework of accreditation is validation of the developed methods and of the results obtained.…”

Section: Introductionmentioning

confidence: 99%

Validation of multi-residue methods for the detection of anabolic steroids by GC-MS in muscle tissues and urine samples from cattle†

Daeseleire¹,

Vandeputte²,

Peteghem³

1998

Analyst

View full text Add to dashboard Cite

The use of anabolic steroids is prohibited in the European Community by Directive 96/22/EC and the control of compliance is regulated by Directive 96/23/EC. Multi-residue methods are necessary for screening for the use of forbidden substances. Because accreditation is gaining more and more importance, validation of the methods used and of the results obtained has become indispensable. The developed GC-MS methods for the detection of anabolic steroids in urine and muscle tissue were validated with regard to the following parameters: specificity, recovery at the 2 micrograms kg-1 level and limit of detection. For urine the recoveries ranged from 17 to 81% and for muscle tissue from 26 to 65%. The limit of detection ranged from 0.1 to 2.6 micrograms kg-1 for urine and from 0.3 to 4.6 micrograms kg-1 for muscle tissue. Specificity was guaranteed in both matrices by the selection of four specific ions. Blank samples were evaluated for interferences and it could be concluded that in no case did the four selected ions appear simultaneously at the correct retention time. The practicability of the criteria for low resolution mass spectrometry set in Decision 93/256 in the low micrograms kg-1 range is also briefly discussed.

show abstract

Food analysis and chemical residues in muscle foods

Ellis¹

1994

Quality Attributes and Their Measurement in Meat, Poultry and Fish Products

View full text Add to dashboard Cite

Determination and qualitative confirmation of melengestrol acetate residues in beef fat by electron capture gas chromatography and gas chromatographic/chemical ionization mass spectrometry

Abstract: High-per-formance liquid chromatographic method for the quantification of cholesterol epoxides in spray-dried egg. J. Agrie.

Cited by 13 publications

References 6 publications

Risk Assessment of Growth Hormones and Antimicrobial Residues in Meat

Risk Assessment of Growth Hormones and Antimicrobial Residues in Meat

Validation of multi-residue methods for the detection of anabolic steroids by GC-MS in muscle tissues and urine samples from cattle†

Food analysis and chemical residues in muscle foods

Contact Info

Product

Resources

About