Determination and quantification of dimethyl sulphoxide by HPLC

Thumm, W.; Freitag, D.; Kettrup, A.

doi:10.1007/bf02327979

Cited by 11 publications

(9 citation statements)

References 6 publications

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“…8,9 Since DMA, DMF and DMSO display high absorption at low UV wavelength, analysis could be conducted by liquid chromatography coupled with UV detection. Several high performance liquid chromatography (HPLC) methods have already been developed for the quantification of DMSO in environmental samples, 10,11 or in drug substance. 12 Based on these previous works, a run-time optimization was conceivable using an ultra-high performance liquid chromatography (UHPLC) system.…”

Section: Introductionmentioning

confidence: 99%

Ultra‐fast liquid chromatography method coupled with ultraviolet detection to quantify dimethylsulfoxide, dimethylformamide and dimethylacetamide in short half‐lives radiopharmaceuticals

Fouque

Rusu

Huguet

et al. 2021

Labelled Comp Radiopharmac

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Radiolabelling with short half-lives radionuclides (e.g., fluorine-18 and carbon-11) must be as efficient and as fast as possible. Nucleophilic radiofluorinations and radiomethylations are conducted in polar aprotic solvents, such as dimethylsulfoxyde (DMSO), N,N-dimethylformamide (DMF) and N,Ndimethylacetamide (DMA), at high temperature. Those solvents are classified as toxic according to the ICH guidelines and must be evaluated in drug such as radiopharmaceuticals. Headspace gas chromatography is the standard method for the quantification of residual solvents but is not optimized for a rapid quantification of low vapor pressure solvents such as DMSO, DMF and DMA in radiopharmaceuticals. Direct injection gas chromatography is an interesting option without incubation step but the analysis run-time remains beyond 10 min long. In consequence, we developed a very simple ultra-high performance liquid chromatography method coupled with UV detection. Following the EMA requirements, we successfully validated a 3-min run-time analysis for quantification of three solvents in short half-lives radiopharmaceuticals.We currently use this method for the quality control of radiopharmaceuticals produced in our PET center.

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Section: Introductionmentioning

confidence: 99%

Ultra‐fast liquid chromatography method coupled with ultraviolet detection to quantify dimethylsulfoxide, dimethylformamide and dimethylacetamide in short half‐lives radiopharmaceuticals

Fouque

Rusu

Huguet

et al. 2021

Labelled Comp Radiopharmac

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show abstract

“…The concentration of DMSO in the filtrate and retentate was measured by reverse‐phase high‐performance liquid chromatography (HPLC) using an Agilent 1200 series device (Agilent Technologies UK Ltd, Craven Arms, UK) under the control of the Agilent Chemstation software. A 4 × 125 mm (internal diameter × length) Nucleosil C18 column with a particle size of 5 μm (Sigma‐Aldrich) was used for the stationary phase, while the mobile phase was based on a previously published protocol (Thumm et al ., ). Briefly, an aliquot was taken from the filtrate or retentate sample and diluted 1:20 in 0.1% ( v /v) trifluoroacetic acid (TFA) (Sigma‐Aldrich).…”

Section: Methodsmentioning

confidence: 97%

A novel filtration system for point of care washing of cellular therapy products

Tostoes

Dodgson²,

Weil

et al. 2016

J Tissue Eng Regen Med

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The cell therapy industry would greatly benefit from a simple point of care solution to remove dimethylsulphoxide (DMSO) from small-volume thawed cell suspensions before injection. A novel dead-end filtration device has been designed and validated, which takes advantage of the higher density of thawed cell suspensions to remove the DMSO and protein impurities from the cell suspension without fouling the filter membrane. The filter was designed to avoid fluid circuits and minimize the surface area that is contacted by the cell suspension, thus reducing cell losses by design. The filtration process was established through optimization of the fluid flow configuration, backflush cycles and filter geometry. Overall, this novel filtration device allows for a 1 ml of thawed cryopreserved cell suspensions, containing 10 cells of a fetal lung fibroblast cell line (MRC-5), to be washed in less than 30 min. More than 95% of the DMSO and up to 94% of the albumin-fluorescein-isothiocyanate content can be removed while the viable cell recovery is higher than 80%. It is also demonstrated that this system can be used for bone marrow-derived human mesenchymal stem cells with more than 73% cell recovery and 85% DMSO reduction. This is the first time that a dead end (normal) filtration process has been used to successfully wash high-density human cell suspensions. In practice, this novel solid-liquid separation technology fills the need for small-volume washing in closed processing systems for cellular therapies. Copyright © 2016 John Wiley & Sons, Ltd.

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“…To allow the drug to incubate with the polymer, the mixture was swirled at room temperature for 24 h. The mixture was subsequently dialyzed against DI water overnight in a dialysis bag (Spectra/Por® 12,000–14,000 MWCO, Spectrum Laboratories, USA). The DI water was changed every day until the amount of DMSO was lower than the limit of detection (0.5 mg/L) [26] . A milky solution was obtained and stored at 4 ºC.…”

Section: Methodsmentioning

confidence: 99%

Estrogenic activity and toxicity screening of Damnacanthal nanospheres and their metabolites assessed using an in vitro bioluminescent yeast assay

Chaisutatip

Rojanapanthu²,

Treesuppharat³

et al. 2022

Toxicology Reports

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Determination and quantification of dimethyl sulphoxide by HPLC

Cited by 11 publications

References 6 publications

Ultra‐fast liquid chromatography method coupled with ultraviolet detection to quantify dimethylsulfoxide, dimethylformamide and dimethylacetamide in short half‐lives radiopharmaceuticals

Ultra‐fast liquid chromatography method coupled with ultraviolet detection to quantify dimethylsulfoxide, dimethylformamide and dimethylacetamide in short half‐lives radiopharmaceuticals

A novel filtration system for point of care washing of cellular therapy products

Estrogenic activity and toxicity screening of Damnacanthal nanospheres and their metabolites assessed using an in vitro bioluminescent yeast assay

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