Determination, Isolation, and Identification of Aucubin and Verbascoside in the Leaves of Iraqi Plantago Lancoleta L. Using Different Detecting Methods

Abstract

Objective: Plantago lanceoleta L. (ribwort plantain) is one of the important medicinal herbs which is widespread fortune available in Iraq, that have a wide range of medicinal properties. The aim of this work was to determine, isolate and identify verbascoside and aucubin in Iraqi P. lanceoleta L. by using different chromatographic and spectrometric methods.
 Methods: Verbascoside and aucubin were isolated and quantified by preparative TLC, and then they were determined by the high-performance thin-layer chromatography (HPTLC) fingerprinting. Aucubin and catalpol in the plant extract were analyzed by liquid chromatography-mass spectrometry (LC-MS); aucubin and verbascoside that isolated from the plant sample were examined by fourier-transform infrared spectroscopy (FT-IR) and LC-MS, respectively.
 Results: The result showed that the Iraqi P. lanceoleta L. contains 1.74 percent (verbascoside) and 0.24 percent (aucubin) of dry powdered leaves. Each TLC-isolated compound showed a single spot on the HPTLC plate, which give an idea about the purity of the isolated compound. Aucubin (with catalpol) and verbascoside both are detected by LC-MS in different ionization mode. Many functional groups were identified in the TLC-isolated aucubin by FT-IR.
 Conclusion: The Iraqi P. lanceoleta L. showed a high content of verbasoside, and it is a very rich source for this compound, which can be easily isolated by TLC and subjected to many pharmacological studies. The extract of the young leaves of this plant gave a little amount of aucubin, and it is easy to obtain a higher content from the older leaves.

Study on extraction and antibacterial activity of aucubin from Eucommia ulmoides seed-draff waste biomass

“…The broad and strong absorption peak at 3354 cm −1 was assigned to the stretching vibration of multiple O-H in AU, and the stretching absorption of –CH 2 was at 2916 cm −1 . One can observe a special peak at 1651 cm −1 which could belong to C=C stretching vibration [ 48 ]. The absorption peak at 1486 cm −1 and 1363 cm −1 were corresponding to the bending vibration of –CH 2 .…”

“…The absorption peak at 1486 cm −1 and 1363 cm −1 were corresponding to the bending vibration of –CH 2 . Particularly, the band at 1054 cm −1 could be attributed to –C–O bond, which had redshifted from 1012 cm −1 to 1021 cm −1 gradually as the AU concentration increased [ 48 ]. This indicates the formation of hydrogen bonds between AU, and supports our observation via the 1 H NMR spectroscopy.…”

Selected Plants as Sources of Natural and Active Ingredients for Cosmetics of the Future

“…Moreover, it alleviates symptoms of irritable bowel syndrome, hemorrhoids, and inflammation of the cervix or bladder. It also provides an astringent effect and stimulates wound healing [89].…”

“…Acetoside ester, also known as verbascoside, is used as the main ingredient in syrup against cold [84]. Verbascoside is also applied in other pharmaceutical preparations used to treat neurodegenerative diseases and pain [89]. There have been some reports of the development of liquid crystals containing plantain extract.…”