1993
DOI: 10.1002/bmc.1130070111
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Determination of 1,5‐Anhydroglucitol in urine by high performance liquid chromatography and an enzyme sensor

Abstract: A simple high performance liquid chromatographic method combined with an enzyme sensor has been developed to measure 1,5-anhydroglucitol in urine. The enzyme sensor consists of a hydrogen peroxide electrode and a chitosan membrane of an immobilized pyranose oxidase. As the system does not resist interfering substances, urine samples are first purified by passing them through a two-layer column packed with (1) strongly basic anion (OH- form, the upper layer) and (2) strongly acidic cation (H+ form, the lower la… Show more

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Cited by 9 publications
(4 citation statements)
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“…26-29 Minimally-invasive carbon-fiber microelectrodes (~ 7 μm in diameter) were modified with a non-toxic chitosan matrix using electrodeposition in order to confine GOx in close proximity to the electrode surface. Figure 1 A depicts the electrodeposition process.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…26-29 Minimally-invasive carbon-fiber microelectrodes (~ 7 μm in diameter) were modified with a non-toxic chitosan matrix using electrodeposition in order to confine GOx in close proximity to the electrode surface. Figure 1 A depicts the electrodeposition process.…”
Section: Resultsmentioning
confidence: 99%
“…Chitosan is a natural polysaccharide derived from chitin, which has a high affinity for proteins and has been used extensively for enzyme immobilization. Minimally invasive carbon-fiber microelectrodes (∼7 μm in diameter) were modified with a nontoxic chitosan matrix using electrodeposition in order to confine GOx in close proximity to the electrode surface. Figure A depicts the electrodeposition process.…”
Section: Resultsmentioning
confidence: 99%
“…Since its first characterization by Janssen and Ruelius, POx has received attention as the key biocatalysts in various biotechnological applications; especially analytical applications in clinical chemistry, in monitoring industrial processes, in food preservation and in synthetic carbohydrate chemistry [25][26][27][28][29]. POx has also been used as a biological component in biosensor [18,19,24,27,[30][31][32][33][34][35]. Despite the large-scale industrial use of GOx, there are two main potential advantages in using POx instead of GOx.…”
Section: Introductionmentioning
confidence: 99%
“…The 1,5-AG concentration in serum has been determined by high-performance liquid chromatography (9,13,14) and gasliquid chromatography (10,17,21), but the specificities for 1,5-AG of these methods are relatively low and the procedure, which involves assaying large number of samples, is too tedious. There have been some reports about the enzymes which exhibit activity with 1,5-AG.…”
mentioning
confidence: 99%