Determination of 17<i>α</i>-Methyltestosterone in Freshwater Samples of Tilapia Farming by High Performance Liquid Chromatography

Abstract

17α-methyltestosterone is used to induce the sex reversal of Tilapia sp. to obtain cultures mono-sex to an economically viable. This practice may lead to environmental contamination and problems in human health. Therefore methods need to be developed to detect residues of 17α-methyltestosterone in aqueous matrices. A simple high-performance liquid chromatographic method using ultraviolet detection (245 nm) and testosterone as internal standard has been developed for the monitoring 17α-methyltestosterone in freshwater samples of tilapia aquaculture. The method described involves limited sample preparation as it includes a filtration followed by a single solid-phase extraction step using C18 cartridge. Validation data indicated that the HPLC-UV method for 17α-methyltestosterone determination in the concentration range of 50 -2000 μg/L provided good linearity, sensitivity, accuracy and precision. Method performance was efficiently applied to monitoring the freshwater samples of fish ponds and the surrounding aquatic channels.

Redox-Sensitive and Hyaluronic Acid-Functionalized Nanoparticles for Improving Breast Cancer Treatment by Cytoplasmic 17α-Methyltestosterone Delivery

“…MT is utilized during menopause as a supplement to typical hormone substitution therapy. This substance with a weak dependence on the androgen receptor is an orally active synthetic androgen, which is currently the only androgen in the management of estrogen-androgen Hormone Replacement Therapy (HRT) in the United States with Food and Drug Administration (FDA)-approval [3][4][5]. Studies suggest that dwarfed children and adults with metastatic breast carcinoma subjected to MT showed a reduction in the protein-bound iodine of serum and also in the uptake of radioiodine [6].…”

Kinetic studies using a linear regression analysis for a sorption phenomenon of 17a-methyltestosterone by Salvinia cucullata in an active plant reactor

“…Then, the cartridge was eluted with 4 mL of acetonitrile at a flow rate of 2 mL/min. The volume of the elute solution was adjusted with acetonitrile to 5 mL [2]. The detection method was developed using a Varian Pro Star HPLC system with quaternary pumps, autosampler, column oven, UV detector, and degasser, which were controlled by Varian Star software.…”

“…The chromatographic separation was achieved using a C18 reverse-phase (RP-C 18) column (ACE, 5 μm particle size, 250 × 4.6 mm) at 25 C. In this study, injections of samples were performed either manually or automatically using 20 μL loops on an autosampler. The optimum wavelength for the simultaneous determination of MT was selected and conducted with UV detector at 245 nm [2]. The analytes were separated by running a mobile phase, which consisted of acetonitrile and DI water.…”

“…The analytes were separated by running a mobile phase, which consisted of acetonitrile and DI water. In this study, the ratio of acetonitrile to DI water was modified from that proposed by Barbosa et al [2] to ACN:DI 70:30 (v/v) with a higher flow rate of 2 mL/min.…”

“…To obtain monosex-male cultures using an economically-viable method, mixing fry feed with 60 mg of MT per 1 kg of feed was conducted. Improper discharge of this wastewater may lead to surface water contamination, which bring about environmental concerns due to its toxicity and biodiversity deterioration [2]. Many researchers have studied the effect of MT on reproductive systems of various types of animals [3][4][5].…”