Determination of 2-substituted benzothiazoles of industrial use from water by liquid chromatography/electrospray ionization tandem mass spectrometry

Abstract

A method was developed for the analysis of 2‐substituted benzothiazoles from wastewaters by reversed‐phase liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI‐MS/MS). While benzothiazole (BT), 2‐amino‐ (ABT), 2‐methyl‐, 2‐thiocyanomethylthio‐ and 2‐methylthiobenzothiazole were separated with a methanol gradient acidified with formic acid and detected in the positive mode, 2‐mercapto‐ and 2‐hydroxybenzothiazole (OHBT) as well as benzothiazole‐2‐sulfonic acid (BTSA) were separated with the addition of ammonium acetate and detected in the negative ion mode. Detection limits range from 10 ng L−1 for ABT to 200 ng L−1 for OHBT after direct injection of 100 µL sample volumes, while BT requires 2.5 µg L−1. The method is, thus, suitable for direct analysis in wastewaters. Its application to tannery effluent samples revealed, that, besides a 90–95% elimination of total benzothiazoles, the concentration of OHBT and BTSA increased during the biological treatment. Copyright © 2000 John Wiley & Sons, Ltd.

Benzothiazole Degradation by Rhodococcus pyridinovorans Strain PA: Evidence of a Catechol 1,2-Dioxygenase Activity

“…HPLC-mass spectrometry (MS) analyses were performed with a system consisting of an HP 1100 LC and Quattro LC tandem mass spectrometer (Micromass, Manchester, United Kingdom) as described elsewhere (31) …”

Integrated procedure for determination of endocrine-disrupting activity in surface waters and sediments by use of the biological technique recombinant yeast assay and chemical analysis by LC?ESI-MS

“…non-ionic surfactants, for example nonylphenol ethoxylate (NPEO), and octylphenol ethoxylate (ORFO) and their degradation products nonylphenol (NP) and octylphenol (OP), respectively [45,46,47,48]; 2. bisphenol A (BPA), monomer of polycarbonate, used in packaging, as coating in food cans, and in epoxy resins and many other products [49,50,51]; 3. phthalates, used mainly as plasticizers [27,52,53]; 4. natural and synthetic steroid sex hormones (the natural estrogen estradiol, and its main metabolites estriol and estrone, and the synthetic estrogens ethinyl estradiol (used in pills for contraception and for treatment of menstrual and menopausal disorders) [54] and diethylstilbestrol, the natural hormone progesterone, and the synthetic progestogens norethindrone and levonorgestrel, used in the treatment of infertility, in certain breast and endometrial cancers, etc [55]); and 5. benzothiazoles, industrial chemicals used as vulcanization accelerators in rubber manufacture [56,57,58].…”

Analytical Methods for Polar Pollutants

“…If quaternary ammonium compounds become more polar, and retention in RPLC is difficult to obtain, IP-RPLC can be used (see above). Aromatic nitrogenous compounds like benzothiazoles [69,171] and benzotriazoles [70] can be analyzed with ammonium acetate using ESI in positive mode. The same approach is used for basic pharmaceuticals such as â-2-sympathomimetics and beta-blockers [10,48,158].…”