Determination of 232u and 233u in Irradiated Thorium

Weiler, M.R.; Matsumoto, W.Y.

doi:10.2172/4591212

Cited by 2 publications

(4 citation statements)

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“…Hoping to isolate producing clones in a new milieu [37,45], reducing the opportunities for recruitment of new clones from stem cells, we used both peritoneal memory cells [33] and spleen cells from the same mouse in parallel cell transfers to irradiated hosts (see Fig. 7).…”

Section: Discussionmentioning

confidence: 99%

“…We have now extended the affinity measurements of Trifil [4] to allotypic subpopulations; and observed both properties for periods up to 10 months. In the hope of isolating and following the descendents of a single antibodyproducing clone, we also transferred limiting numbers [32] of either naive or primed spleen cells, or of peritoneal me-mory cells [33], and followed further passages in congeneic hosts. After isoelectric focusing, bands specific for oligo-Dalanine were detected by radioactive labeling, as described by Keck et al [34].…”

Section: Introductionmentioning

confidence: 99%

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Analysis of antibody populations to oligo‐D‐alanine. VIII. Sequential studies on properties of antibody subpopulations: rate constant, affinity, and isoelectric focusing spectrum

et al. 1975

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Allotypically marked antisera to the hapten oligo-D-alanine were studied over periods up to 10 months in individual mice and in serial transfers of both nonimmune and memory cells to irradiated recipients. Parameters observed were divalent association rate constants, affinity and autoradiography of isoelectric focusing (IEF) spectra. The two allotypic subpopulations of heterozygous mice are shown to be independent with respect to affinity. Association rate constant, affinity, and IEF spectra of the antibody populations remain largely stable in most mice over prolonged periods. There is an occasional isolated dramatic change in a single property of one allotypic subpopulation; this may be in the direction of either lower or higher rate constant or affinity. While different recipients of naive spleen cells from a single donor, when immunized, differ one from the other in the measured antibody properties, the characteristics now established in each recipient persist in a lineage of transferred memory cells up to 4 generations, with infrequent changes in isolated properties of only one allotypic subpopulation. The limited changes observed would be in agreement with a model of moderate somatic variability superimposed on a small spectrum of germ line genes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of antibody populations to oligo‐D‐alanine. VIII. Sequential studies on properties of antibody subpopulations: rate constant, affinity, and isoelectric focusing spectrum

et al. 1975

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“…"Allelic repression" appears to be almost universal for genes in Tetrahymena, but parallels may be found in other diploid organisms. Aside from sex-linked repressions, recent pertinent studies are those on gamma-globulin forming cells in the mouse (WEILER 1965) and the rabbit (PERNIS et al, 1965) which express only one of the two alleles at an autosomal locus. H allelic repression in persistent heterozygotes of Salmonella (IINO 1964) may provide another analogy.…”

Section: Discussionmentioning

confidence: 99%

“…From the point of view of ciliate cytogenetics, these observations go far toward a resolution of the persistent and vexatious problem of macronuclear organization (see NANNEY 1964). SONNEBORN'S (1947) genetic studies on Paramecium have long been interpreted as indicating that the macronucleus is composed of diploid subnuclei, but alternative interpretations are difficult to exclude. For example, a macronucleus with large numbers of randomly assorting chromosomes (or haploid subnuclei) would be expected to show considerable phenotypic stability even though constant assortment occurred at each fission (KIMBALL 1943).…”

Section: Discussionmentioning

confidence: 99%

Sequential Nuclear Differentiation in Tetrahymena

Bleyman¹,

Simon²,

Brosi³

1966

Genetics

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UCLEAR differentiation is often responsible for phenotypic differences among genically identical lines in ciliates and is demonstrated by patterns of assortment in vegetative pedigrees. The first example of such a nuclear differentiation ( SONNEBORN 1937( SONNEBORN , 1939 was the "caryonidal inheritance" of mating types in syngen 1 of Paramecium aurelia. In this case, macronuclei usually differentiate in an "all-or-none" fashion early in their development, and mating types are distributed at the cell divisions at which whole new macronuclei are assorted. Macronuclear differentiation is also the basis for mating type determination in the Group B syngens of P. aurelia, which appeared initially to manifest "cytoplasmic inheritance" (SONNEBORN 1954; NANNEY 1957). In syngen 1 of Tetrahymena pyriformis, macronuclear differentiation is again responsible for intraclonal mating type differences, but the diploid subunits of a single compound macronucleus often differentiate to express different mating types and then continue to assort for many cell divisions (NANNEY 1956; ALLEN and NANNEY 1958). Submacronuclear differentiation and vegetative assortment have also been demonstrated to underlie intraclonal variations in the H-immobilization antigens (NANNEY and DUBERT 1960) and the esterase and acid phosphatase isozymes (ALLEN 1960(ALLEN ,1961 ALLEN, MISCH and MORRISON 1963).

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Determination of 232u and 233u in Irradiated Thorium

Cited by 2 publications

References 0 publications

Analysis of antibody populations to oligo‐D‐alanine. VIII. Sequential studies on properties of antibody subpopulations: rate constant, affinity, and isoelectric focusing spectrum

Analysis of antibody populations to oligo‐D‐alanine. VIII. Sequential studies on properties of antibody subpopulations: rate constant, affinity, and isoelectric focusing spectrum

Sequential Nuclear Differentiation in Tetrahymena

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