Determination of 3-hydroxyisovalerylcarnitine and other acylcarnitine levels using liquid chromatography–tandem mass spectrometry in serum and urine of a patient with multiple carboxylase deficiency

Maeda, Yasuhiro; Ito, Tetsuya; Ohmi, Hironori; Yokoi, Kyoko; Nakajima, Yoko; Ueta, Akihito; Kurono, Yukihisa; Togari, Hajime; Sugiyama, Naruji

doi:10.1016/j.jchromb.2007.11.037

Cited by 34 publications

(23 citation statements)

References 19 publications

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“…LTryptophan-d 5 (L-Trp-d 5 ), used as an internal standard, was purchased from Cambridge Isotope Laboratories, Inc.. Trp and Kyn were measured using an ultra-high-performance liquid chromatography (UPLC) -tandem mass spectrometry (MS-MS, Quattro Premier XE mass spectrometer) system (Waters Corporation) as described previously (29). A 10-mL sample solution that was pretreated by a solid phase extraction (SPE) method using an Oasis MCX 30 mg/1 cc SPE cartridge (Waters Corporation) was injected into an Acquity UPLC BEH C18 column (2 Â 100 mm; Waters Corporation) at room temperature.…”

Section: Measurement Of Serum Trp and Kynmentioning

confidence: 99%

Prognostic Significance of Tryptophan Catabolism in Adult T-cell Leukemia/Lymphoma

Awata

Ishida

Maeda

et al. 2015

Clinical Cancer Research

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Purpose: Indoleamine 2,3-dioxygenase 1 (IDO1: IDO), an enzyme catabolizing tryptophan (Trp) into the kynurenine (Kyn) pathway, is increasingly being recognized as an important microenvironmental factor suppressing antitumor immune responses. The purpose of the present study was to determine the prognostic significance of Trp catabolism in adult T-cell leukemia/lymphoma (ATL).Experimental Design: We quantified serum Trp and Kyn in 96 ATL patients, 38 human T-cell lymphotropic virus type-1 asymptomatic carriers (HTLV-1 ACs), and 40 healthy adult volunteer controls. The relationships between various clinical parameters including overall survival were analyzed. IDO expression was evaluated in the affected lymph nodes of ATL patients.Results: Serum Kyn concentrations and Kyn/Trp ratios were significantly higher in HTLV-1 ACs than healthy controls. Both increased significantly with progression from HTLV-1 AC to ATL. However, there were no significant differences in the serum Trp concentrations between ATL patients, HTLV-1 ACs, and controls. IDO was possibly produced by ATL and/or cells of the microenvironment. Multivariate analyses demonstrated that a high serum Kyn/Trp ratio and high Kyn level, but not a high Trp level, were significantly independent detrimental prognostic factors in ATL, as well as in that subset of patients with aggressive variant ATL.Conclusions: Quantification of serum Kyn and Trp is useful for predicting prognosis of an individual ATL patient. Furthermore, ATL, especially in patients with a high serum Kyn/ Trp ratio, is an appropriate disease for testing novel cancer immunotherapies targeting IDO.

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Section: Measurement Of Serum Trp and Kynmentioning

confidence: 99%

Prognostic Significance of Tryptophan Catabolism in Adult T-cell Leukemia/Lymphoma

Awata

Ishida

Maeda

et al. 2015

Clinical Cancer Research

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“…In addition, these methods are not commonly performed together, so samples are analyzed by separate procedures using different instruments. The reported methods for acylcarnitines are not practical for second-tier testing due to long analysis times [9,18,19], poor chromatography [20], or involve alternative instrumentation such as capillary electrophoresis [11]. There are many methods available for amino acid analysis; however, the majority involves derivatization of samples followed by HPLC-UV, FLD, LC/MS or GC/MS [21].…”

Section: Introductionmentioning

confidence: 99%

A quantitative method for acylcarnitines and amino acids using high resolution chromatography and tandem mass spectrometry in newborn screening dried blood spot analysis

Miller

Poston

Karnes

2012

Journal of Chromatography B

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“…The second-tier test described allows rapid separation and quantification of various C5 acylcarnitines which may underlie elevated C5 levels in routine newborn screening in the original blood sample (Janzen et al 2013). Maeda et al (2008Maeda et al ( , 2007 previously described the chromatographic separation of acylcarnitines from serum and urine. However, their method needs a second sample from the patients (urine or serum) and the sample preparation requires the use of solid phase extraction material (SPE).…”

Section: Discussionmentioning

confidence: 99%

Application of a Second-Tier Newborn Screening Assay for C5 Isoforms

Cloppenborg

Janzen

et al. 2013

JIMD Reports

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We report a case of false-positive metabolic screening for isovaleric acidemia in a newborn due to treatment of the mother with pivalic acid containing antibiotics before delivery. By using a recently established second-tier test based on the tandem-MS technique, we could identify pivalic acid in a dried blood sample taken during routine neonatal screening. Before this second-tier test was initiated, diverse analytical procedures were performed in the baby to rule out isovaleric acidemia and carnitine supplementation was started. This caused additional psychological burden to the family. The direct use of the second-tier test would have avoided these negative consequences of a false-positive screening result.

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Determination of 3-hydroxyisovalerylcarnitine and other acylcarnitine levels using liquid chromatography–tandem mass spectrometry in serum and urine of a patient with multiple carboxylase deficiency

Cited by 34 publications

References 19 publications

Prognostic Significance of Tryptophan Catabolism in Adult T-cell Leukemia/Lymphoma

Prognostic Significance of Tryptophan Catabolism in Adult T-cell Leukemia/Lymphoma

A quantitative method for acylcarnitines and amino acids using high resolution chromatography and tandem mass spectrometry in newborn screening dried blood spot analysis

Application of a Second-Tier Newborn Screening Assay for C5 Isoforms

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